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马齿苋的化学成分及其生物活性研究

来源：花匠小妙招时间：2025-05-01 09:27

摘要：

对中药马齿苋的化学成分特别是有机酸类成分进行深入研究, 并对其抗炎和醌还原酶诱导活性进行筛选。通过聚酰胺、硅胶、葡聚糖凝胶等柱色谱方法进行分离纯化, 利用核磁共振波谱等数据鉴定结构。共分离鉴定了20个化合物, 分别为丁二酸 (1)、丁二酸单甲酯 (2)、L-苹果酸 (3)、L-苹果酸-1-甲酯 (4)、L-苹果酸-4-甲酯 (5)、L-苹果酸二甲酯 (6)、L-柠檬酸-6-乙酯 (7)、L-柠檬酸-1-甲酯 (8)、L-柠檬酸-1,5-二甲酯 (9)、4-羟基-5-甲基-呋喃-3-甲酸 (10)、5-羟甲基糠酸 (11)、硬脂酸 (12)、L-焦谷氨酸 (13)、环-(酪氨酸-亮氨酸) (14)、L-异亮氨酸 (15)、(−)-dehydrovomifoliol (16)、(−)-epiloliolide (17)、3,4-二羟基苯乙醇 (18)、丁二酰亚胺 (19)、尿嘧啶 (20)。其中14个化合物2, 4–8, 10, 11, 13–18为首次从马齿苋中分离得到。化合物18 (12.5 μM)具有抗炎活性, 对脂多糖诱导RAW264.7巨噬细胞中一氧化氮的产生具有强的抑制作用, 且可提高Hepa lclc7 细胞的醌还原酶活性; 化合物16 (50 μM)具有弱的醌还原酶诱导活性, 其它化合物未显示出抗炎和醌还原酶诱导活性。

关键词: 马齿苋, 化学成分, 抗炎, 醌还原酶诱导活性

Abstract:

In the present study, we aimed to intensively study the chemical constituents, especially organic acids from a medicinal plant Portulaca oleracea L., and screen their anti-inflammatory and quinone reductase (QR, a phase II detoxyfication enzyme) inductive activity. A total of 20 compounds were isolated and identified based on spectroscopic methods, as succinic acid (1), mono-methyl succinate (2), L-malic acid (3), L-1-methyl malate (4), L-4-methyl malate (5), L-dimethyl malate (6), L-6-ethyl citrate (7), L-1-methyl citrate (8), L-1,5-dimethyl citrate (9), 4-hydroxy-5-methylfuran-3-carboxylic acid (10), 5-hydroxymethyl-furoic acid (11), stearic acid (12), L-pyroglutamic acid (13), cyclo-(tyrosine-leucine) (14), L-isoleucine (15), (–)-dehydrovomifoliol (16), (–)-epiloliolide (17), 3,4-dihydroxyphenylethanol (18), succinimide (19), and uracil (20). Among them, 14 compounds (2, 4–8, 10, 11, 13–18) were isolated from P. oleracea for the first time. Compound 18 (12.5 μM) exhibited potent anti-inflammatory effect in lipopolysaccharide (LPS)-induced macrophage cells (RAW264.7) by reducing NO production, and it also increased QR activity in Hepa lclc7 cells. Compound 16 (50 μM) showed weak QR inductive activity. None of other compounds showed anti-inflammatory or QR inductive activities.

Key words: Portulaca oleracea, Chemical constituents, Anti-inflammatory, QR inductive activity

中图分类号:

R284

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