首页分享大花黄牡丹种子的质量评价及初步抗氧化活性研究

大花黄牡丹种子的质量评价及初步抗氧化活性研究

来源：花匠小妙招时间：2024-11-25 22:34

摘要：

结合一测多评（QAMS）和指纹图谱建立大花黄牡丹种子的质量评价方法，并对其抗氧化活性进行初步评价。采用Agilent DB-FATWAX柱（0.25 mm×30 m,0.25 μm），载气为高纯氮气，程序升温，检测器为氢火焰离子化检测器。以油酸甲酯为内参，所建相对校正因子重现性良好，采用t检验计算出QAMS与外标法所测含量结果的P值均大于0.05；不同产地大花黄牡丹样品指纹图谱与对照指纹图谱的相似度均大于0.99；表明QAMS、指纹图谱可用于大花黄牡丹种子的质量评价。主成分分析（PCA）结果显示，大花黄牡丹与牡丹样品数据点可区分为2类。通过DPPH和Fe3+还原法对比分析发现，大花黄牡丹种皮的自由基清除率和Fe3+还原能力要高于牡丹品种，而籽油的抗氧化能力则与牡丹无明显差异。本研究建立的测定分析方法准确、简便、可行。将一测多评结合指纹图谱可以有效评价不同产地大花黄牡丹种子的质量。大花黄牡丹种皮和种仁的抗氧化效果明显，可以用作食品、化妆品等新产品研发。

关键词: 大花黄牡丹, 一测多评, 指纹图谱, 质量评价, 抗氧化活性

Abstract:

A method for quality evaluation of Paeonia ludlowii seeds was established by combining QAMS and fingerprints,and its antioxidant activity was preliminarily evaluated.The analyses were performed on Agilent DB-FATWAX column (0.25 mm×30 m,0.25 μm),the carrier gas was high-purity nitrogen,temperature was programmed,and hydrogen flame ionization detector was used.Using methyl oleate as internal reference,the relative correction factor has good reproducibility.The P values of QAMS and external standard method are both >0.05 by t-test.The similarity between the fingerprints of P. ludlowii samples from different habitats and the control fingerprints was >0.99;The results showed that QAMS and fingerprints could be used for quality evaluation of P. ludlowii seeds.The results of principal component analysis (PCA) showed that the data points of P. ludlowii and P. suffruticosa could be divided into two categories.Through the comparative analysis of DPPH and Fe3+ reduction methods,it was found that the free radical scavenging rate and Fe3+ reduction capacity of the seed coat of P. ludlowii were higher than P. suffruticosa,while the antioxidant capacity of seed oil was not significantly different with P. suffruticosa.The analytical method established in this study is accurate,simple and feasible.One test and multiple evaluation combined with fingerprint can effectively evaluate the quality of P. ludlowii seeds from different habitats.The seed coat and kernel of P. ludlowii have obviously antioxidant effect,and can be used for research and development of new products such as food and cosmetics.

Key words: Paeonia ludlowii, QAMS, fingerprints, quality evaluation, antioxidant activity

中图分类号: R282.5