赤松纯林不同松枯梢病病级针叶的内生真菌多样性及群落结构

摘要：

目的: 研究赤松纯林中不同枯梢病病级的赤松针叶内生真菌多样性及群落结构，分析病—非病针叶内生真菌的多样性以及群落结构差异，为赤松纯林经营提供依据。方法: 采用高通量测序技术对真菌ITS1区进行测序，利用生物信息学方法分析赤松纯林中赤松无病斑和不同染病程度的针叶内生真菌的多样性和群落结构。结果: 在赤松纯林中，赤松无病斑针叶内生真菌的多样性高于染病针叶的内部真菌多样性；无病斑针叶中内生真菌的优势菌为多臂菌属和暗色球菌属，在枯梢病菌的侵染后群落结构发生改变；主坐标分析结果显示，无病斑针叶内生真菌与受侵染的针叶内真菌的群落构成差异显著；染病较轻的针叶与无病斑针叶相比，共生营养型真菌占比下降，病理营养型真菌占比增高，而腐生营养型真菌在全部针叶样本均占有较高的比例（37.15%~65.87%）。结论: 在赤松纯林中，赤松针叶内生真菌的多样性及群落结构受到枯梢病侵染的影响，无病斑针叶与染病针叶内生真菌群落结构差异显著。真菌营养型分类证实腐生营养型真菌和共生营养型真菌在无病斑针叶中为主要真菌，而在染病较轻针叶中以病理营养型真菌为主。

关键词: 赤松, 松枯梢病, 内生真菌, 群落结构

Abstract:

Objective: The diversity and community structure of endophytic fungi in the needles of Pinus densiflora with different incidences of Sphaeropsis sapinea were studied, and the differences of endophytic fungal diversity and community structure was analyzed, so as to provide basis for the management of pure forests of P. densiflora. Method: High-throughput sequencing technology was used to sequence the ITS1 region of the fungal rRNA gene, and further the diversity and community structure of endophytic fungi in different level of disease of P. densiflora were analyzed by using bioinformatics technology. Result: In the pure pine stands, the diversity of endophytic fungi in the asymptomatic needles of P. densiflora was higher than that of infected needles. The dominant fungi in the asymptomatic needles were Trichomerium and Phaeococomyces. The community structure changed after the infection of the pathogens. The principal coordinate analysis showed that the clustering distance within the asymptomatic endophytic fungi or the infected endophytic fungi was closer than that between the asymptomatic and the infected endophytic fungi. Compared with the asymptomatic needles, the proportion of symbiotroph fungi decreased, and the proportion of pathotrophic fungi was increased, and the proportion of saprotroph fungi accounted for a higher proportion (37.15% -65.87%) in all needles. Conclusion: In pure pine forest, the diversity and community structure of the endophytic fungi of P. densiflora are affected by S. sapinea, and the difference in the structure of endemic fungi between symptomatic needles and asymptomatic needles is significant. Fungal trophic classification confirms that saprotroph fungi and symbiotroph fungi are the main fungi in asymptomatic conifers, while pathotroph fungi are the dominant in lighter infected needles.

Key words: Pinus densiflora, Sphaeropsis sapinea, endophytic fungi, community structure

中图分类号: 

S718

相关知识

桉树红叶枯梢病防治试验初报
Illumina高通量测序分析百合内生真菌群落多样性
抗病和感病香蕉品种根系内生细菌群落结构与多样性
解析桉树红叶枯梢病病因及防治方法
桂花内生真菌的多样性与群落结构
榕树系统发育及叶片性状对叶内生真菌群落结构和多样性的影响
抗感白粉病青稞的内生真菌群落差异分析
园林科普|雪松落针、干枯、发黄，警惕枯梢病危害!
烟草根黑腐病不同发病程度与土壤养分及微生物群落的关系
毛花猕猴桃可培养内生真菌菌群结构、多样性及生物活性分析

网址: 赤松纯林不同松枯梢病病级针叶的内生真菌多样性及群落结构 https://www.huajiangbk.com/newsview2390415.html