中国石斛属植物遗传资源的AFLP分析

摘要：

中国是世界石斛属（Dendrobium）植物分布中心,其主要分布在云贵川等地区。采用扩增片段长度多态性（AFLP）分子标记方法,研究了石斛属92个种质的遗传多样性和亲缘关系。从24对引物组合中筛选出10对引物组合进行扩增,共扩增出2 329个位点,其中多态位点2 307个,占总扩增位点的99.04%。聚类分析将92个石斛种质,分为4个类群,种间遗传分化显著。AFLP分子标记将菱唇石斛和西畴石斛划为石斛组、金耳石斛划为寡花组,且研究结果表明,茎的形态和质地为石斛分类的重要性状,花的唇瓣和茎节的膨大非石斛分类的主要因素。

关键词: 兰科, 石斛属, AFLP, 扩增, 亲缘关系, 遗传多样性

Abstract:

Dendrobium plants are mainly distributed in Yunnan,Guizhou and Sichuan Province in China. China is the distribution center of Dendrobium plants in the world. In this study,Amplified Length Polymorphism（AFLP）was used to study the genetic diversity and phylogenetic relationship of 92 species of Dendrobium in China. Ten primer combinations were screened out from 24 pairs of primer combinations,and a total of 2 329 loci were amplified,among which 2 307 were polymorphic,accounting for 99.04% of the total loci. Phylogenetic analysis results showed that 92 Dendrobium samples were divided into four groups,and the genetic differentiation was significant. AFLP molecular markers supported that the two species of D. leptocladum and D. xichouense were classified into Sect. Dendrobium,and D. hookerianum was divided into Sect. Holochrysa. The results also showed that the shape and texture of the stem were important characteristics for the classification of Dendrobium,and the flower lip and enlargement of stem node were not the main factors for Dendrobium classification.

Key words: Orchidaceae, Dendrobium, AFLP, amplification, phylogenetic relationship, genetic diversity

中图分类号: 

S682.31

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