首页分享壳聚糖果蔬保鲜复合涂膜的制备与保鲜效果研究进展

壳聚糖果蔬保鲜复合涂膜的制备与保鲜效果研究进展

来源：花匠小妙招时间：2025-09-25 08:19

摘要: 壳聚糖涂膜可以控制果蔬的失重率，减少腐烂，达到保持贮藏品质和延长货架期的效果；但是，单纯的壳聚糖涂膜因其抗菌性能不甚理想，使得其在实际应用中受到限制，壳聚糖基复合涂膜逐渐成为研究热点。本文总结了壳聚糖-酸、壳聚糖-提取物、壳聚糖-精油、壳聚糖-纳米材料和其他类型的复合涂膜的制备方法，分别探讨了复合涂膜在果蔬保鲜上的应用效果，并分析了复合涂膜的抗菌性能、保鲜机制及其发展趋势，为其在果蔬保鲜方面的深入研究和应用提供参考。

关键词: 壳聚糖 / 涂膜 / 保鲜 / 果蔬 / 复合涂膜

Abstract: Chitosan-based composite coating could control the weight loss, reduce the decay, maintaining the storage quality and prolonging the shelf life of fruits and vegetables. In this review, the biological characteristics of chitosan, such as the safety, the film forming property and the antimicrobial activity were introduced. Moreover, we investigated the effective of keeping fresh on fruits and vegetables and the preparation of the composite coating including chitosan with essential oil coating, chitosan with organic acid coating, chitosan with nanomaterials and others. This paper discussed the antimicrobial activity, the keeping fresh mechanism and the development trend for these composite coating. This review could provide the consultation for its further research and application.

图 1 壳聚糖的结构[6]

图 2 壳聚糖纳米二氧化钛复合涂膜材料SEM观察照片

图 3 壳聚糖复合涂膜材料保鲜效果模拟图[6]

[1]

XING Y, XU Q, CHE Z, et al. Effects of chitosan-oil coating on blue mold disease and quality attributes of jujube fruits [J]. Food & Function, 2011, 2(8): 466-474. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=03ee8b585475c0a8c7c3925e62d49af9

[2]

XING Y, XU Q, JIANG L, et al. Effect of different coating materials on the biological characteristics and stability of microencapsulated Lactobacillus acidophilus [J]. RSC Advances, 2015, 5(29): 22825-22837. doi: 10.1039/C4RA13578D

[3]

DUAN J L, ZHANG S Y. Application of chitosan based coating in fruit and vegetable preservation: a review [J]. Journal of Food Processing & Technology, 2013, 4(5): 227. http://www.cabdirect.org/abstracts/20133254869.html

[4]

ROMANAZZI G, FELIZIANI E. Use of chitosan to control postharvest decay of temperate fruit: effectiveness and mechanisms of action [M]. [S.l.]: Elsevier INC, 2016: 155.

[5]

ABDOU E S, NAGY K S A, ELSABEE M Z. Extraction and characterization of chitin and chitosan from local sources [J]. Bioresource Technology, 2008, 99(5): 1359-1367. doi: 10.1016/j.biortech.2007.01.051

[6]

XING Y, XU Q, LI X, et al. Chitosan-Based Coating with Antimicrobial Agents: Preparation, Property, Mechanism, and Application Effectiveness on Fruits and Vegetables [J]. International Journal of Polymer Science, 2016, (5, article 277):1. http://d.old.wanfangdata.com.cn/Periodical/scgyxyxb201803001

[7]

SANTOS S T D N, AGUIAR J A A A, OLIVEIRA C V D, et al. Efficacy of the application of a coating composed of chitosan and Origanum vulgare L. essential oil to control Rhizopus stolonifer and Aspergillus niger in grapes (Vitis labrusca L.)[J]. Food Microbiology, 2012, 32(2): 345-353. doi: 10.1016/j.fm.2012.07.014

[8]

AIDER M. Chitosan application for active bio-based films production and potential in the food industry: review [J]. LWT-Food Science and Technology, 2010, 43(6): 837-842. doi: 10.1016/j.lwt.2010.01.021

[9] 刘敏, 赵浩, 范贵生, 等.壳聚糖-酪蛋白酸钠可食性抑菌膜结构表征及抑菌性的研究[J].食品研究与开发, 2017, 38 (2): 12-16. doi: 10.3969/j.issn.1005-6521.2017.02.003 [10] 胡林峰, 邢荣娥, 刘松, 等.壳聚糖衍生物及其抑菌活性研究进展[J].植物保护, 2015, 41(3): 1-6. doi: 10.3969/j.issn.0529-1542.2015.03.001 [11] 董泽义, 谭丽菊, 王江涛.壳聚糖保鲜膜研究进展[J].食品与发酵工业, 2014, 40(6):147-151. http://d.old.wanfangdata.com.cn/Periodical/gfztb200406009 [12]

MOHAMMADI A, HASHEMI M, HOSSEINI S M. Chitosan nanoparticles loaded with Cinnamomum zeylanicum essential oil enhance the shelf life of cucumber during cold storage [J]. Postharvest Biology & Technology, 2015, 110(C): 203-213. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=8f8e575a6cc41c9320068e0dc3af02ae

[13]

XING Y, LI X, XU Q, et al. Effects of chitosan coating enriched with cinnamon oil on qualitative properties of sweet pepper (Capsicum annuum L.) [J]. Food Chemistry, 2011, 124(4): 1443-1450. doi: 10.1016/j.foodchem.2010.07.105

[14]

CAI J, WANG C, HV Y, et al. Structural characterization and antimicrobial activity of chitosan (CS-40)/nisin complexes [J]. Journal of Applied Polymer Science, 2010, 116(6): 3702-3707. doi: 10.1002/app.31936/full

[15]

MARTINEZ L R, MIHU M R, TAR M, et al. Demonstration of antibiofilm and antifungal efficacy of chitosan against candidal biofilms, using an in vivo central venous catheter model [J]. Journal of Infectious Diseases, 2010, 201(9): 1436-1440. doi: 10.1086/651175

[16]

SÁNCHEZGONZÁLEZ L, CHÁFER M, CHIRALT A, et al. Physical properties of edible chitosan films containing bergamot essential oil and their inhibitory action on Penicillium italicum [J]. Carbohydrate Polymers, 2010, 82(2): 277-283. doi: 10.1016/j.carbpol.2010.04.047

[17]

MA Q, ZHANG Y, CRITZER F, et al. Quality attributes and microbial survival on whole cantaloupes with antimicrobial coatings containing chitosan, lauric arginate, cinnamon oil and ethylenediaminetetraacetic acid [J]. International Journal of Food Microbiology, 2016, 235: 103-108. http://www.sciencedirect.com/science/article/pii/S0168160516303889

[18]

KAYA M, ESONIENE L, DAUBARAS R, et al. Chitosan coating of red kiwifruit (Actinidia melanandra) for extending of the shelf life [J]. International Journal of Biological Macromolecules, 2016, 85: 355-360. doi: 10.1016/j.ijbiomac.2016.01.012

[19]

PERDONES A, SÁNCHEZGONZÁLEZ L, CHIRALT A, et al. Effect of chitosan-lemon essential oil coatings on storage-keeping quality of strawberry [J]. Postharvest Biology & Technology, 2012, 70: 32-41. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=4f8cc036712cf38d73612d3dd6c6f3b3

[20]

VÁSCONEZ M B, FLORES S K, CAMPOS C A, et al. Antimicrobial activity and physical properties of chitosan-tapioca starch based edible films and coatings [J]. Food Research International, 2009, 42(7): 762-769. doi: 10.1016/j.foodres.2009.02.026

[21]

OCHOA-VELASCO C E, GUERRERO-BELTRÁN J Á. Postharvest quality of peeled prickly pear fruit treated with acetic acid and chitosan [J]. Postharvest Biology & Technology, 2014, 92(3): 139-145. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=1e53bdedab7d0c741bc1674bec083573

[22]

SUN D, LIANG G, XIE J, et al. Improved preservation effects of litchi fruit by combining chitosan coating with ascorbic acid treatment during postharvest storage [J]. African Journal of Biotechnology, 2010, 9(22): 3272-3279. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=Open J-Gate000001205768

[23]

XU Q, XING Y, CHE Z, et al. Effect of chtiosan coating and oil fumigation on the microbiological and quality safety of fresh-cut pear [J]. Journal of Food Safety, 2013, 33(2): 179-189. doi: 10.1111/jfs.2013.33.issue-2

[24]

LIU K, YUAN C, CHEN Y, et al. Combined effects of ascorbic acid and chitosan on the quality maintenance and shelf life of plums [J]. Scientia Horticulturae, 2014, 176(176): 45-53. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=c5852bfe8edf7e748d8a6d6be551d684

[25]

XING Y, LI X, XU Q, et al. Effects of chitosan-based coating and modified atmosphere packaging(map) on browning and shelf life of fresh-cut lotus root (Nelumbo nucifera Gaerth) [J]. Innovative Food Science & Emerging Technologies, 2010, 11(4): 684-689. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=1118a8f8b77cf57ae35928232544773f

[26]

PUSHKALA R, PARVATHY K R, SRIVIDYA N. Chitosan powder coating, a novel simple technique for enhancement of shelf life quality of carrot shreds stored in macro perforated LDPE packs [J]. Innovative Food Science & Emerging Technologies, 2012, 16(39): 11-20. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=012e315a439d454247bd043df3ada882

[27]

ZHANG Y, ZHANG M, YANG H. Postharvest chitosan-g-salicylic acid application alleviates chilling injury and preserves cucumber fruit quality during cold storage [J]. Food Chemistry, 2015, 174: 558-563. doi: 10.1016/j.foodchem.2014.11.106

[28] 李海燕, 刘桂萍, 刘蕾.壳聚糖复合涂膜常温保鲜草莓的研究[J].食品工业科技, 2012, 33(1) : 359-362. http://d.old.wanfangdata.com.cn/Periodical/spgykj201201120 [29] 贾小丽, 张平, 马涛, 等.壳聚糖涂膜对冬枣室温条件下生理生化的影响[J].保鲜与加工, 2006(1) : 25-27. doi: 10.3969/j.issn.1009-6221.2006.01.012 [30] 丘苑新, 曾晓房, 高苏娟, 等.壳聚糖涂膜处理对紫金春甜桔采后保鲜效果的影响[J].食品工业科技, 2013, 3(49): 328-331. http://d.old.wanfangdata.com.cn/Periodical/spgykj201309079 [31] 谢春晖.壳聚糖涂膜对冬枣和甜樱桃保鲜效果的研究[D].泰安: 山东农业大学, 2009. http://www.wanfangdata.com.cn/details/detail.do?_type=degree&id=Y1539367 [32] 李慧妍, 秦文, 辛松林, 等.壳聚糖涂膜保鲜对黄秋葵果实常温贮藏品质及生理的影响[J].食品工业科技, 2015, 36(19): 327-331. http://d.old.wanfangdata.com.cn/Periodical/spgykj201519069 [33] 腾维, 何彬语, 田亚龙, 等.酒石酸改性壳聚糖涂膜保鲜剂的制备与应用研究[J].广州化工, 2016, 44 (17): 103-105. doi: 10.3969/j.issn.1001-9677.2016.17.037 [34] 闫媛媛, 齐海萍, 郜玮, 等.壳聚糖与柠檬酸复配涂膜液对草莓保鲜效果的研究[J].大连民族学院学报, 2015, 17 (3): 224-227, 234. doi: 10.3969/j.issn.1009-315X.2015.03.008 [35] 赵宇瑛, 郑小林.壳聚糖涂膜对绿竹笋采后保鲜效果的影响[J].保鲜与加工, 2015, 15(3): 33-37. http://www.cnki.com.cn/Article/CJFDTOTAL-BXJG201503010.htm [36] 胡位荣, 刘顺枝, 黄惠娟, 等.抗坏血酸与壳聚糖处理对鲜切香芋贮藏效果的研究[J].食品科学, 2010, 31 (24): 436-439. http://d.old.wanfangdata.com.cn/Periodical/spkx201024095 [37]

KHALIFA I, BARAKAT H, EL-MANSY H A, et al. Enhancing the keeping quality of fresh straw berry using chitosan-incorporated olive processing wastes [J]. Food Bioscience, 2016, 13(1): 69. http://www.sciencedirect.com/science/article/pii/S2212429215300328

[38] 辛颖, 张华超, 薛伟.基于牛蒡提取液的壳聚糖膜改性研究[J].食品研究与开发, 2016, 37 (11): 40-45. doi: 10.3969/j.issn.1005-6521.2016.11.010 [39]

GUERRA I C, DE OLIVEIRA P D, DE A L, et al. Coatings comprising chitosan and Mentha piperita L. or Mentha villosa huds essential oils to prevent common postharvest mold infections and maintain the quality of cherry tomato fruit [J]. International Journal of Food Microbiology, 2015, 214: 168. doi: 10.1016/j.ijfoodmicro.2015.08.009

[40] 刘开华, 张宇航, 邢淑婕.茶多酚联合壳聚糖对黄瓜贮藏品质的影响[J].食品工业, 2012, 33(2) : 110. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK201200166754 [41] 陶永元, 舒康云, 吴加美, 等.茶多酚与壳聚糖复配对草莓保鲜效果的影响[J].中国食品添加剂, 2012, 112(5): 224-230. doi: 10.3969/j.issn.1006-2513.2012.05.030 [42] 魏佳, 马晓芬, 阿塔吾拉·铁木尔, 等.核桃青皮提取液与壳聚糖复合物对甜瓜采后品质的影响[J].食品科学, 2016, 37 (4): 242-248. http://d.old.wanfangdata.com.cn/Periodical/spkx201604044 [43] 张晶, 袁静, 李文香, 等.壳聚糖及甘草提取液对莱阳梨保鲜效果的影响[J].食品科学, 2011, 32 (18): 339-342. http://d.old.wanfangdata.com.cn/Periodical/dkj201516163 [44] 李湘利, 刘静, 魏子浩, 等.姜蒜浸提液与壳聚糖复合保鲜切分莲藕护色效果的研究[J].食品科技, 2010, 35(1): 253-256, 259. http://kns.cnki.net/KCMS/detail/detail.aspx?filename=SSPJ201001075&dbname=CJFD&dbcode=CJFQ [45] 陶永元, 舒康云, 蔡晨波, 等.八角等提取液与壳聚糖复合物对草莓保鲜效果的影响[J].中国食品添加剂, 2012 (3): 168-172. doi: 10.3969/j.issn.1006-2513.2012.03.028 [46]

ABDOLLAHI M, REZAEI M, FARZI G. A novel active bionanocomposite film incorporating rosemary essential oil and nanoclay into chitosan [J]. Journal of Food Engineering, 2012, 111(2): 343-350. doi: 10.1016/j.jfoodeng.2012.02.012

[47]

[48] 芮光伟, 邢亚阁, 许青莲, 等.壳聚糖丁香精油生物涂膜保鲜剂对圣女果品质的影响[J].西华大学学报(自然科学版), 2014, 33(5): 41-44. doi: 10.3969/j.issn.1673-159X.2014.05.010 [49] 邢亚阁, 许青莲, 车振明, 等.壳聚糖肉桂油生物保鲜剂对蒜薹贮藏品质的影响[J].西华大学学报(自然科学版), 2012, 31(6): 98-101, 112. http://scgx.chinajournal.net.cn/WKE/WebPublication/paperDigest.aspx?paperID=38A493AE-D099-4219-80B1-E75C9C8F3E64 [50] 张大凤, 邢亚阁, 许青莲, 等.壳聚糖生物涂膜保鲜剂配方优化及保鲜效果[J].西华大学学报(自然科学版), 2012, 31(3): 68-72. doi: 10.3969/j.issn.1673-159X.2012.03.018 [51]

ALVAREZ M V, PONCE A G, MOREIRA M D R. Antimicrobial efficiency of chitosan coating enriched with bioactive compounds to improve the safety of fresh cut broccoli [J]. LWT-Food Science and Technology, 2013, 50(1): 78-87. doi: 10.1016/j.lwt.2012.06.021

[52] 张文勇, 陈瑶, 任文琪, 等.柑橘精油和壳聚糖复合物对草莓保鲜效果的研究[J].山西化工, 2015, 35 (6): 22-24, 35. http://d.old.wanfangdata.com.cn/Periodical/shanxhg201506006 [53] 钟曼茜, 从心黎, 张史青, 等.黄皮精油-壳聚糖复合涂膜对番木瓜果实常温贮藏品质及生理的影响[J].食品工业科技, 2017, 38 (12): 297-301. http://d.old.wanfangdata.com.cn/Periodical/spgykj201712054 [54] 冯可, 胡文忠, 姜爱丽, 等.壳聚糖涂膜与牛至精油复配对鲜切菠萝的保鲜作用[J].食品安全质量检测学报, 2015, 6 (7): 2475-2481. http://d.old.wanfangdata.com.cn/Periodical/spaqzljcjs201507018 [55] 王磊明, 李洋, 张茜, 等.壳聚糖-肉桂精油复合膜对蓝莓保鲜效果的影响[J].食品科技, 2017, 42 (9): 14-21. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QKC20172017102000163553 [56]

SUN T, WU C, HAO H, et al. Preparation and preservation properties of the chitosan coatings modified with the in situ synthesized nano SiOx [J]. Food Hydrocolloids, 2016, 54(ISSN): 130-138. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=d5a8c32344e14da20f67aff923d97d8a

[57] 陈建中, 葛水莲, 李丹花, 等.纳米TiO2壳聚糖复方涂膜对草莓保鲜效果的研究[J].食品科技, 2016, 41(9): 65-70. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QKC20162016102000096289 [58]

SHI S, WANG W, LIU L, et al. Effect of chitosan/nano-silica coating on the physicochemical characteristics of longan fruit under ambient temperature [J]. Journal of Food Engineering, 2013, 118(1): 125-131. doi: 10.1016/j.jfoodeng.2013.03.029

[59]

SOUZA M P, VAZ A F M, CERQUEIRA M A, et al. Effect of an edible nanomultilayer coating by electrostatic self-assembly on the shelf life of fresh-cut mangoes [J]. Food & Bioprocess Technology, 2015, 8(3): 647-654. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=59cdaf11ff6e327bce3520d724ff102a

[60]

SONG H, YUAN W, JIN P, et al. Effects of chitosan/nano-silica on postharvest quality and antioxidant capacity of loquat fruit during cold storage [J]. Postharvest Biology & Technology, 2016, 119: 41-48. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=7710c55be555d541d870e0382100241a

[61] 陈镠, 余婷, 王允祥, 等.壳聚糖-纳米氧化锌复合涂膜对甜樱桃采后生理和贮藏品质的影响[J].核农学报, 2017, 31 (9): 1767-1774. http://d.old.wanfangdata.com.cn/Periodical/hnxb201709015 [62]

VELICKOVA E, WINKELHAUSEN E, KUZMANOVA S, et al. Impact of chitosan-beeswax edible coatings on the quality of fresh strawberries (fragaria ananassa cv camarosa) under commercial storage conditions [J]. LWT-Food Science and Technology, 2013, 52(2): 80-92. doi: 10.1016/j.lwt.2013.02.004

[63]

LI S, LIN J, CHEN X. Effect of chitosan molecular weight on the functional properties of chitosan-maltose Maillard reaction products and their application to fresh-cut Typha latifolia L [J]. Carbohydrate Polymers, 2014, 102(4): 682-690. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=c2cf1a226f5977f6ceff5bb32d799216

[64]

GAO P, ZHU Z, ZHANG P. Effects of chitosan-glucose complex coating on postharvest quality and shelf life of table grapes [J]. Carbohydrate Polymers, 2013, 95(1): 371-378. doi: 10.1016/j.carbpol.2013.03.029

[65]

WAEWTHONGRAK W, PISUCHPEN S, LEELASUPHAKUL W. Effect of Bacillus subtilis and chitosan applications on green mold (Penicilium digitatum Sacc.) decay in citrus fruit [J]. Postharvest Biology & Technology, 2015, 99: 44-49. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=e60da7bc0187b089aa25b7f21e154cc6

[66]

DURAN M, ADAY M S, ZORBA N N D, et al. Potential of antimicrobial active packaging 'containing natamycin, nisin, pomegranate and grape seed extract in chitosan coating? to extend shelf life of fresh strawberry [J]. Food & Bioproducts Processing, 2016, 98: 354-363. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=0d4765554c62cde97c6bd6594836b8b7

[67]

SAHA A, GUPTA R K, TYAGI Y K. Effects of edible coatings on the shelf life and quality of potato (Solanum tuberosum L.) tubers during storage [J]. Journal of Chemical & Pharmaceutical Research, 2014, 6(12): 802.

[68] 蒋红英, 吴淑清, 汪旭芳, 等.可食性膜对夏黑葡萄保鲜效果研究[J].中国酿造, 2012, 31(8): 106-109. doi: 10.3969/j.issn.0254-5071.2012.08.029 [69] 禄璐, 张曦燕, 李晓莺, 等.壳聚糖山梨酸钾复合涂膜对鲜果枸杞保鲜品质的影响[J].食品工业科技, 2017, 38 (9): 257-260. http://d.old.wanfangdata.com.cn/Periodical/spgykj201709052 [70] 王颖, 范春丽, 范芳.壳聚糖和氯化钙复合涂膜对杨桃的保鲜效果[J].河南农业科学, 2012, 41 (3): 125-128. doi: 10.3969/j.issn.1004-3268.2012.03.032 [71] 舒静, 陈季旺, 周琦, 等.壳聚糖-乳清分离蛋白复合膜对鲜切雷竹笋木质化和品质的影响[J].食品科学, 2014, 35 (10): 284-289. doi: 10.7506/spkx1002-6630-201410053 [72] 冯若瑶, 杨亚卉, 王衍鹏, 等.壳聚糖魔芋葡甘聚糖复合涂膜对白沙枇杷保鲜效果的影响[J].湖北农业科学, 2016, 55 (4): 1002-1004. http://d.old.wanfangdata.com.cn/Periodical/hbnykx201604046 [73] 姜燕, 柳佳齐, 尤婷婷, 等.壳聚糖-甲氧基果胶复合涂膜及冷藏对巨峰葡萄贮藏品质的影响[J].食品工业科技, 2014, 35 (21): 329-332, 336. http://d.old.wanfangdata.com.cn/Periodical/spgykj201421072 [74] 曹珍珍, 周林燕, 李淑荣, 等.壳聚糖和魔芋精粉复合涂膜对大白杏保鲜效果的影响[J].核农学报, 2014, 28 (11): 2051-2057. doi: 10.11869/j.issn.100-8551.2014.11.2051 [75]

KHALIFA I, BARAKAT H, EL-MANSY H A, et al. Improving the shelf-life stability of apple and strawberry fruits applying chitosan-incorporated olive oil processing residues coating [J]. Food Packaging & Shelf Life, 2016, 9: 10-19. http://www.sciencedirect.com/science/article/pii/S2214289416300461

[76]

ANDRES Y, GIRAUD L, GERENTE C, et al. Antibacterial effects of chitosan powder: mechanisms of action [J]. Environmental Technology Letters, 2007, 28(12): 1357-1363. doi: 10.1080/09593332808618893

[77]

BRAVOANAYA L M, SOLTERO J F, RINAUDO M. DNA/chitosan electrostatic complex [J]. International Journal of Biological Macromolecules, 2016, 88: 345-353. doi: 10.1016/j.ijbiomac.2016.03.035

[78]

LOU M M, ZHU B, MUHAMMAD I, et al. Antibacterial activity and mechanism of action of chitosan solutions against apricot fruit rot pathogen Burkholderia seminalis [J].Carbohydrate Research, 2011, 346(11): 1294-1301. doi: 10.1016/j.carres.2011.04.042

[79]

RISTIĆ T, LASIČ S, KOSALEC I, et al. The effect of chitosan nanoparticles onto Lactobacillus cells [J]. Reactive & Functional Polymers, 2015, 97: 56-62. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ae49235286985196ee7b7bdea440cbdb

[80]

LI J, WU Y, ZHAO L. Antibacterial activity and mechanism of chitosan with ultra highmolecular weight [J]. Carbohydrate Polymers, 2016, 148: 200-205. doi: 10.1016/j.carbpol.2016.04.025

[81]

MOHAMMADI A, HASHEMI M, HOSSEINI S M. Effect of chitosan molecular weight as micro and nanoparticles on antibacterial activity against some soft rot pathogenic bacteria [J]. LWT-Food Science and Technology, 2016, 71: 347-355. doi: 10.1016/j.lwt.2016.04.010

[82]

RAAFAT D, VON B K, HAAS A, et al. Insights into the mode of action of chitosan as an antibacterial compound [J]. Applied & Environmental Microbiology, 2008, 74(12): 3764-3773. http://d.old.wanfangdata.com.cn/OAPaper/oai_pubmedcentral.nih.gov_2446574

[83]

XING Y, LI X, ZHANG L, et al. Effect of TiO2 nanoparticles on the antibacterial and physical properties of polyethylene-based film [J]. Progress in Organic Coatings, 2012, 73(2/3): 219-224. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=0ed2bfcb271b6349fefc8b2c6d4d2e66

[84]

XING Y, XU Q, YANG S X, et al. Preservation mechanism of chitosan-based coating with cinnamon oil for fruits storage based on sensor data [J]. Sensors, 2016, 16(7): 111. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=sensors-16-01111