3个野生毛花猕猴桃居群的SSR多样性分析

YANG Miaoxian, PAN Weiming, ZHOU Lingyan, LIU Wen, YE Chanjuan, LIU Shenghong, WAN Xiaorong, LIANG Hong. SSR Analysis and Genetic Diversity in Three Populations of Wild Actinidia eriantha from Heping County, Guangdong Province[J]. Journal of South China Normal University (Natural Science Edition), 2014, 46(4): 2. DOI: 10.6054/j.jscnun.2014.06.115

Citation: YANG Miaoxian, PAN Weiming, ZHOU Lingyan, LIU Wen, YE Chanjuan, LIU Shenghong, WAN Xiaorong, LIANG Hong. SSR Analysis and Genetic Diversity in Three Populations of Wild Actinidia eriantha from Heping County, Guangdong Province[J]. Journal of South China Normal University (Natural Science Edition), 2014, 46(4): 2. DOI: 10.6054/j.jscnun.2014.06.115

1.

1 仲恺农业工程学院生命科学学院,广州 510225;

2.

2华南农业大学农学院,广州 510642

详细信息

作者简介:

梁红，教授，Email: lhofice@163.com

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SSR Analysis and Genetic Diversity in Three Populations of Wild Actinidia eriantha from Heping County, Guangdong Province

1.

1College of Life Sciences,Zhongkai University of Agriculture and Engineering,Guangzhou 510225;

2.

2 College of Agriculture,South China Agricultural University,Guangzhou 510642

摘要

摘要: 利用9对SSR引物对来自和平县上陵镇高峰和羊石、上陵镇桃源村及热水镇3个居群的野生毛花猕猴桃不同植株叶DNA进行PCR扩增，以分析野生毛花猕猴桃居群遗传多态性.结果表明，3个野生毛花猕猴桃居群的遗传多态性百分比在97.3%~98.9%之间，平均多态性信息量 (polymorphism information content, 简称PIC值)在0.846~0.880之间，其中，上陵镇高峰和羊石野生毛花猕猴桃居群的SSR多样性相对较高，平均PIC值为0.880；而上陵镇桃源村野生毛花猕猴桃居群的SSR多样性较低，平均PIC值为0.846；但3个野生毛花猕猴桃居群的多态性百分比差异不明显.这些结果显示，广东省和平县不同野生毛花猕猴桃居群间遗传多样性较高，保存了丰富的猕猴桃野生居群基因资源.

Abstract: The genetic diversityin three wild A. eriantha populations which reside in Reshui town, Gaofeng and Yangshi villages of Shangling town, and Taoyuan village of Shangling town of Heping county, Guangdong Province,was analyzed in leaves DNA of different plants by PCR amplification with nine pairs of SSR primers. Present data showed that the percentages of polymorphic bands (PPB) of the three wild A. erianthapopulationswere between 97.3% and 98.9%, and the averagepolymorphism information content (PIC) in them werebetween 0.846 and 0.880. Among the three wild A. eriantha populations,the polymorphismof the populations inGaofeng and Yangshi of Shangling town was relatively high with an average PICof 0.880, and the polymorphismof the populations in Taoyuan village of Shangling town was relatively low with an average PICof 0.846. However, no significant difference of PPB was found in the three wild A. eriantha populations. These results indicate that there are still high genetic diversitiespreserved among the wildActinidia eriantha of different populations in Heping county of Guangdong Province, and rich gene resources could be utilizedfor kiwifruit breeding.

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目录

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