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夏黑葡萄花青素抗氧化活性及对D

来源：花匠小妙招时间：2025-06-26 16:29

WANG Bing-ya, LIAN Xiu-yi, YANG Chen-bo, GUO Xu-ran, SHEN Pei-hong, LIU Wen-tao, LIU Le. Antioxidant Activity of Summer Black Grape Anthocyanin and Its Protective Effect on D-galactose Induced Aging Mice[J]. Science and Technology of Food Industry, 2020, 41(3): 22-26,32. DOI: 10.13386/j.issn1002-0306.2020.03.005

Citation: WANG Bing-ya, LIAN Xiu-yi, YANG Chen-bo, GUO Xu-ran, SHEN Pei-hong, LIU Wen-tao, LIU Le. Antioxidant Activity of Summer Black Grape Anthocyanin and Its Protective Effect on D-galactose Induced Aging Mice[J]. Science and Technology of Food Industry, 2020, 41(3): 22-26,32. DOI: 10.13386/j.issn1002-0306.2020.03.005

王兵亚1, 连秀仪2, 杨琛擘3, 郭旭冉4, 申培红5, , , 刘文涛4, 刘乐1

1. 郑州大学公共卫生学院, 河南郑州 450001;

2. 河南富景生态旅游开发有限公司, 河南郑州 450001;

3. 郑州大学基础医学院, 河南郑州 450001;

4. 郑州大学材料科学与工程学院, 河南郑州 450001;

5. 郑州大学附属肿瘤医院, 河南郑州 450008

详细信息

作者简介:

王兵亚(1990-),男,硕士,研究方向:营养与食品卫生学,E-mail:wangbingyaleehom@163.com。

通讯作者:

申培红(1967-),女,博士,教授,研究方向:病理学,E-mail:13838122660@163.com。

中图分类号: TS255.1

计量文章访问数: 0303 HTML全文浏览量: 048 PDF下载量: 010 出版历程收稿日期: 2019-05-12 网络出版日期: 2020-11-12 刊出日期: 2020-01-31

Antioxidant Activity of Summer Black Grape Anthocyanin and Its Protective Effect on D-galactose Induced Aging Mice

1. School of Public Health, Zhengzhou University, Zhengzhou 450001, China;

2. Henan Fujing Ecotourism Development Co., Ltd., Zhengzhou 450001, China;

3. School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, China;

4. College of Materials Science and Engineering, Zhengzhou University, Zhengzhou 450001, China;

5. Affiliated Tumor Hospital of Zhengzhou University, Zhengzhou 450008, China

摘要

摘要: 目的:研究夏黑葡萄花青素提取物的抗氧化活性及其抗衰老作用。方法:以夏黑葡萄花青素为研究材料,通过测定其清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基、超氧阴离子、羟基自由基能力和总还原能力来评价其抗氧化活性。用不同剂量夏黑葡萄花青素对通过颈背部注射D-半乳糖构建的40只衰老模型小鼠连续灌胃30 d,禁食不禁水24 h后取血检测各组血清中超氧化物歧化酶(SOD)和丙二醛(MDA)水平;并取大脑和肾脏做苏木精-伊红染色(hematoxylin-eosin staining,HE)病理学切片,观察其组织病理学变化。结果:夏黑葡萄花青素对于DPPH自由基、超氧阴离子、羟基自由基具有较强的清除力。与模型对照组相比,中、高剂量组小鼠血清中的SOD水平显著提高(P<0.05);中、高剂量组小鼠血清中的SOD水平显著高于低剂量组(P<0.05),同时显著降低血清MDA水平值(P<0.05)。从大脑、肾脏组织病理切片看,随着夏黑葡萄花青素处理剂量的增加,神经元细胞形态逐渐规则,数量丰富,细胞间隙变小,细胞数目较多,排列整齐;肾小球数量增多丰富,形态变饱满。结论:夏黑葡萄花素具有一定的抗氧化能力,对D-半乳糖衰老模型小鼠具有较好的保护作用。

Abstract: Object:To study the antioxidant activity and anti-aging effect of summer black grape anthocyanin extract. Methods:The antioxidant activity of summer black grape anthocyanin extract was studied by measuring the ability to scavenge 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)free radicals,superoxide anion,hydroxyl radicals,and total reducing power.40 aging model mice,which were established by injecting D-galactose into the nape of the neck,were fed with different doses of summer black grape anthocyanin for 30 days,and blood samples were taken after 24 h of fasting to detect the levels of superoxide dismutase(SOD)andmalondialdehyde(MDA)in serum. Brain and kidney were taken for hematoxylin-eosinstaining(HE)pathological tissue sections to observe the histopathological changes. Results:Summer black grape anthocyanin had strong scavenging ability against DPPH radicals,superoxide anion,and hydroxyl radicals. Compared with the model group,SOD level in serum of mice was lower than that of medium and high dose groups(P<0.05),while medium and high dose group was higher than that of low dose groups(P<0.05),and significantly reduce the level of serum MDA(P<0.05).From the histopathological sections of brain and kidney,with the increase of anthocyanin dosage,the morphology of neurons gradually became regular and abundant,the cell gap became smaller,the number of cells were larger,arranged neatly,the number of glomeruli was increased and abundant,and the morphology became plump. Conclusion:Summer black grape anthocyanin extract has certain antioxidant ability,and it has a better protective effect on D-galactose aging mice.

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计量文章访问数: HTML全文浏览量: PDF下载量: 出版历程收稿日期: 2019-05-12 网络出版日期: 2020-11-12 刊出日期: 2020-01-31