HPLC同时测定地黄中5种苷类成分的含量

摘要： 建立高效液相色谱法同时测定地黄中梓醇、地黄苷D、益母草苷、毛蕊花糖苷和异毛蕊花糖苷含量的方法。采用SynergiTM Hydro-RP 80色谱柱（250 mm × 4.6 mm，4 μm）；流动相为乙腈-0.2%磷酸水，梯度洗脱；流速为1 mL/min，柱温35 ℃；检测波长：梓醇、地黄苷D和益母草苷为203 nm；毛蕊花糖苷和异毛蕊花糖苷为334 nm。地黄中5种苷类成分分离良好，各成分含量与峰面积在测定范围内均呈良好的线性关系（r≥0.9990），加样回收率分别为96.43%、97.52%、96.16%、100.48%、97.21%，RSD分别为1.44%、2.56%、1.07%、1.98%、3.35%。该方法简单、快捷，重现性好，准确率高，可为地黄的质量控制研究提供依据。

关键词: 地黄, HPLC, 梓醇, 地黄苷D, 益母草苷, 毛蕊花糖苷, 异毛蕊花糖苷

Abstract: To establish an HPLC method for simultaneous determination of catalpol,rehmannioside D,leonuride,acteoside,isoacteoside in Rehmanniae Radix.SynergiTM Hydro-RP 80 column (250 mm× 4.6 mm,4 μm)was used,with acetonitrile-0.2% phosphoric acid as the mobile phase in gradient elution mode.The column temperature was 35 ℃,with flow rate1.0 mL/min.The UV detection wavelength was set at the maximum absorption wavelength,203 nm for catalpol,rehmannioside D and leonuride,334 nm for acteoside and isoacteoside.The five kinds of glycosides in Rehmanniae Radix were separated well,and the linear relation was obtained(r≥0.9990).The average recoveries were 96.43%,97.52%,96.16%,100.48%and 97.21%,with RSD 1.44%, 2.56%,1.07%,1.98%,3.35%,respectively.Themethod is simple,accurate,reproductive,and can provide the basis for the quality control of Rehmanniae Radix.

Key words: Rehmanniae Radix, HPLC, catalpol, rehmannioside D, leonuride, acteoside, isoacteoside

中图分类号: 

R284.1

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