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大花蕙兰组织培养工厂化技术试验与示范推广.pdf

来源：花匠小妙招时间：2024-11-18 00:15

大花蕙兰组织培养工厂化生产技术试验与示范推广摘要大花葱兰是近年来我国花卉市场上十分流行的高档室内盆栽花卉，传统的繁殖方式不仅无法保持其品种特性，而且繁殖周期长、速度慢，繁殖系数低，远远不能满足大花葱兰商品化大生产的要求。通过不同外植体诱导培养基、切割方式、增殖分化培养基、生根培养基、光照条件等对大花葱兰组织培养的对比试验，提出了大花葱兰组织培养工厂化生产技术规程，在全国率先建立 “大花蕙兰组培快繁及工厂化生产技术体系”，使大花葱兰原球茎诱导率80%,增殖系数4.3，幼苗分化率达到76.7%，生根率为100%，组织培养苗移栽成活率为95%左右，商品苗成活率达98%以上，商品率85%以上，病虫防疫面积达 100%，检疫性病虫害为0，并应用于生产实践，生产大花葱兰优质组培苗245.5万株，年增殖率在1000倍以上，移栽成活率在98%以上，污染率在10%以下，完成我省大花葱兰组培和大规模生产上零的突破，开创了我国大花蕙兰组培苗生产的新里程。关键词:大花葱兰;组织培养;工厂化;试验;示范 NDIFLORUM TRIALSANDDEMONSTRATATIONSPREADOF TISSUECULTUREPRODUCETECHNOLOGIESINFACTORY ABSTRACT Thecymbidiumgrandiflorum,astopgradekindofflowersplantedinhouses,whichis muchmorepopularatflowermarketinChina.Thertaditionalreproductonmethodsdonot satisfytheneedofcommercialyieldultimately,sincethesemethodsnotonlycannotkeep thespeciesproperties,butthecycleislonger,thespeedisslowerandthereproductonrateis lowerinbreedingprocess.Accordingtocomparativeexperimentsexertedontissueculture ofcymbidiumgrandiflorum,suchasdifferentexplant,inducementsubsrtate,slicemanners, multiplicationdifferentiationsubstrate,rootagesubsrtateandsunlightconditions,etc.,the writerputforwordaseriesofregulationsontissuecultureproducetechnologiesinfactory withregardtotheflower,andtaketheleadincreatingtissueculture,quickreproduction andproduedinfactorysystemofcymbidiumgrandiflorum.Theresultsofthesystemis thattheprotocorminducerateofcymbidiumgrandiflorumis80%,themultiplicationrateis 4.3,therateofyoungplantsdifferentiationreachto76.7%;therateofrootageis100%,the survivalrateofinfanciesintissueculturetransplantisaround95%,thesurvivalrateof commercialplantsisabove98%,thecommodiytrateishigherthan85%,thediseasesand insectspreventioncoveragerateis100%,thequarantinedplantdiseasesandinsectpestsis zero.Whenthenumberof2455000highqualiytplantsoftissuecultureareproduced,the annualmultiplicationrateismorethan1000times,thesurvivalrateoftransplant