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广玉兰果实中双和厚朴酚A提取工艺研究

来源：花匠小妙招时间：2025-05-12 07:07

ZHANG Song-song, JIN Wei, LI Hong-mei, WU Cheng-zhu. Study on the extraction technology of bishonokiol A from Magnolia grandiflora seeds[J]. Journal of Bengbu Medical University, 2019, 44(1): 89-92. DOI: 10.13898/j.cnki.issn.1000-2200.2019.01.025

Citation: ZHANG Song-song, JIN Wei, LI Hong-mei, WU Cheng-zhu. Study on the extraction technology of bishonokiol A from Magnolia grandiflora seeds[J]. Journal of Bengbu Medical University, 2019, 44(1): 89-92. DOI: 10.13898/j.cnki.issn.1000-2200.2019.01.025

蚌埠医学院药学院, 安徽蚌埠 233030

详细信息

作者简介:

张松松(1988-), 男, 硕士研究生

通讯作者:

吴成柱, 博士, 硕士研究生导师, 副教授.E-mail:wuchengzhu0611@foxmail.com

中图分类号: R284.2

计量文章访问数: 04151 HTML全文浏览量: 02229 PDF下载量: 06 出版历程收稿日期: 2017-06-26 修回日期: 2018-06-08 刊出日期: 2019-01-14

Study on the extraction technology of bishonokiol A from Magnolia grandiflora seeds

School of Pharmacy, Bengbu Medical College, Bengbu Anhui 233030, China

摘要

摘要:

目的优化广玉兰果实的有效成分双和厚朴酚A的提取工艺。

方法采用反相高效液相色谱法测定不同提取溶媒中双和厚朴酚A的含量。以双和厚朴酚A得率为指标，采用L9（34）正交试验考察料液比、浸泡时间及提取次数3个因素对提取工艺的影响。

结果双和厚朴酚A的最佳提取工艺条件是正己烷为提取溶媒，料液比为130、浸泡时间为48 h、提取次数为3次。

结论通过正交设计法优选的双和厚朴酚A提取工艺简单、稳定可靠、重复性好。

Abstract:

ObjectiveTo optimize the extraction technology of bishonokiol A from the Magnolia grandiflora seeds.

MethodsThe concentration of bishonokiol A in different extracting solvents was determined using RP-HPLC method.The yield rate of bishonokiol A was set as the indictor, the effects of the ratio of material-to-liquid, soaking duration, and extraction times on the extraction technology were observed using L9(34) orthogonal test.

ResultsThe orthogonal test showed that the ratio of material-to-liquid for 130, soaking duration for 48 h, and extraction times for three times were the best technological condition of bishonokiol A extraction.

ConclusionsThe optimized extraction process designed by orthogonal test is simple, stable and reliable.

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图 1 广玉兰果实中双和厚朴酚A的HPLC色谱分析

A:正己烷提取浸膏; B:双和厚朴酚A对照品

表 1 试验因素计划表

水平因素料液比(A) 浸泡时间(B) 提取次数(C) 1 1:10 12 h 1 2 1:30 24 h 3 3 1:50 48 h 6

表 2 双和厚朴酚A标准品的精密度试验结果

测试序号峰面积 RSD值/% 1 72 361 120 0.68 2 71 059 594 3 71 897 281 4 72 152 289 5 72 902 056 6 72 217 752

表 3 用正己烷提取广玉兰果实粉末的重现性试验结果

测试序号峰面积 RSD值/% 1 1 161 111 1.60 2 1 180 453 3 1 197 080 4 1 194 462 5 1 185 337 6 1 218 410

表 4 广玉兰药材粉末正己烷提取浸膏的稳定性试验结果

测试时间峰面积 RSD值/% 0 2 948 182 1.39 2 3 012 384 4 3 024 136 6 3 062 223 8 3 006 213 10 3 023 739 24 3 078 221

表 5 各种溶媒提取浸膏的含量测定结果

方法药材量/g 浸膏量/g 进样浓度/(mg/mL) 双和厚朴酚A含量/(mg/g) 水提法 12.2332 2.7815 10.6 0.283 甲醛回流 12.1003 1.0083 10.3 1.553 甲醛浸泡 12.3401 0.8075 10.5 1.905 乙醇回流 12.3025 0.9069 10.2 2.059 乙醇浸泡 12.4545 0.4403 10.9 3.486 乙醇乙酯浸泡 20.0882 0.3515 12.1 3.291 二氯甲烷浸泡 19.9969 0.3679 11.7 3.991 正已烷浸泡 20.043 5 0.115 0 11.5 6.575

表 6 L9(34)正交试验设计

序号因素双和厚朴酚A含量/(mg/mL) A B C 1 1 1 1 0.869 2 1 2 2 0.789 3 1 3 3 0.887 4 2 1 3 0.844 5 2 2 1 0.841 6 2 3 2 0.912 7 3 1 2 0.811 8 3 2 3 0.498 9 3 3 1 0.772 K1 0.848 0.841 0.827 — K2 0.866 0.709 0.837 — K3 0.694 0.857 0.743 — R 0.172 0.148 0.094 —

表 7 正交试验方差分析表

变异来源 SS df MS F P 料液比 0.054 2 1.543 5.14 < 0.05 浸泡时间 0.040 2 1.143 5.14 < 0.05 提取次数 0.011 2 0.314 5.14 > 0.05 误差 0.110 6 — — —

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