Biology and Molecular Characterization of Cucurbit leaf crumple virus, an Emergent Cucurbit

摘要

Cucurbit leaf crumple virus (CuLCrV) is an emergent and potentially economically important bipartite begomovirus first identified in volunteer watermelon plants in the Imperial Valley of southern California in 1998. Field surveys indicated that CuLCrV has become established in the Imperial Valley; and field plot studies revealed that CuLCrV primarily infects cucurbits, including cantaloupe, squash, and watermelon. Full-length DNA-A and DNA-B clones of an Imperial Valley isolate of CuLCrV were obtained by polymerase chain reaction (PCR) with overlapping primers. These clones were infectious in various cucurbits and common bean (cv. Topcrop); symptoms included stunted growth and leaf crumple, curl, and chlorosis. CuLCrV was not sap-transmissible, and immunolocalization and DNA in situ hybridization studies revealed that it is phloem-limited. A CuLCrV agroinoculation system was generated, and host range studies revealed differential susceptibility in cucurbits, with squash, watermelon, cantaloupe, and honey-dew melon being most to least susceptible, respectively. Germplasm screening studies identified a number of resistant cantaloupe and honeydew melon cultivars. The genome organization of this CuLCrV isolate (CuLCrV-CA) is similar to other bipartite begomovirases, and phylogenetic analysis placed CuLCrV in the Squash leaf curl virus (SLCV) cluster of New World bipartite begomoviruses. A CuLCrV-specific PCR test was developed which allows for differentiation from other begomoviruses, including SLCV.展开▼

机译：葫芦丝叶片皱缩病毒（CuLCrV）是一种新兴的且具有潜在经济意义的两性begomovirus，最早于1998年在加利福尼亚南部帝国谷的志愿者西瓜植物中鉴定出来。野外调查表明，CuLCrV已在帝国谷中建立。田间研究表明，CuLCrV主要感染葫芦科植物，包括哈密瓜，南瓜和西瓜。通过重叠引物的聚合酶链反应（PCR）获得CuLCrV的Imperial Valley分离株的全长DNA-A和DNA-B克隆。这些克隆在各种葫芦科植物和普通豆（cv。Topcrop）中具有感染力。症状包括生长发育迟缓，叶片皱缩，卷曲和萎黄。 CuLCrV不是汁液可传播的，免疫定位和DNA原位杂交研究表明它是韧皮部有限的。产生了CuLCrV农杆菌接种系统，寄主范围研究显示葫芦科中的敏感性不同，南瓜，西瓜，哈密瓜和蜜露瓜分别最易受影响。种质筛选研究确定了许多抗性哈密瓜和蜜瓜品种。该CuLCrV分离株（CuLCrV-CA）的基因组组织与其他两部分begomovirases类似，系统发育分析将CuLCrV置于新世界两部分begomovirus病毒的南瓜卷叶病毒（SLCV）簇中。开发了CuLCrV特异性PCR试验，该试验可与包括SLCV在内的其他begomovirus病毒区分开。展开▼

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