帝王花种子无菌播种和快速繁殖研究
, , , , , , , , 。 [ ] 。[ ] MS 1/2MS , , 、 。[ ]1/2MS+6-BA 0.01mg/L , 14 d ,35 d ;MS+6-BA 1.00 mg/L+IBA 0.01mg/L , 2.83 ;1/2MS+IAA 1.00 mg/L , 96%, 4.2 cm; ∶ =3∶1 , 85%。[ ] 。 www.taodocs.com , , , , , , , , 。 [ ] 。[ ] MS 1/2MS , , 、 。[ ]1/2MS+6-BA 0.01mg/L , 14 d ,35 d ;MS+6-BA 1.00 mg/L+IBA 0.01mg/L , 2.83 ;1/2MS+IAA 1.00 mg/L , 96%, 4.2 cm; ∶ =3∶1 , 85%。[ ] 。 www.taodocs.com
; ; ; S604 A 0517-6611(2020)02-0067-03 doi:10.3969/j.issn.0517-6611.2020.02.018 ( ) (OSID): Study on Aseptic Sowing and Rapid Propagation Technology for Protea cynaroides L.Seed CHEN Quan1,CHEN Hui-lan1,LI WEN-jian2 (1.Shanghai Sunqiao Modern Greenhouse Seed and Seedling Co.,Ltd.,Shanghai201210;2.Shanghai Sunqiao Yijia Agriculture Technology Co.,Ltd.,Shanghai201210) Abstract [Objective] To establish a system of aseptic sowing and rapid propagation technology for Protea cynaroides L..[Method]Different hormone combinations were added to MS or 1/2MS medium to screen the suitable induction,proliferation and rooting medium.[Result]The proper medium for the seeds induction of Protea cynaroides L.was 1/2MS medium containing 6-BA 0.01mg/L,seed germination was www.taodocs.com ; ; ; S604 A 0517-6611(2020)02-0067-03 doi:10.3969/j.issn.0517-6611.2020.02.018 ( ) (OSID): Study on Aseptic Sowing and Rapid Propagation Technology for Protea cynaroides L.Seed CHEN Quan1,CHEN Hui-lan1,LI WEN-jian2 (1.Shanghai Sunqiao Modern Greenhouse Seed and Seedling Co.,Ltd.,Shanghai201210;2.Shanghai Sunqiao Yijia Agriculture Technology Co.,Ltd.,Shanghai201210) Abstract [Objective] To establish a system of aseptic sowing and rapid propagation technology for Protea cynaroides L..[Method]Different hormone combinations were added to MS or 1/2MS medium to screen the suitable induction,proliferation and rooting medium.[Result]The proper medium for the seeds induction of Protea cynaroides L.was 1/2MS medium containing 6-BA 0.01mg/L,seed germination was www.taodocs.com
observed on 14 days,seedling formation was observed on 35 days; the most suitableproliferation medium was MS medium containing 6-BA 1.00 mg/L+IBA0.01mg/L with proliferation times 2.83;the best seedling rooting medium was 1/2MS medium containing IAA1.00 mg/L,rooting rate was up to 96%,plant length was up to 4.2 cm;seedlings were planted in substrate that composed with peat and perlite in a ratio of 3∶1,the survival percent of transplantation could reach 85%.[Conclusion]The study laid a foundation for rapid breeding of high quality seedling of Protea cynaroides L.. Key words Protea cynaroides L.; Aseptic sowing; Hormones ratio; Tissue culture (Protea cynaroides L.), , , , , , , 。 、 , [1- www.taodocs.com observed on 14 days,seedling formation was observed on 35 days; the most suitableproliferation medium was MS medium containing 6-BA 1.00 mg/L+IBA0.01mg/L with proliferation times 2.83;the best seedling rooting medium was 1/2MS medium containing IAA1.00 mg/L,rooting rate was up to 96%,plant length was up to 4.2 cm;seedlings were planted in substrate that composed with peat and perlite in a ratio of 3∶1,the survival percent of transplantation could reach 85%.[Conclusion]The study laid a foundation for rapid breeding of high quality seedling of Protea cynaroides L.. Key words Protea cynaroides L.; Aseptic sowing; Hormones ratio; Tissue culture (Protea cynaroides L.), , , , , , , 。 、 , [1- www.taodocs.com
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