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埃及栽培的马钱子叶和花的化学成分、抗氧化性和抗老年痴呆活性：体外和硅学

来源：花匠小妙招时间：2025-04-21 23:54

埃及栽培的马钱子叶和花的化学成分、抗氧化性和抗老年痴呆活性：体外和硅学研究

IF 3.4 Q2 PHARMACOLOGY & PHARMACY

引用次数: 0

摘要

背景如今，阿尔茨海默病及其治疗方法已成为全球关注的问题。阿尔茨海默病患者预后较差，需要支持性治疗。本研究评估了马钱子叶和花乙醇提取物（LEE 和 FEE）的抗氧化活性、抗乙酰胆碱酯酶（anti-AChE）、抗丁酰胆碱酯酶（anti-BChE）和 Aβ-淀粉样蛋白-42抑制活性。使用 2,2-二苯基-1-苦基肼水合物、氧自由基吸收能力和铁嗪铁金属螯合试验评估了 LEE 和 FEE 的抗氧化活性。此外，还测定了它们的总黄酮和总酚。采用 LC-MS/Q-TOF 正、负离子模式分析了 LEE 的植物化学成分。结果由于黄酮类化合物含量高，LEE 在所有技术中都表现出较高的抗氧化和抗老年痴呆活性。在抑制 Aβ-淀粉样蛋白-42 方面，LEE 比多奈哌齐和利巴斯的明在抑制丁酰胆碱酯酶方面表现出更好的活性。利用超高效液相色谱-电喷雾离子化-四极杆飞行时间质谱法（UHPLC-ESI-TOF-MS）初步鉴定出 62 种化合物，即 35 种黄酮类化合物、11 种酚酸类化合物、2 种萜类化合物、2 种苯丙类衍生物、7 种多酚类化合物、3 种香豆素类化合物和 2 种有机酸类化合物。部分成分的分子对接研究表明，异鼠李素-O-芸香糖苷、西索特林、3,5,7-三羟基-4'-甲氧基黄酮（diosmetin）、迷迭香酸、山柰酚六糖苷、山柰酚-7-新橙皮苷、醋酸苷、紫杉叶素和芹菜素-O-六糖苷具有抗老年痴呆药物的活性。结论马钱子鳞叶中的次生代谢物有望成为天然抗氧化剂、乙酰胆碱酯酶、丁酰胆碱酯酶和 Aβ-淀粉样蛋白-42抑制剂，有助于治疗阿尔茨海默氏症。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Chemical profile, antioxidant and anti-Alzheimer activity of leaves and flowers of Markhamia lutea cultivated in Egypt: in vitro and in silico studies

Background

Nowadays Alzheimer’s disease and its treatment methods are global concerns. Patients with this disease have poor prognosis and need supportive treatment. The antioxidant activity, anti-acetylcholinesterase (anti-AChE), anti-butyryl cholinesterase (anti-BChE) and Aβ-amyloid-42 inhibition activities of the ethanolic extracts of both leaves and flowers (LEE and FEE) of Markhamia lutea were assessed. The antioxidant activity of LEE and FEE was evaluated using 2,2-diphenyl-1-picryl-hydrazyl-hydrate, oxygen radical absorbance capacity and ferrozine iron metal chelation assays. Additionally, their total flavonoids and total phenolics were determined. The phytochemicals of LEE were analysed using LC–MS/Q-TOF in both positive and negative modes. Also, molecular docking was done for phytochemicals identified in LEE.

Result

LEE exhibited higher antioxidant and anti-Alzheimer activities in all techniques due to its high flavonoids content. LEE showed better activity than donepezil in case of anti-butyryl cholinesterase than both donepezil and rivastigmine in case of Aβ-amyloid-42 inhibition. A total of 62 compounds were tentatively identified using Ultra-performance Liquid Chromatography-Electrospray Ionization-Quadrupole Time-of-Flight Mass Spectrometry (UHPLC–ESI–TOF–MS), viz. 35 flavonoids, 11 phenolic acids, 2 terpenoids, 2 phenylpropanoids derivatives, 7 polyphenols, 3 coumarins and 2 organic acids. The molecular docking of some constituents showed that isorhamnetin-O-rutinoside, sissotrin, 3,5,7-trihydroxy-4'-methoxyflavone (diosmetin), rosmarinic acid, kaempferol hexoside, kampferol-7-neohesperoside, acacetin, taxifolin and apigenin-O-hexoside exert a promising activity as anti-Alzheimer drugs.

Conclusion

The LEE of Markhamia lutea contains secondary metabolites that is promising to act as natural antioxidants, acetylcholinesterase, butyryl cholinesterase and Aβ-amyloid-42 inhibition inhibitors, which can aid in the treatment of Alzheimer’s.

Graphical Abstract

来源期刊

Future Journal of Pharmaceutical Sciences

自引率

0.00%

发文量

审稿时长

23 weeks

期刊介绍： Future Journal of Pharmaceutical Sciences (FJPS) is the official journal of the Future University in Egypt. It is a peer-reviewed, open access journal which publishes original research articles, review articles and case studies on all aspects of pharmaceutical sciences and technologies, pharmacy practice and related clinical aspects, and pharmacy education. The journal publishes articles covering developments in drug absorption and metabolism, pharmacokinetics and dynamics, drug delivery systems, drug targeting and nano-technology. It also covers development of new systems, methods and techniques in pharmacy education and practice. The scope of the journal also extends to cover advancements in toxicology, cell and molecular biology, biomedical research, clinical and pharmaceutical microbiology, pharmaceutical biotechnology, medicinal chemistry, phytochemistry and nutraceuticals.