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溶剂对山茶花提取物中酚类化合物和抗氧化活性的影响

来源：花匠小妙招时间：2025-04-16 12:21

溶剂对山茶花提取物中酚类化合物和抗氧化活性的影响

Zhuoya Xiang, Li Liu, Zhou Xu, Qingbo Kong, Shiling Feng, Tao Chen, Lijun Zhou, Hongyu Yang, Yao Xiao and Chunbang Ding*,

引用次数: 0

摘要

关于山茶花生物活性化合物的组成和活性，现有资料十分有限。本研究的目的是鉴定酚类化合物，并研究不同溶剂（乙醇和甲醇）对山茶花中酚类化合物含量和抗氧化活性的影响。使用 UPLC-Q-TOF-MS/MS 进行的分析表明存在 105 种植物化学物质，其中最常见的化合物是黄酮醇、原花青素和鞣花丹宁。有趣的是，在这些花中首次发现了黄酮醇三糖苷。研究表明，溶剂浓度对总酚类化合物（TPC）、总黄酮类化合物（TFC）和总原花青素含量（TPAC）有显著影响。随着溶剂浓度的增加，总酚类化合物、总黄酮类化合物和原花青素总含量都有显著增加，当乙醇浓度为 70% 时，总酚类化合物、总黄酮类化合物和原花青素总含量达到最大值（138.23 毫克 GAE/g DW、421.62 毫克 RE/g DW、60.77 毫克 PB2E/g DW）。然而，花青素总含量在低浓度时达到最大值（0.49 毫克 CGE/g DW）。通过 DPPH- 分析法（DPPH 自由基清除活性）、ABTS-+ 分析法（ABTS 自由基阳离子清除活性）和 FRAP 分析法（铁还原抗氧化能力）测定的抗氧化活性也呈现出类似的趋势。在 100% 溶剂和 70% 甲醇中观察到的抗氧化活性最高。在 14 种单个酚类化合物中，70% 甲醇产生的 8 种化合物（青花素-3-O-葡萄糖苷、原花青素 B2、原花青素 B4、表儿茶素、芦丁、山奈酚-3-O-芸香糖苷、黄芪甲素和槲皮素）的含量最高。此外，研究还发现表儿茶素是含量最高的酚类化合物，约占 20339.37 μg/g DW。基于这些发现，可以得出结论：70% 的甲醇是从多香果菊花中提取多酚的最有效溶剂。这些结果为进一步研究 C. polyodonta 花提供了化学信息和潜在的抗氧化价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

摘要图片

Solvent Effects on the Phenolic Compounds and Antioxidant Activity Associated with Camellia polyodonta Flower Extracts

Camellia polyodonta flowers contain limited information available regarding the composition of their bioactive compounds and activity. The objective of this study was to identify phenolic compounds and investigate the effect of different solvents (ethanol and methanol) on the phenolic content and antioxidant activity in C. polyodonta flowers. The analysis using UPLC-Q-TOF-MS/MS revealed the presence of 105 phytochemicals and the most common compounds were flavonols, procyanidins, and ellagitannins. Interestingly, flavonol triglycosides were identified for the first time in these flowers. The study demonstrated that the concentration of the solvent had a significant impact on the total phenolic compound (TPC), total flavonoid compound (TFC), and total proanthocyanidin content (TPAC). The TPC, TFC, and TPAC showed a remarkable increase with the increasing concentration of the solvent, reaching their maximum levels (138.23 mg GAE/g DW, 421.62 mg RE/g DW, 60.77 mg PB2E/g DW) at 70% ethanol. However, the total anthocyanin content reached its maximum at low concentrations (0.49 mg CGE/g DW). Similar trends were observed in the antioxidant activity, as measured by the DPPH· assay (DPPH radical scavenging activity), ABTS·+ assay (ABTS radical cation scavenging activity), and FRAP assay (Ferric reducing antioxidant power). The maximum antioxidant activity was observed at 100% solvents and 70% methanol. Among the 14 individual phenolic compounds, 70% methanol yielded the highest content for 8 (cyanidin-3-O-glucoside, procyanidin B2, procyanidin B4, epicatechin, rutin, kaempferol-3-O-rutinoside, astragaline and quercitrin) out of the 14 compounds. Additionally, it was found that epicatechin was the most abundant phenolic compound, accounting for approximately 20339.37 μg/g DW. Based on these findings, it can be concluded that 70% methanol is the most effective solvent for extracting polyphenols from C. polyodonta flowers. These results provided chemical information and potential antioxidant value for further research in C. polyodonta flowers.