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帝王花种子无菌播种和快速繁殖研究

来源:花匠小妙招 时间:2025-04-16 21:04

                         ,             ,        ,          ,            ,           ,             ,      ,       。     [  ]                  。[  ] MS 1/2MS      ,        ,       、        。[  ]1/2MS+6-BA 0.01mg/L             ,  14 d    ,35 d    ;MS+6-BA 1.00 mg/L+IBA 0.01mg/L          ,     2.83 ;1/2MS+IAA 1.00 mg/L        ,    96%,   4.2 cm;        ∶   =3∶1    ,       85%。[  ]                     。    www.taodocs.com                                ,             ,        ,          ,            ,           ,             ,      ,       。     [  ]                  。[  ] MS 1/2MS      ,        ,       、        。[  ]1/2MS+6-BA 0.01mg/L             ,  14 d    ,35 d    ;MS+6-BA 1.00 mg/L+IBA 0.01mg/L          ,     2.83 ;1/2MS+IAA 1.00 mg/L        ,    96%,   4.2 cm;        ∶   =3∶1    ,       85%。[  ]                     。    www.taodocs.com       

         ;    ;    ;             S604      A        0517-6611(2020)02-0067-03   doi:10.3969/j.issn.0517-6611.2020.02.018       (    )   (OSID):   Study on Aseptic Sowing and Rapid Propagation Technology for Protea cynaroides L.Seed   CHEN Quan1,CHEN Hui-lan1,LI WEN-jian2 (1.Shanghai Sunqiao Modern Greenhouse Seed and Seedling Co.,Ltd.,Shanghai201210;2.Shanghai Sunqiao Yijia Agriculture Technology Co.,Ltd.,Shanghai201210)   Abstract [Objective] To establish a system of aseptic sowing and rapid propagation technology for Protea cynaroides L..[Method]Different hormone combinations were added to MS or 1/2MS medium to screen the suitable induction,proliferation and rooting medium.[Result]The proper medium for the seeds induction of Protea cynaroides L.was 1/2MS medium containing 6-BA 0.01mg/L,seed germination was    www.taodocs.com                ;    ;    ;             S604      A        0517-6611(2020)02-0067-03   doi:10.3969/j.issn.0517-6611.2020.02.018       (    )   (OSID):   Study on Aseptic Sowing and Rapid Propagation Technology for Protea cynaroides L.Seed   CHEN Quan1,CHEN Hui-lan1,LI WEN-jian2 (1.Shanghai Sunqiao Modern Greenhouse Seed and Seedling Co.,Ltd.,Shanghai201210;2.Shanghai Sunqiao Yijia Agriculture Technology Co.,Ltd.,Shanghai201210)   Abstract [Objective] To establish a system of aseptic sowing and rapid propagation technology for Protea cynaroides L..[Method]Different hormone combinations were added to MS or 1/2MS medium to screen the suitable induction,proliferation and rooting medium.[Result]The proper medium for the seeds induction of Protea cynaroides L.was 1/2MS medium containing 6-BA 0.01mg/L,seed germination was    www.taodocs.com       

observed on 14 days,seedling formation was observed on 35 days; the most suitableproliferation medium was MS medium containing 6-BA 1.00 mg/L+IBA0.01mg/L with proliferation times 2.83;the best seedling rooting medium was 1/2MS medium containing IAA1.00 mg/L,rooting rate was up to 96%,plant length was up to 4.2 cm;seedlings were planted in substrate that composed with peat and perlite in a ratio of 3∶1,the survival percent of transplantation could reach 85%.[Conclusion]The study laid a foundation for rapid breeding of high quality seedling of Protea cynaroides L..   Key words Protea cynaroides L.; Aseptic sowing; Hormones ratio; Tissue culture      (Protea cynaroides L.),     ,         ,                 ,   ,      ,          ,           。             、         ,                [1-   www.taodocs.com       observed on 14 days,seedling formation was observed on 35 days; the most suitableproliferation medium was MS medium containing 6-BA 1.00 mg/L+IBA0.01mg/L with proliferation times 2.83;the best seedling rooting medium was 1/2MS medium containing IAA1.00 mg/L,rooting rate was up to 96%,plant length was up to 4.2 cm;seedlings were planted in substrate that composed with peat and perlite in a ratio of 3∶1,the survival percent of transplantation could reach 85%.[Conclusion]The study laid a foundation for rapid breeding of high quality seedling of Protea cynaroides L..   Key words Protea cynaroides L.; Aseptic sowing; Hormones ratio; Tissue culture      (Protea cynaroides L.),     ,         ,                 ,   ,      ,          ,           。             、         ,                [1-   www.taodocs.com       

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