首页分享 TRPA5，一种锚蛋白亚家族昆虫瞬时受体电位通道，以多种剪接变体形式在苹果蠹蛾（鳞翅目：卷蛾科）的触角中表达。

TRPA5，一种锚蛋白亚家族昆虫瞬时受体电位通道，以多种剪接变体形式在苹果蠹蛾（鳞翅目：卷蛾科）的触角中表达。

来源：花匠小妙招时间：2026-02-06 07:39

Abstract

Transient receptor potential (TRP) channels are an ancient family of cation channels, working as metabotropic triggers, which respond to physical and chemical environmental cues. Perception of chemical signals mediate reproductive behaviors and is therefore an important target for sustainable management tactics against the codling moth Cydia pomonella L. (Lepidoptera: Tortricidae). However, olfactory behavior strongly depends on diel periodicity and correlation of chemical with physical cues, like temperature, and physical cues thus essentially contribute to the generation of behavioral response. From an antennal transcriptome generated by next generation sequencing, we characterized five candidate TRPs in the codling moth. The coding DNA sequence of one of these was extended to full length, and phylogenetic investigation revealed it to be orthologous of the TRPA5 genes, reported in several insect genomes as members of the insect TRPA group with unknown function but closely related to the thermal sensor pyrexia Reverse transcription PCR revealed the existence of five alternate splice forms of CpTRPA5. Identification of a novel TRPA and its splice forms in codling moth antennae open for investigation of their possible sensory roles and implications in behavioral responses related to olfaction.

摘要

瞬时受体电位（TRP）通道是一类古老的阳离子通道家族，作为代谢型触发器，对物理和化学环境线索做出反应。化学信号的感知介导生殖行为，因此是针对苹果蠹蛾Cydia pomonella L.（鳞翅目：卷蛾科）的可持续管理策略的重要目标。然而，嗅觉行为强烈依赖于昼夜节律以及化学信号与物理线索（如温度）的相关性，因此物理线索对行为反应的产生起着重要作用。通过下一代测序产生的触角转录组，我们在苹果蠹蛾中鉴定了五个候选TRP。其中一个的编码DNA序列被扩展到全长，系统发育研究表明它与TRPA5基因直系同源，在几个昆虫基因组中，该基因作为昆虫TRPA组的成员，功能未知，但与热传感器发热密切相关。逆转录PCR揭示了CpTRPA5存在五种可变剪接形式。在苹果蠹蛾触角中鉴定出一种新型TRPA及其剪接形式，为研究它们在嗅觉相关行为反应中的可能感觉作用和影响开辟了道路。