猫须草HPLC指纹图谱研究

2014,43(3): 197 ~ 201. Subtropical Plant Science 猫须草 HPLC 指纹图谱研究 李金雨，陈 淳，林建忠，胡维冀，许文江 （福建省亚热带植物研究所，福建省亚热带植物生理生化重点实验室，福建厦门 361006 ） 摘 要：为建立猫须草药材HPLC指纹图谱分析方法，采用高效液相色谱法，以Phenomenex Synergi 4u hydro-RP -1 250 ×4.60 mm为色谱柱，以甲醇-0.1% 甲酸溶液为流动相梯度洗脱，检测波长254 nm ，流速 1.0 mL·min ，柱 温 40 ℃。结果表明，建立的猫须草药材HPLC指纹图谱，确定了 15 个共有峰，各猫须草样品指纹图谱与对照 指纹图谱的相似度均在 0.9 以上。该方法简单、准确、重复性好，为更好地控制猫须草药材质量提供有效可靠 的方法。 关键词：猫须草；HPLC；指纹图谱 Doi: 10.3969/j.issn.1009-7791.2014.03.003 中图分类号：S567; R282.5 文献标识码：A 文章编号：1009-7791(2014)03-0197-05 Study on HPLC Fingerprint Analysis of Clerodendranthus spicatus LI Jin-yu, CHEN Chun, LIN Jian-zhong, HU Wei-ji, XU Wen-jiang （Fujian Key Laboratory of Subtropical Plant Physiology and Biochemistry, Fujian Institute of Subtropical Botany, Xiamen 361006, Fujian China) Abstract: To establish a method of fingerprint analysis on Clerodendranthus spicatus by HPLC, the chromatographic fingerprint was obtained by Phenomenex Synergi 4u hydro-RP 250 ×4.60 mm with the gradient elution solvent system composed of methanol-0.1% formic acid. The detective -1 wavelength was 254 nm; the flow rate was 1.0 mL·min and the column temperature was maintained at 40 ℃. The results showed that 15 co-possessing peaks were selected as the fingerprint peaks of C. spicatus and good similarities with correlation coefficients higher than 0.9 were found in fingerprints between the different samples of C. spicatus and the standard fi

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