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响应面法优化甘草制款冬花微波炮制工艺

来源：花匠小妙招时间：2025-10-24 21:15

目的优化甘草制款冬花的微波炮制工艺。方法以单因素实验作为基础,以紫外分光光度法(UV)和高效液相法(HPLC)测定甘草制款冬花中总生物碱、款冬酮、芦丁和异槲皮苷含量,结合醇浸出物含量的总评归一值为评价指标,对闷润时间、甘草用量、微波火力和炮制时间4个因素进行响应面实验研究,优化甘草制款冬花的微波炮制工艺。结果甘草制款冬花的响应面法得出最佳工艺条件为闷润时间4.5 h、甘草用量15%,微波火力100%,炮制时间83 s,总生物碱、款冬酮、芦丁、异槲皮苷和醇浸出物含量分别为0.002 1%、0.283 6%、0.7%、1.44%和27.6%。结论优选得到的甘草制款冬花炮制工艺合理、稳定、可行,可为甘草制款冬花的工业化生产提供理论依据。

Abstract

OBJECTIVE To optimize the microwave processing technology of Farfarae Flos processed with Glycyrrhizae Radix et Rhizoma. METHODS On the basis of single factor test, the index was evaluated by overall normalized value of content of total alkaloid, coltsfoot ketone, rutin, isoquercitrin and alcohol extract, which were determined by UV and HPLC method, four factors (moist time, the dosage of Glycyrrhizae Radix et Rhizoma, fire and processing time) were studied by response surface methodology. The microwave processing technology of Farfarae Flos processed with Glycyrrhizae Radix et Rhizoma was optimized. RESULTS The optimal parameters of microwave processing technology were as follows: the moist time was 4.5 h, the dosage of Glycyrrhizae Radix et Rhizoma was 15%, the microwave power was 100%, the processing time was 83 s. The contents of total alkaloid, coltsfoot ketone, rutin, isoquercitrin and alcohol extract were 0.002 1%, 0.283 6%, 0.7%, 1.44% and 27.6%, respectively. CONCLUSION The optimized processing technology of Farfarae Flos processed with Glycyrrhizae Radix et Rhizoma is rational, stable and feasible, which can provide a theoretical basis for the industrial production.

关键词

款冬花 /响应面法 /微波炮制 /甘草制 /总评归一值{{custom_keyword}} /

Key words

Farfarae Flos /response surface method /microwave processing /being processed with Glycyrrhizae Radix et Rhizoma /overall normalized value{{custom_keyword}} /

参考文献

[1] XIAO P G. New Compilation of Traditional Chinese Medicine Annals(新编中药志) [M]. Beijing: Chemical Industry Press, 2003.
[2] LI J, ZHANG S, QIN X M, et al. Comparison on chemical constituents in raw and honey baked Farfarae Flos by NMR-based metabolomic approach[J]. Chin Tradit Herb Drugs (中草药), 2015, 46(20):3009-3016.
[3] Ch.P (2015). Vol Ⅰ(中国药典2015年版. 一部)[S]. 2015: 332-333.
[4] QI Y S, LI J, GUO D, et al. Chemical comparison of the stems and leaves of Tussilago farfara L. using NMR-based metabolomics[J]. Chin Pharm J(中国药学杂志), 2019, 54(8):608-613.
[5] GAO H, HUANG Y N, GAO B, et al. α-Glucosidase inhibitory effect by the flower buds of Tussilago farfara L.[J]. Food Chem, 2008, 106(3):1195-1201.
[6] PARK H R, YOO M Y, SEO J H, et al. Sesquiterpenoids isolated from the flower buds of Tussilago farfara L. inhibit diacylglycerol acyltransferase[J]. J Agric Food Chem, 2008, 56(22):10493-10497.
[7] CHENG X Y, ZHANG X, LIAO M, et al. Analysis on chemical composition of Farfarae Flos by UPLC-Q-TOF-MS[J]. Chin Tradit Herb Drugs(中草药), 2017, 48(12):2390-2400.
[8] ZHANG Y. Advances in studies on toxicity, active sites and pharmacological action of Farfarae Flos[J]. China Pract Med(中国实用医药), 2015, 10(35):287-288.
[9] HUI L Q, GAO S R, LIU T, et al. Hepatotoxicity on water extracts and the total alkaloid of Farfarae Flos[J]. Chin J Exp Tradit Med Form (中国实验方剂学杂志), 2012, 18 (4):238-241.
[10] LI J, GAO J N, QIN X M, et al. Toxicity comparison between combination of Asteris Radix with Farfarae Flos and leaves of Tussilago farfara L.[J]. Chin Tradit Herb Drugs(中草药), 2016, 47(24):4379-4387.
[11] CHEN L M, HE Y N, WANG F, et al. Study advances of microwave processing technology in traditional Chinese medicine[J]. China J Chin Mater Med(中国中药杂志), 2020, 45(9):2073-2081.
[12] SONG Y J, GUO T, SUN Z Q, et al. Optimization of microwave processing technology for vinegar Corydalis Rhizoma by response surface methodology[J]. Chin Tradit Herb Drugs(中草药), 2017, 48(20):4261-4267.
[13] TENG Y, YANG H L. Optimal extraction of indirubin from Isatidis Folium based on Plackett-Burman design combined with central composite design-response surface methodology[J]. Chin Tradit Herb Drugs(中草药), 2019, 50(8):1942-1946.
[14] JIANG A, LI G Y, WANG X, et al. Optimization of compounded nanosuspension of paclitaxel and betulinic acid by Box-Behnken design-response surface method[J]. Chin Tradit Herb Drugs(中草药), 2019, 50(4):852-859.
[15] LI M X, ZHOU Z, TIAN S Y, et al. Optimization of toxicity attenuation processing tehnology for Farfarae Flos being processed with Glycyrrhizae Radix et Rhizoma by orthogonal test[J]. Chin J Exp Tradit Med Form(中国实验方剂学杂志), 2016, 22(18):17-20.
[16] WANG M F, LI K, MENG X L, et al. Comparison of total alkaloid content in Tussilago farfara before and after processing[J]. Chin Pharm Aff(中国药事), 2015, 29 (2):178-182.
[17] WU D, LEI C, TANG L. Simultaneous determination of seven constituents in Tussilago farfara by HPLC[J]. Chin Tradit Pat Med(中成药), 2019, 41(5):1080-1084.
[18] JIA Y, XING J, LEI Z H, et al. Current status in studies on quality evaluation methods of Farfarae Flos[J]. Chin Tradit Herb Drugs(中草药), 2017, 48(21):4578-4583.
[19] NIU Z R, LI X D, GU X Z, et al. Preparation of licorice juice as a processing excipient[J]. China J Chin Mater Med(中国中药杂志), 2009, 34(16):2051-2053.
[20] LEI X. Leigong Treatise on the Preparation(General Solution)[雷公炮炙论(通解)][M]. Xi′an: San Qin Press, 2001: 263.
[21] TAN Z C, CHEN H Y, GAO N, et al. Study on microwave processing technology of charred Sophorae Fructus based on response surface methodology[J]. Guangdong Chem Ind(广东化工), 2019, 46(8):90-92.
[22] HUANG X, LIU J, FU X M, et al. Optimization of microwave processing technology for carbonized Gradeniae Fructus by Box-Behnken response surface methodology based on CRITIC weighted evaluation[J]. Chin Tradit Herb Drugs(中草药), 2017, 48(6):1133-1138.
[23] HE Y N, CHEN L M, HUANG W, et al. Study on the influence factors of Aconiti Lateralis Radix Praeparata by microwave processing technology[J]. Chin Tradit Herb Drugs(中草药),2020, 51(12):3157-3164.
[24] SONG Y J, GUO T, WANG M B, et al. Optimization of microwave processing technology for honey Farfarae Flos by response surface method[J]. Chin J New Drugs(中国新药杂志), 2020, 29(6):692-700.
[25] HUANG X, LIU J, FU X M, et al. Correlations between index components and powder color changes in microwave processing of Gardeniae Fructus[J]. Chin J Exp Tradit Med Form(中国实验方剂学杂志), 2017, 23(10):1-6.
[26] HOU A J, GUO X Y, MAN W J, et al. Research progress of chemical compositions and pharmacological effects of Farfarae Flos[J]. Infor Tradit Chin Med (中医药信息), 2019, 36 (1):107-112.
[27] DUAN Y H, ZHANG Y, WANG X, et al. Analysis on liver and renal toxicity of leaves of Tussilago farfara using zebrafish[J]. Chin Tradit Herb Drugs(中草药), 2019, 50(3):669-674.
[28] LI D, ZHANG J, LIANG P, et al. Quality evaluation of Tussilago farfara from different producing areas and different harvesting periods[J]. J Chin Med Mater (中药材), 2015, 38(4):720-722.
[29] YANG M, LIU X B, HUANG Q D. The increasing effect and reducing ill effect principe of compatibility of Radix Aconiti Lateralis Praeparata and Radix Glycyrrhizae[J]. Lishizhen Med Mater Med Res (时珍国医国药), 2003, 14(4): 197-198.
[30] WANG J, DAI L, LI P Y, et al. Study on active ingredients for increased efficacy of compatibility of Penoy and Licorice[J]. Chin J Exp Tradit Med Form (中国实验方剂学杂志), 2014, 20(11): 83-86.