超声波辅助提取黑果腺肋花楸花色苷工艺优化及稳定性研究

摘要： 以黑果腺肋花楸为原料，在单因素实验的基础上应用响应面法对其提取条件进行优化，并研究稳定性。优化黑果腺肋花楸花色苷提取条件为乙醇浓度为63%、超声时间为32 min、超声温度为42 ℃、料液比为1∶20（g/mL），在此条件下的黑果腺肋花楸花色苷的提取量为5.61 mg/g。在50 ℃以下的条件下黑果腺肋花楸花色苷稳定性较好，保存率可达98%以上；氧化剂对黑果腺肋花楸花色苷有显著破坏作用；在避光的条件下黑果腺肋花楸花色苷稳定性最好；Ca2+、K+、Mg2+离子对黑果腺肋花楸花色苷几乎无破坏作用，但Fe2+、Cu2+离子的破坏作用较为显著；在偏酸性条件下黑果腺肋花楸花色苷稳定性较好。

关键词: 黑果腺肋花楸, 超声波, 花色苷, 响应面法, 稳定性

Abstract: The extraction conditions for improved yield of anthocyanins from Black Chokeberry (Aronia melanocarpa) were optimized by single factor experiment and response surface method.The stability of anthocyanins was investigated at the same time.The optimal extraction conditions of anthocyanins from Black Chokeberry were as follows:ethanol concentration was 63%,extraction time was 32 min,extraction temperature was 42 ℃,solid-liquid ratio was 1∶20 (g/mL).And the extraction yield of anthocyanins under these conditions was 5.61 mg/g.As the temperature was lower than 50 ℃,the preservation rate of anthocyanins in the crude extract was above 98%.However,the oxidant had a significant effect on the stability of anthocyanins while the stability of anthocyanins was best in dark environment.Ca2+,K+ and Mg2+ had little destructive effect on the anthocyanins while Fe2+ and Cu2+ had significant destructive effect on the anthocyanins.In addition,anthocyanins were more stable under acidic conditions.This study provided important information for the extraction of anthocyanins from Black Chokeberry.

Key words: Black Chokeberry (Aronia melanocarpa), ultrasonic, response surface, anthocyanins, stability

中图分类号: 

TS255.36

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