Screening of Brassica oleracea MLPK

GAO Qiguo;LEI Zhenze;LIANG Yunfei;JIANG Yupeng;ZHU Liquan;College of Horticulture and Landscape Architecture,Southwest University,Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education;College of Agronomy and Biotechnology,Southwest University;  

MLPK(M-locus protein kinase)is a positive key mediator of Brassica self-incompatibility signaling,however,the molecular mechanism of MLPK in mediating Brassica self-incompatibility response is still unknown. Meanwhile,the downstream factors of Brassica self-incompatibility signaling still need to be isolated. Aim to explore the idea and methods to isolate MLPK-interacting proteins,here,the MLPK truncated protein MLPK-T lack of the plasma membrane localization motif was constructed,the interaction of MLPK with ARC1 was proved via conventional yeast two-hybrid system. Then,we obtained 96,101,70 and 62 PUB proteins from B. olercea,B. rapa,Arabidopsis lyrata and A. thaliana through genome-wide identification,amount to 127 PUB proteins containing ARM repeat domains. Based on phylogenetic analysis,we selected 8 PUB proteins,and RT-PCR showed that all these 8 genes expressed in B. oleracea stigmas. Finally,the interaction of MLPK with Bol008579,Bol016165 and Bol023511 were proved through yeast two-hybrid system.

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