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樱桃叶黄酮的体外抗氧化活性.pdf

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樱桃叶黄酮的体外抗氧化活性.pdf

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关键词：樱桃黄酮体外氧化活性资源描述：

研究论文
樱桃叶黄酮的体外抗氧化活性
王垣芳,修金霞,赵峰,李祖成2,郑珊1
(1.滨州広学院药学院,东燜台264003:2.滨州広学院燜台附広院,山东?台264003
摘要:考察櫻叶酮的体外抗氧他活性,为樱桃叶黄酮在医药、食品方面的应用提供理沧依
据。从樱桃叶中捉取黃酮,体外检测櫻桃叶黃酮对羟自由基(?OH)和超氧阴离子自由基(O2?)的
清除作用,以及对H2O2诱发小鼠红细胞氧化溶血和肝脂质过化反应的裨制作用。结果显示
桃叶黄酮对·OH和O2?具有清除作用,且呈现随登浓度依巍性,30g/mL的黄酮落液对·O
和O2·的清除率分別达到85.67%和72.45%,并且对?OH的清除能力优于维生素C;樱桃叶黄酮
对肝匀浆脂质过氧化反应和过氧化氯(H202)诱发红细胞氧化溶血具有抑制作用,当终质登浓度
为30g/mnl.时,抑辛分别为79.82%和75.54%。表明櫻桃叶责酮具有质最浓度依赖性的体外
抗氧化活性。
关键词:樱桃叶:黄酮;抗氧化活性;体外
中图分类号:R931.71;比963文献标志码:A文章编号:1673-1689(2014)09-0966-05
Antioxidant Activity of Flavonoids from Cherry Leaf in Vitro
WA V( Yuanyang,X/ linxia,∥ HA ene,∥ Mucheng2,∥Hん tr Shiat
1. Pharmacy College of Binzhou Medical University, Yantai 264003, China; 2. Y antai Affiliated Hospital of
Birhom Meaical University, Yanlai 264003Chira)
Abstract This stucly was undertaken to extract flavonoids from cherry leaves and to investigate the
anli-oxidalive aclivily in vilro.Tolal flavonoids were extracted from cherry leaves. The antioxidant
activities of the flavonoids were evaluated by the scavenging power on free radicals. OH and O
and inhibiting effects on erythro tes hemolysis and lipid peroxidation of hepatic homogenate in
itro system. The flavonoids showed coneentration -dependent scavenging power on.OH and O3
Thc seavcnging rales for OH and O2 - of 30 Mg/ML Flavonoids solulion were 85.67 and 72.45
respectively, and the scavenging eapacity for.OH was strongcr than Vitamin C. Cherry leaves
flavonoids also showed inhibition on lipid peroxidation of hepatic homogenate and on hydrogen
pcroxidc-indueed cry Lhrocyle hemolysis in a concenlralion-dependenl manner. The inhibition rale
were 79.82% and 75.54 in final concentration 30 Mg/ml. Cherry leaves flavonoids have
concentration-dependent antioxidant activity in vitro, which can e used as natural plant
anlioxidalls or food additive or Ihe developmenl and lilialio
收稿日期;21.3-12-11
基金项員;山东省自然科学基金项目(7R21IHIM59);刺台市科学技ド发尽计灯项目2009168);滨州庆学院大学生科技创新项目
/ RY20101KCX60
作者简介:垣芳(1963ー),女,山东烟台人,医学硕上,教授,主要从ヨ天然药物活性研究。I-mnil: hyytwyf(o163.rmn
9o6 Journal of Fwid Stience and Binethnlngy nl33 NG.9 2014+
万方数据