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油菜花序轴、叶片组织培养中形态发生的细胞组织学研究

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文章导航 > 植物科学学报 > 1988 > 6(1) : 45-48.

周吉源, 程井辰, 王家训. 油菜花序轴、叶片组织培养中形态发生的细胞组织学研究[J]. 植物科学学报, 1988, 6(1): 45-48.

引用本文: 周吉源, 程井辰, 王家训. 油菜花序轴、叶片组织培养中形态发生的细胞组织学研究[J]. 植物科学学报, 1988, 6(1): 45-48.

Zhou Jiyuan, Cheng Jinchen, Wang Jiaxun. CYTOHISTOLOGICAL STUDIES ON THE MORPHOGENESIS OF RACHES AND LEAVES OF BRASSICA NAPUS L. IN TISSUE CULTURES[J]. Plant Science Journal, 1988, 6(1): 45-48.

Citation: Zhou Jiyuan, Cheng Jinchen, Wang Jiaxun. CYTOHISTOLOGICAL STUDIES ON THE MORPHOGENESIS OF RACHES AND LEAVES OF BRASSICA NAPUS L. IN TISSUE CULTURES[J]. Plant Science Journal, 1988, 6(1): 45-48.

油菜花序轴、叶片组织培养中形态发生的细胞组织学研究

华中师范大学生物系, 武汉

计量文章访问数: 02731 HTML全文浏览量: 0 PDF下载量: 01958 出版历程收稿日期: 1986-07-16 修回日期: 1899-12-31 发布日期: 1988-03-22

CYTOHISTOLOGICAL STUDIES ON THE MORPHOGENESIS OF RACHES AND LEAVES OF BRASSICA NAPUS L. IN TISSUE CULTURES

Department of Biology, Huazhong Normal University, Wuhan

摘要

摘要: 油菜花序轴、叶片在MS培养基上经诱导可产生愈伤组织并形成再生植株。愈伤组织形成可分3个时期:细胞启动期、分裂期和分化期。实验证明:花序轴启动部位多发生在皮层薄壁细胞、髓部薄壁细胞、韧皮薄壁细胞和木薄壁细胞。叶片主要是叶肉细胞、叶脉薄壁细胞和表皮细胞启动。细胞分裂期的特点是启动细胞反复增殖,形成连续的分生细胞层。细胞分化期的特点是一部分细胞保持分裂能力,一部分细胞分化形成薄壁细胞和输导分子。观察了愈伤组织中器官发生的部位和方式。实验证明,根的发生多为内起源,芽的发生多为外起源。

关键词: 形态发生 / 分化 / 油菜

Abstract: The calli were induced from the raehesand leaves of Brassiva napus L. on MS medium supplemented with 2,4-D, ADE, 6-BA. The plantlets were obtained after transferred the calli to MS medium with addition of 0.1-2mg/L NAA and 0.5-5mg/L 6-BA.The developmental stages of calli were observed i.e.cell activation, cell division and cell differentiation. The callus may be originated from epidermis, cortex parenchyma, pith parenchyma, phloem and xylem parenchyma of raches and mesophyll, branch veins parenchyma of leaf.Upon transferring the calli to the solid medium, two kinds of meristerustic tissues were formed——meristematic cellular mass and vascular nodule. The vascular nodule became a growth center without differentiation in early stage, but they were differentiated tracheids later in the inner surrounded by the cambium-like cells.The buds primordia are universally produced exogenously from meristematie cellular masses and the roots primordia are originated endogenously at random from either meristematic cellular mass or vascular nodule. Sometimes the roots formation from peripheral layer of callus were also observed.The relation between cell differentiation and tissue differentiation was discussed.

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计量文章访问数: HTML全文浏览量: 0 PDF下载量: 出版历程收稿日期: 1986-07-16 修回日期: 1899-12-31 发布日期: 1988-03-22