首页分享苜蓿银纹夜蛾核多角体病毒与Bt混合试剂对葡萄花翅小卷蛾的毒力分析

苜蓿银纹夜蛾核多角体病毒与Bt混合试剂对葡萄花翅小卷蛾的毒力分析

来源：花匠小妙招时间：2026-02-06 07:39

摘要： 为测定野生型和重组型苜蓿银纹夜蛾核型多角体病毒（Autographa californica multiple nucleopolyhedrovirus，AcMNPV）在葡萄花翅小卷蛾生物防治中的应用潜力，本文以野生型和重组型AcMNPV及其与苏云金杆菌（Bt）的混合试剂为试验材料，分别测定病毒及其与Bt的混合试剂对葡萄花翅小卷蛾幼虫的室内生物活性。研究结果表明：感染WT（野生型AcMNPV）、WT-31（AcMNPV携带苹果蠹蛾颗粒体病毒Cydia pomonella granulovirus (CpGV) orf31）、WTgp64-31（AcMNPV缺失gp64基因、携带CpGV orf31）的幼虫逐日死亡率呈现逐渐上升趋势，但作用速率缓慢；WT与WT-31的校正死亡率分别为80.36%、71.43%，并显著高于WTgp64-31（58.93%），但前两者之间差异不显著性。以WT和WT-31为基础，在混合有1000 IU/mL Bt的情况下，进一步分析表明，WT-31的LC50和LC90分别为1.66×103 PIB/mL、3.07×105 PIB/mL；浓度为104PIB/mL、105 PIB/mL的WT-31的LT50分别为5.67 d、4.24 d，LT90分别为11.62 d、9.33 d；AcMNPV能够感染葡萄花翅小卷蛾，较低浓度的Bt能够显著促进重组AcMNPV（WT-31）的杀虫效率。研究结果将为葡萄花翅小卷蛾的生物防治提供理论依据。

关键词: 葡萄花翅小卷蛾, 苜蓿银纹夜蛾核多角体病毒, 苹果蠹蛾颗粒体病毒, 苏云金杆菌

Abstract: The potential of wild-type and recombinant Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and their mixtures with Bacillus thuringiensis (Bt) in the biological control of Lobesia botrana larvae were determined. The daily mortality rates of larvae infected with WT (wild type AcMNPV), WT-31 (AcMNPV carrying Cydia pomonella granulovirus (CpGV) orf31) and WTgp64-31 (AcMNPV lacking gp64 gene and carrying CpGV orf31) showed a gradual but slow increase. The corrected mortality rates with WT (80.36 %) and WT-31 (71.43%) were not significantly different, but were significantly higher than that with WTgp64-31 (58.93%). The LC50and LC90 of WT-31 mixed with 1000 IU/mL Bt were 1.66×103 PIB/mL and 3.07×105 PIB/mL, respectively. The LT50 of WT-31 at 104 PIB/mL and 105 PIB/mL was 5.67 d and 4.24 d, respectively, and the LT90 was 11.62 d and 9.33 d, respectively. The results show that AcMNPV can infect L. botrana and lower concentration of Bt can significantly promote the insecticidal efficiency of recombinant AcMNPV (WT-31), which serves a theoretical basis for the biological control of L. botrana.

Key words: Lobesia botrana, AcMNPV, CpGV, Bt

中图分类号:

S476.1