首页分享火棘花挥发油化学成分的GC

火棘花挥发油化学成分的GC

来源：花匠小妙招时间：2024-10-30 16:06

摘要： 采用水蒸气蒸馏法提取火棘花挥发油，利用气相色谱—质谱联用技术（GC-MS）分析其化学成分。使用维生素C和BHT为阳性对照，以DPPH自由基、亚硝酸钠清除作用为指标评价挥发油的抗氧化活性。从挥发油中鉴定了77个化合物，占挥发油总量的83.77%，含有多种生物活性成分，以萜类及其含氧衍生物（50.31%）、烷烃（18.52%）、醛（5.54%）为主；挥发油对DPPH自由基、亚硝酸钠有明显的清除作用，清除率为50%时，其体积分别为43.51、79.48 μL，样品量与清除率间呈量效关系；挥发油对DPPH自由基的清除效果略低于1 mg·mL-1维生素C，对亚硝酸钠的清除效果优于1 mg·mL-1的BHT。

关键词: 火棘花, 挥发油, 化学成分, GC-MS, 抗氧化活性

Abstract: The volatile oil from Pyracantha fortuneana flowers was extracted by watersteam distillation and analyzed by GC-MS. The antioxidant activity of volatile oil was evaluated by DPPH·and sodium nitrite scavenging capacity with VC and BHT as the positive controls. Seventy-seven compounds, which occupied 83.77% of total constituents and contained many bioactive constituents, were identified. Terpenes and their derivatives (50.31%), alkanes (18.52%), and aldehydes (5.54%) were found to be dominant components in the volatile oil. The volatile oil exhibited significant scavenging capacity against DPPH·and sodium nitrite in a concentration-dependent fashion. When the scavenging rate against DPPH· and sodium nitrite of volatile oil was 50%, the volumes of volatile oil were 43.51 and 79.48 μL, respectively. The scavenging capacity against DPPH· of volatile oil was slightly worse than that of 1 mg·mL-1 Vc and the scavenging capacity against sodium nitrite of volatile oil was better than that of 1 mg·mL-1 BHT.

Key words: Pyracantha fortuneana flowers, volatile oil, chemical constituents, GC-MS, antioxidant activity

中图分类号:

Q946.89