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光质对火龙果糖分积累及其代谢酶活性的影响

来源：花匠小妙招时间：2025-09-22 12:04

摘要： 以红肉火龙果紫蜜龙为试材,采用蓝光与红光比例为1∶4和1∶2两种光质的发光二极管(light emitting diode,LED)进行补光处理,以不补光为对照,测定果实发育过程中蔗糖、葡萄糖和果糖含量以及蔗糖代谢相关酶——酸性转化酶(AI)、中性转化酶(NI)、蔗糖合成酶(SS)和蔗糖磷酸合成酶(SPS)的活性,并对果实中糖积累与酶活性的关系进行分析。结果表明,蓝红光比例1∶2处理的成熟果实中可溶性固形物、蔗糖含量、SPS和SS活性显著高于其他处理,且AI和NI活性在幼果期后始终显著低于其他处理;除1∶4处理成熟果实中果糖含量和AI活性低于CK处理,其他无显著差异。1∶2的LED补光处理可以在提高春季首批火龙果产量的同时保持品质稳定,实现优质稳产。光质对糖积累的调控机制可能是,红光较少的光质抑制火龙果果实中AI活性,减少蔗糖分解为葡萄糖和果糖,同时调控SPS和SS合成方向的活性,从而促进果实中蔗糖积累,提高可溶性固形物的含量。

关键词: 火龙果, 发光二极管, 果实品质, 糖, 代谢酶

Abstract: In this study, a red pitaya cultivar, Zimilong was used as the experimeatal matertal. Two levels of blue to red light ratios (1∶4 and 1∶2) in light emitting diode (LED) were set up as the treatments, and no artificial light group was the control. By measuring the content of sucrose, glucose, fructose as well as activities of sucrose metabolism-related enzymes in the flesh of pitaya at three important stages, we attempted to preliminarily explore the mechanism of light supplementation regulating sugar accumulation. Results showed that the total soluble solid (TSS) and sucrose content in fruits under LED light supplementation with blue and red light ratio of 1∶2 were higher than other treatments, the activity of sucrose phosphate synthase (SPS) and sucrose synthase (SS) showed the same as well, while the activity of acid invertase (AI) was opposite. There was no significant difference except for fructose content and AI activity in mature fruit in the treatment of 1∶4 was lower than the control. Overall, the sucrose content of the first batch of spring pitaya was improved in the LED light supplementation with blue and red light ratio of 1∶2. Maybe, less red light ratio could promote sucrose, reduced glucose and fructose by inhibiting the activity of AI and promoting the activity of SPS and SS so as to promote sucrose and TSS in fruit.

Key words: pitaya, light emitting diode, fruit quality, sugar, metabolic enzyme

中图分类号:

S667.9

网址: 光质对火龙果糖分积累及其代谢酶活性的影响 https://www.huajiangbk.com/newsview2361100.html

所属分类：花卉