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蒲公英TmRAV1基因克隆及其响应脱落酸信号表达分析

来源：花匠小妙招时间：2025-09-02 07:29

2025年9月2日星期二

Gene cloning of TmRAV1 in Taraxacum mongolicum and analysis on its expression in response to abscisic acid signaling

摘要

根据脱落酸处理的蒲公英（Taraxacum mongolicum Hand.-Mazz.）的转录组数据，在蒲公英中克隆获得1个编码RAV转录因子的基因序列，命名为TmRAV1。TmRAV1的开放阅读框（ORF）长度为1 026 bp，编码342个氨基酸。TmRAV1的理论相对分子质量为38 229，理论等电点为pI 9.20。TmRAV1为不稳定蛋白，具有亲水性，没有跨膜结构域和信号肽，含有44个磷酸化位点。氨基酸序列比对结果显示：TmRAV1与莴苣（Lactuca sativa Linn.）LsRAV1氨基酸序列的同源性最高（一致性为85.88%），具有高度保守的AP2和B3结构域。系统发育分析结果表明TmRAV1与其他5种植物的RAV转录因子聚在一起。TmRAV1在蒲公英叶中的相对表达量显著（P<0.05）高于根和花。TmRAV1受100 μmol·L-1脱落酸和250 mmol·L-1 NaCl的显著诱导。TmRAV1能够显著上调脱落酸敏感型转录因子基因TmAREB1的表达。TmRAV1是核定位转录因子，与脱落酸信号核心蛋白家族成员TmSnRK2.6互作。综上所述，蒲公英TmRAV1响应脱落酸等多种信号，其编码蛋白与TmSnRK2.6互作，并调控TmAREB1的表达。

Abstract

Based on the transcriptome data of Taraxacum mongolicum Hand.-Mazz. treated with abscisic acid (ABA), a gene sequence encoding RAV transcription factor was cloned from T. mongolicum, and named as TmRAV1. The open reading frame. (ORF) of TmRAV1 is 1 026 bp in length, encoding 342 amino acids. The theoretical relative molecular mass of TmRAV1 is 38 229, and the theoretical isoelectric point is pI 9.20. TmRAV1 is an unstable protein with hydrophilicity, has no transmembrane domain and signal peptide, and contains 44 phosphorylation sites. The amino acid sequence alignment result shows that TmRAV1 has the highest homology with the amino acid sequence of LsRAV1 in Lactuca sativa Linn. (the identity is 85.88%), and has highly conserved AP2 and B3 domains. The phylogenetic analysis result indicates that TmRAV1 and RAV transcription factors in other five plants are clustered together. The expression level of TmRAV1 in leaf of T. mongolicum is significantly （P<0.05） higher than those in root and flower. TmRAV1 is significantly induced by 100 μmol·L-1 ABA and 250 mmol·L-1 NaCl. TmRAV1 can significantly up-regulate the expression of abscisic acid-sensitive transcription factor gene TmAREB1. TmRAV1 is a nucleus-localized transcription factor, and can interact with the abscisic acid signaling core protein family member TmSnRK2.6. In conclusion, TmRAV1 in T. mongolicum responds to multiple signals including abscisic acid, its encoding protein interacts with TmSnRK2.6, and regulates the expression of TmAREB1.

关键词蒲公英; TmRAV1; 基因克隆; 表达; 脱落酸信号响应Key wordsTaraxacum mongolicum Hand.-Mazz.; TmRAV1; gene cloning; expression; response to abscisic acid signaling作者吴志清1,2, 亓希武2, 房海灵2, 于盱2, 李莉2, 柏杨2, 刘群2, 梁呈元1,2所在单位1. 南京中医药大学，江苏南京 210023；2. 江苏省中国科学院植物研究所（南京中山植物园）江苏省植物资源研究与利用重点实验室, 江苏南京 210014点击量911下载次数740基金项目国家自然科学基金项目（32200299）; 江苏省自然科学基金项目（BK20220753）; 江苏省植物资源研究与利用重点实验室开放基金项目（JSPKLB202201; JSPKLB202310）