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山楂果胶及其低聚物对高脂饮食小鼠肠道菌群和代谢产物的影响。

来源：花匠小妙招时间：2025-05-20 12:09

山楂果胶及其低聚物对高脂饮食小鼠肠道菌群和代谢产物的影响。

Xiushan Zhang, Yanmin Cui, Zuoyi Zhang, Xin Huang, Xiaowei Zhang, Xiaopei Hu, Tuoping Li and Suhong Li

引用次数: 0

摘要

果胶是一种天然、绿色、廉价的酸性杂多糖。与多糖相比，低聚糖更容易被人体利用，山楂果胶低聚糖（POS）的生理功能可能因其聚合度（DP）而异。因此，我们主要研究不同DP的山楂果胶（HP）和POS对高脂饮食（HFD）引起的肠道菌群紊乱的影响。HP和POS均可改善由HFD引起的体重增加、血脂异常和葡萄糖稳态，并增加血清GLP-1水平。同时，治疗组回肠Gcg和Pcsk1基因表达的增加进一步证实了这一结果。此外，HP和POS减少了某些机会致病菌，同时恢复了肠道微生物群的丰富度和多样性。同时，HP和POS可通过增加claudin-1、occludin、ZO-1和MUC2基因改善肠道屏障功能障碍。此外，粪便代谢组学研究表明，POS可能通过上调9,10- dhome （(12Z)-9,10-二羟基十八烯-12-烯酸）来促进亚油酸合成，改善脂质代谢，而HP则不能。综上所述，研究结果表明，HP和POS均能改善HFD引起的体重表型改变、肠道菌群破坏和代谢物变化。其中，POS的改善效果优于HP，不同DP的POS改善效果存在差异，其中DP 5的POS改善效果最为显著。这一发现加深了对不同POS生物活性的认识，为进一步优化POS作为功能食品的应用提供了重要依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Effects of hawthorn pectin and its oligomers on gut microbiota and metabolites in high-fat diet mice†

Pectin is an acidic heteropolysaccharide with natural, green, and inexpensive characteristics. Compared to polysaccharides, oligosaccharides are more easily utilized by the body, and the physiological function of hawthorn pectin oligosaccharides (POS) may vary depending on their degree of polymerization (DP). Therefore, we mainly studied the effects of hawthorn pectin (HP) and POS with different DP on gut microbiota disorders induced by high-fat diet (HFD). HP and POS both improved weight gain, dyslipidemia, and glucose homeostasis caused by HFD, and increased serum GLP-1 levels. Meanwhile, the increased expression of Gcg and Pcsk1 genes in the ileum of the treatment group further confirmed this result. In addition, HP and POS reduced certain opportunistic pathogens, while restoring the richness and diversity of the gut microbiota. Meanwhile, HP and POS can improve intestinal barrier dysfunction by increasing the claudin-1, occludin, ZO-1, and MUC2 genes. Furthermore, fecal metabolomics suggests that POS may enhance linoleic acid synthesis and improve lipid metabolism by upregulating 9,10-DHOME ((12Z)-9,10-dihydroxyoctadec-12-enoic acid), while HP cannot. Overall, the research results indicate that both HP and POS can improve the weight phenotype changes, gut microbiota disruption, and metabolites changes caused by HFD. Particularly, POS has a better effect than HP, and there are differences in the improvement effect of POS with different DP, among which POS with DP 5 has the most significant improvement effect. This discovery enhances a deeper comprehension of the biological activity of different POS, providing an important basis for further optimizing the application of POS as a functional food.