首页分享南方、试验站和花生根结线虫随机扩增多态性dna标记的研究.docx

南方、试验站和花生根结线虫随机扩增多态性dna标记的研究.docx

来源：花匠小妙招时间：2025-01-05 15:01

南方、试验站和花生根结线虫随机扩增多态性dna标记的研究 rootknotnemat组（meloidol.）是一个缓慢的马蹄莲类组，属于马蹄莲类。开普敦运营和运营的权限与ses%以上的权限之差有关。而且，80%的原始移动生态行为中存在壳和碎片超。而且，80%的移动生态行为中存在壳和碎片中的竞争行为。米。 Effective management of root-knot nematodes often requires rapid and accurate species identification of target nematode populations.Meloidogynespecies are traditionally differentia-ted from each other by morphological characters and isozyme phenotypes. Morphological identification demands considerable skill and could be unreliable due to significant intraspecific morpholo-gical variations inMeloidogynespp. Isozyme phenotyping has been shown to be a valuable tool for precise identification of majorMeloidogynespecies. A limitation of this technique is that juveniles, males and eggs can not be reliably diagnosed, which hinders its use in routine examination of soil samples that often contain only se-cond-stage juveniles. Recent progress in development of PCR-based molecular diagnostics has offered a potential replacement for the traditional procedures. Powers and Harrisdiscriminated single juveniles of five major species ofMeloidogyneby amplification and restriction of the intergenic region between theCOIIandlrRNAgenes in the mitochondrial genome (mtDNA-PCR-RFLP). A di-sadvantage of the mtDNA procedure is that restriction enzyme digestion of the PCR products is needed. More recently, Zijlstraet al.achieved rapid identification ofM.incognita,M.javanicaandM.arenariaby developing species-specific sequence characterized amplified region (SCAR) primers. However, SCAR-based PCR procedures for direct diagnosis of single juveniles of the threeMeloidogynespecies have not been reported. The present study constitutes a continued effort toward the development of SCAR primers for identification ofM.incognita,M.javanicaandM.arenaria. We sequenced several species-specific random amplified polymorphic DNA (RAPD) markers and screened for SCAR primers with high amplification specificity and efficiency. Our objective was to develop sens