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萱草花黄酮对免疫性肝损伤小鼠保护作用及机制

来源：花匠小妙招时间：2025-01-18 06:39

摘要: 目的探讨萱草花黄酮对免疫性肝损伤小鼠保护作用及凋亡相关基因bax、bcl-2表达变化。方法使用卡介苗和脂多糖制备小鼠免疫性肝损伤模型，将60只昆明小鼠随机分为对照组、模型组、低、中、高剂量萱草花黄酮（15、30、60 mg/kg）组。苏木素-伊红染色观察肝组织病理损伤程度；比色法测定肝组织中超氧化物歧化酶（SOD）、丙二醛和谷胱甘肽过氧化物酶（GSH-Px）含量；生化分析仪检测血清中谷丙转氨酶（ALT）含量；免疫组化法检测肝脏bax、bcl-2表达。结果与对照组比较，模型组小鼠血清中ALT水平[（105.48±46.34）U/L]、丙二醛含量[（8.57±0.54）nmol/mgprot]明显升高，SOD与GSH-Px活力[分别为（168.14±13.21）、（95.32±1.48）U/mgprot]明显下降（P<0.01）；与模型组比较，30、60 mg/kg萱草花黄酮组小鼠血清中ALT水平[分别为（56.38±26.31）、（59.77±13.34）U/L]、丙二醛含量[分别为（5.37±0.34）、（5.52±0.38）nmol/mgprot]降低（P<0.05），SOD与GSH-Px活力[分别为（197.40±25.22）、（249.41±24.08）与（107.32±1.83）、（114.24±1.41）U/mgprot]升高（P<0.05）；与对照组比较，模型组小鼠肝脏bax基因表达上调，bcl-2基因表达下调（P<0.05）；与模型组比较，萱草花黄酮小鼠肝脏bax表达下调，bcl-2表达上调（P<0.05）。结论萱草花黄酮对小鼠免疫性肝损伤具有一定保护作用，其机制可能与提高小鼠抗氧化能力、下调促凋亡基因bax表达、上调抗凋亡基因bcl-2表达有关。

Abstract: Objective To study protective effect of Hemerocallis citrina baroni flavonids (HCBF) on autoimmune liver injury and the influence of HCBF on expressions of BCL-2-associated X protein (bax) and B cell lymyhoma/leukemia-2 protein (bcl-2) in mice.Methods The diabetic nephropathy (DN) mice model was established by injections of Bacillus Calmette-Guerin (BCG) and lipopolysaccharide (LPS).Sixty male Kunming mice were randomly divided into five groups:a control group,a DN model gourp,and low,moderate,and high treatment groups with HCBF of 15,30,60 mg/kg,respectively.Liver pathological changes were observed with hematoxylin-eosin staining.The contents of superoxide dismutase (SOD),malondialdehyde (MDA) and glutathione-peroxidase (GSH-Px) were measured with colorimetry.Serum alanine aminotransferase (ALT) was detected with biochemistry analyzer.The expressions of bax and bcl-2 were determined with immunohistochemistry.Results Compared to those of the controls,serum ALT (105.48±46.34 U/L) and MDA (8.57±0.54 nmol/mgprot) increased but SOD (168.14±13.21 U/mgprot) and GST-Px (95.32±1.48 U/mgprot) decreased in the mice of model group significantly (P<0.01 for all).Compared to those of the mice of the model group,serum ALT (56.38±26.31 and 59.77±13.34 U/L) and MDA (5.37±0.34 and 5.52±0.38 nmol/mgprot) decreased but SOD (197.40±25.22 and 249.41±24.08 nmol/mgprot) and GST-Px (107.32±1.83 and 114.24±1.41 U/mgprot) increased significantly for the mice of 30 and 60 mg/kg HCBF treatment groups (P<0.05 for all).Compared to the mice in the control,the expression of bax was up-regulated but the expression of bcl-2 was down-regulated for the mice in the model group (both P<0.05).Compared to the mice in the model group,the expression of bax was down-regulated but the expression of bcl-2 was up-regulated for the mice in the HCBF treatment groups (P<0.05 for all).Conclusion HCBF exhibits protective effect on autoimmune liver injury in mice and the effect may relate to the enhancement of antioxidant capacity,downregulation of bax and upregulation of bcl-2 in the mice.

[1] 刘欢,陈英利,张冰,等.酒精性肝损伤的氧化应激和同型半胱氨酸促进肝细胞凋亡及其机制[J].哈尔滨医科大学学报,2015,49(3):219-224. [2] 李素婷,吴淑彦,杜超,等.山楂叶总黄酮对酒精性肝损伤小鼠肝细胞凋亡的影响[J].中国老年学杂志,2015,35(7):1889-1891. [3] 杨社华,葛斌,王艳红,等.马栗籽提取物对急性肝损伤小鼠自由基及TNF-含量的影响[J].中国医院药学杂志,2014,34(17):1453-1456. [4] 王云锋,赵丽晶,徐博,等.萱草花的营养成分和生物活性研究进展[J].吉林医药学院学报,2012,33(1):46-49. [5] 潘红,郝丽静,黄建梅,等.萱草花化学成分研究[J].时珍国医国药,2012,23(9):2186-2187. [6] 安英,沈楠,赵丽晶,等.萱草花总黄酮对四氯化碳诱导大鼠慢性肝纤维化的保护作用[J].时珍国医国药,2015,26(7):1623-1625. [7] 郭冷秋,张颖,张博,等.萱草根及萱草花的化学成分和药理作用研究进展[J].中华中医药学刊,2013,31(1):74-76. [8] 龚晓男,王晶.黄芪甲苷对免疫性肝损伤大鼠保护作用[J].中国公共卫生,2014,30(5):634-636. [9] 杨逸,覃筱燕,郭哲,等.人参皂苷Rg1对小鼠免疫性肝损伤保护作用[J].中国公共卫生,2015,31(3):309-311. [10]

Long Y,Dong X,Yuan Y,et al.Metabolomics changes in a rat model of obstructive jaundice:mapping to metabolism of amino acids,carbohydrates and lipids as well as oxidative stress[J].J Clin Biochem Nutr,2015,57(1):50-59.

[11]

Amorim BO,Covolan L,Ferreira E,et al.Deep brain stimulation induces antiapoptotic and anti-inflammatory effects in epileptic rats[J].J Neuroinflammation,2015,12(1):162.

[12] 秦佩,雷志明,吴双,等.槲皮素对Con A诱导自身免疫性肝损伤作用[J].中国公共卫生,2015,31(6):757-759. [13]

Sen S,Chakraborty R,Thangavel G,et al.Hepatoprotective and antioxidant activity of Karisalai Karpam,a polyherbal Siddha formulation against acetaminophen-induced hepatic damage in rats[J].Anc Sci Life,2015,34(4):198-202.

[14]

Choi EK,Jung H,Kwak KH,et al.Effects of allopurinol and apocynin on renal ischemia-reperfusion injury in rats[J].Transplant Proc,2015,47(6):1633-1638.

[15]

Jiang H,Wang Z,Ma Y,et al.Effects of dietary lycopene supplementation on plasma lipid profile,lipid peroxidation and antioxidant defense system in feedlot Bamei lamb[J].Asian-Australas J Anim Sci,2015,28(7):958-965.

[16]

Kuroda K,Kabata T,Hayashi K,et al.The paracrine effect of adipose-derived stem cells inhibits osteoarthritis progression[J].BMC Musculoskelet Disord,2015,16(1):236.

[17]

Carnet O,Lecomte J,Masset A,et al.Mesenchymal stem cells shed amphiregulin at the surface of lung carcinoma cells in a juxtacrine manner[J].Neoplasia,2015,17(7):552-563

[18] 杨方万,穆茂媛,肖娟娟,等.内质网应激诱导细胞凋亡机制的研究进展[J].医学研究杂志,2014,43(10):176-180.