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基于叶片解剖结构的砂生槐群体抗旱性评价

来源：花匠小妙招时间：2024-10-11 02:19

摘要： 选取来自西藏不同地区11个群体的砂生槐作为研究材料，采用石蜡切片技术，测定11项叶片解剖结构指标，应用SPSS软件进行方差分析、相关性分析和聚类分析，对叶片解剖结构指标进行筛选，运用隶属函数法对不同群体的砂生槐进行综合评价。结果表明：（1）砂生槐属于典型的等面叶，各群体砂生槐的叶肉组织中都存在有海绵组织，海绵组织位于上下栅栏组织之间。不同群体砂生槐的解剖结构指标中，叶片厚度、栅栏组织厚度、主脉维管束直径和紧密度等差异达到极显著水平（P<0.01），栅海比差异达到显著水平（P<0.05），其它指标均未达到显著性水平。（2）通过数据分析，得到如下结果：叶片厚度、上表皮厚度和上表皮角质层厚度可以作为分析抗旱性的主要指标，而其它指标可以作为辅助指标。（3）群体LS1、NY1抗旱性较强，而NC1与YL1抗旱性较弱。对于砂生槐来说，影响其抗旱性的主要解剖结构指标包括叶片厚度、上表皮厚度和上表皮角质层厚度，这几项指标可以基本上反映出不同群体砂生槐抗旱性的一些差异；但是，如果要准确评价砂生槐的抗旱性，还需要考虑砂生槐种子萌发和幼苗期间的生理生化指标等来综合评价不同群体砂生槐的抗旱性。

关键词: 砂生槐, 群体, 叶片解剖结构, 抗旱性

Abstract: With Sophora moorcroftiana samples collected from 11 populations in Tibet, we processed them with paraffin section methods and measured 11 leaf anatomical structure indexes. Variance analysis, correlation analysis and hierarchical cluster analysis by SPSS were performed to screen for the most representative leaf anatomical structure indexes. The S.moorcroftiana from different population was evaluated by subordinate function. The Results showed:(1)S.moorcroftiana leaves were typical isolateral leaf in which spongy tissue exists in the middle of palisade tissue. Among the 11 populations, the thickness of leaf, the thickness of palisade tissue, the diameter of vein vascular bundle, and the tightness of cell structure showed extremely significant difference(P<0.01), P/S ratio showed significant difference(P<0.05), while other indexes showed no significant difference; (2)From data analysis, the thickness of leaf, upper epidermis and upper cuticle could be used as main drought analyze resistance index, other indexes can be used as subsidiary Index; (3)LS1 and NY1 populations showed stronger drought resistance than NC1 and YL1 populations. For S.moorcroftiana, the thickness of leaf, upper epidermis and upper cuticle are the main anatomical factors that affect the drought resistance and can be used as major indexes for the evaluation of drought resistance. However, other factors, including physiological and biochemical parameters in the period of seed germination and seedling, should be taken into consideration for more accurate and comprehensive evaluation of drought resistance in S.moorcroftiana.

Key words: Sophora moorcroftiana, population, leaf anatomical structure, drought resistance

中图分类号:

S792.26