牡丹花中不同形态酚类化合物及其抗氧化和α

摘要： 对丹凤和香玉两种牡丹花中的游离酚、酯键合态酚、糖苷键合态酚和不溶性结合态酚进行提取，测定不同形态酚类化合物中总酚、总黄酮含量及其主要化合物组成和含量，并对其抗氧化活性和α-葡萄糖苷酶抑制活性进行研究。结果表明，丹凤和香玉牡丹花的游离酚的总酚和总黄酮含量最高，总酚含量分别为31.45、32.64 mg/g，总黄酮含量分别为41.11、40.67 mg/g，其次是酯键合态酚和糖苷键合态酚，不溶性结合态酚含量较低；两种牡丹花游离酚均含有17 种成分，其主要成分是白藜芦醇、1,2,3,4,6-O-五没食子酰葡萄糖和大波斯菊苷；酯键合态酚、糖苷键合态酚中主要成分是山柰酚-7-O-β-D-葡萄糖苷和大波斯菊苷。不同形态酚类化合物中游离酚的抗氧化活性最强，丹凤、香玉牡丹花游离酚对1,1-二苯基-2-三硝基苯肼自由基、2,2′-联氮-二(3-乙基苯并噻唑-6-磺酸)阳离子自由基清除能力和铁离子还原能力分别为855.03、367.10、230.54 µmol/g和499.06、290.64、196.39 µmol/g；丹凤和香玉牡丹花的游离酚对α-葡萄糖苷酶抑制活性最强，半抑制浓度分别为12.15、13.87 µg/mL。研究结果对丹凤和香玉多酚利用具有一定的参考价值。

关键词: 牡丹花；游离酚；酯键合态酚；糖苷键合态酚；不溶性结合态酚；抗氧化活性；α-葡萄糖苷酶抑制活性

Abstract: Free phenolics (FP), esterified phenolics (EP), glycosidized phenolics (GP) and insoluble bound phenolics (IBP) were successively extracted from two varieties of peony flowers: Danfeng and Xiangyu. The contents of total phenolics, total flavonoids and the major components in different phenolics fractions were determined, and their antioxidant activity and α-glucosidase inhibitory activity were also studied. It was found that for the two varieties, the contents of total phenols (31.45 and 32.64 mg/g, respectively) and total flavonoids (41.11 and 40.67 mg/g, respectively) in FP were both the highest, followed by EP and GP, and the lower values were found in IBP. Each of the two FPs contained 17 components, with the major ones being resveratrol, 1,2,3,4,6-O-pentagalloylglucose and apigetrin. The major components of EP and GP were kaempferol-7-O-β-D-glucoside and apigetrin. The antioxidant activity of FP was the strongest among the four phenolics fractions. The 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radical scavenging capacity and ferric reducing power of FP were 855.03, 367.10, and 230.54 µmol/g for Danfeng, and 499.06, 290.64, and 196.39 µmol/g for Xiangyu, respectively. For both varieties, FP exhibited the strongest inhibitory activity against α-glucosidase with half maximal inhibitory concentrations of 12.15 and 13.87 μg/mL, respectively. The research results are of reference significance for the utilization of polyphenols in Danfeng and Xiangyu.

Key words: Paeoniasuffruticosa Andr. flowers; free phenolics; esterified phenolics; glycosidized phenolics; insoluble bound phenolics; antioxidant activity; α-glucosidase inhibitory activity

中图分类号: 

TS201.2

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