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荷叶碳点的制备及在食品胭脂红检测中的应用

来源：花匠小妙招时间：2025-05-10 13:56

DOI:

作者单位:

(1. 贵州中医药大学药学院，贵州贵阳 550025; 2. 贵州中医药大学药食两用资源研究与开发中心，贵州贵阳 550025)

基金项目:

贵州省科技计划项目（编号：黔科合基础-ZK［2022］一般项目468，黔科合基础-ZK［2024］一般项目383）；贵州中医药大学大学生创新创业计划训练项目（编号：贵中医大创合字〔2022〕63号）

Preparation of carbon dots from Folium nelumbinis and their application in the detection of food carmine

Author:

HE Li 1

HE Li

College of Pharmacy , Guizhou University of Traditional Chinese Medicine , Guiyang , Guizhou 550025 , China
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LI Jiaxin 1,2

LI Jiaxin

College of Pharmacy , Guizhou University of Traditional Chinese Medicine , Guiyang , Guizhou 550025 , China;Center for Research and Development of Medicinal and Food Dual Use Resources , Guizhou University of Traditional Chinese Medicine , Guiyang , Guizhou 550025 , China
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HU Chengna 1

HU Chengna

College of Pharmacy , Guizhou University of Traditional Chinese Medicine , Guiyang , Guizhou 550025 , China
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FENG Xiangxin 1

FENG Xiangxin

College of Pharmacy , Guizhou University of Traditional Chinese Medicine , Guiyang , Guizhou 550025 , China
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FENG Tinging 1,2

FENG Tinging

Affiliation:

(1. College of Pharmacy , Guizhou University of Traditional Chinese Medicine , Guiyang , Guizhou 550025 , China; 2. Center for Research and Development of Medicinal and Food Dual Use Resources , Guizhou University of Traditional Chinese Medicine , Guiyang , Guizhou 550025 , China)

摘要 | | 访问统计 | 参考文献 [26] | | || 文章评论

摘要:

［目的］建立一种简便、快速、高效、灵敏的胭脂红检测方法。［方法］以荷叶为碳源，采用溶剂热法制备碳点（FN-CDs），通过单因素试验优化制备条件；研究影响 FN-CDs荧光强度的因素、共存物对检测体系的影响，优化测定条件；将FN-CDs用于食品中胭脂红含量测定并探究其作用机理。［结果］以0.6 g荷叶、0.25 g柠檬酸、45 mL甲酰胺在240 ℃反应 4 h时，FN-CDs荧光值最强。在0~70 μg/mL的胭脂红质量浓度范围内，荧光强度与胭脂红浓度成正比，具有良好线性关系，R2为0.996 4，检出限为 0.57 μg/mL。西柚复果汁饮料和杨梅罐头加标回收率分别为 88.09%~102.49%，97.73%~112.47%。［结论］该检测方法具备灵敏度高、选择性好、准确度高等特点，可应用于食品中胭脂红的快速检测。

Abstract:

［Objective］ To establish a fast,sensitive,and accurate method for detecting carmine.［Methods］ Using Folium Nelumbinis;as the carbon source,carbon dots (FN-CDs ) were prepared using a solvent thermal method,and the preparation conditions were optimized using a single factor test.Study the factors that affect the fluorescence intensity of FN -CDs,the effects of coexisting substances on the detection system,and optimize the measurement conditions;Using FN -CDs for the determination of carmine content in food and exploring its mechanism of action.［Results］］ Using 0.6 g of Folium Nelumbinis,0.25 g of citric acid and 45 mL of formamide were reacted at 240 ℃ for 4 hours,and the fluorescence value of FN -CDs was the strongest.Rouge had a good linear relationship within the concentration range of 0~70 μg/mL,with an R2 of 0.996 4 and a detection limit of 0.57 μg/mL.The spiked recovery rates of grapefruit juice beverage and canned arbutu were 88.09%~102.49% and 97.73%~112.47%,respectively.［Conclusion］ This detection method has the characteristics of high sensitivity,good selectivity and high accuracy,and can be applied to the rapid detection of carmine in food.

引用本文

何莉,李嘉欣,胡成娜,等.荷叶碳点的制备及在食品胭脂红检测中的应用[J].食品与机械,2024,40(11):54-60.
HE Li, LI Jiaxin, HU Chengna, et al. Preparation of carbon dots from Folium nelumbinis and their application in the detection of food carmine[J]. Food & Machinery,2024,40(11):54-60.

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