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植物病原真菌尖孢镰刀菌检测与定量研究进展

来源：花匠小妙招时间：2025-09-29 21:07

摘要： 尖孢镰刀菌（Fusarium oxysporum）是一种危害严重的土传病原真菌，被列为世界十大植物病原真菌之一。该菌能够侵染棉花（Gossypium hirsutum）、大豆（Glycine max）、西瓜（Citrullus lanatus）、香蕉（Musa nana）、番茄（Lycopersicon esculentum）和苜蓿（Medicago sativa）等100多种具有重要经济价值的作物，引起枯萎病和根腐病等。对土壤和植物根组织中的尖孢镰刀菌进行检测和定量是病害早期诊断和有效防治的基础。本文对国内外关于尖孢镰刀菌检测和定量方法（主要包括培养基菌落平板稀释法、常规PCR和实时荧光定量PCR等）及其应用进行总结，旨在对农牧业生产中尖孢镰刀菌检测和定量提供理论指导。

关键词: 尖孢镰刀菌, 检测, 定量, 实时荧光定量PCR

Abstract: Fusarium oxysporum is one serious soil-borne pathogenic fungus and is listed as one of the top ten plant fungal pathogens in the world. This fungus can infect more than 100 economically important crops such as cotton,soybean,watermelon,banana,tomato and alfalfa,causing wilt and root rot. The detection and quantification of F. oxysporum in soil and plant root tissues is the basis for early diagnosis and effective control of the disease. This article summarizes the research progress and application of F. oxysporum detection and quantification methods at domestic and overseas,mainly including medium colony plate dilution method,conventional PCR and real-time fluorescent quantitative PCR,etc. This review provides theoretical guidance for F. oxysporum detection and quantification in agriculture.

Key words: Fusarium oxysporum, Detection, Quantification, Real-time fluorescent quantitative PCR

中图分类号:

S432.4+4

网址: 植物病原真菌尖孢镰刀菌检测与定量研究进展 https://www.huajiangbk.com/newsview2388583.html

所属分类：花卉