首页分享应用拮抗和ISSR方法综合鉴定灰树花菌株

应用拮抗和ISSR方法综合鉴定灰树花菌株

来源：花匠小妙招时间：2025-03-12 21:10

摘要： [目的]准确、科学地鉴定灰树花菌株，明确其亲缘关系。[方法]采用拮抗和ISSR分子标记对供试的11个灰树花菌株进行鉴定和遗传多样性分析。[结果]拮抗反应表明，供试菌株之间拮抗作用有一定差异，根据拮抗反应的有无，供试菌株分为4组。ISSR分子标记分析表明，当相似性系数达到0.87水平时，供试菌株聚成5组，第1组包括菌株1（石家庄灰树花）、2（台灰）、10（XG-W）、5（Gr0001+2）、6（CN）、7（雪圆）；第2组为菌株11（CN-9）；第3组为菌株4（Gr0003）；第4组包括菌株8（H）、9（Hokto）；第5组为菌株3（Gr20）。[结论]拮抗反应与ISSR两种方法结论较一致，推测石家庄灰树花与台灰，Gr0001+2、CN与雪圆，H与Hokto这3组菌株分别来源于同一个菌株，属于同种异名现象Gr0003、H和Hokto亲缘关系较远；Gr20与其他菌株亲缘关系最远。

关键词: 拮抗, ISSR技术, 灰树花, 鉴定, 聚类分析

Abstract: [Objective]To reliably identify strains of Grifola frondosa and precisely determine the genetic relationship among them.[Method]The identification and genetic diversity analysis on 11 strains of G. frondosa were carried out by the combined use of the antagonistic challenging test and ISSR.[Result]The antagonistic test on the strains demonstrated apparent differences on their responses. Presence or absence of antagonism allowed the classification of the strains into 4 distinct groups. Meanwhile, from the ISSR analysis, when the similarity coefficient reached 0.87, the strains were clustered into 5 groups. They were Group 1 that included Strain #1 (Shijiazhuang ash tree flower), Strain #2 (stage ash), Strain #10 (XG-W), Strain #5 (Gr0001+2), Strain #6 (CN), and Strain #7 (snow circle); Group 2, Strain #11 (CN-9); Group 3, Strain #4 (Gr0003); Group 4, Strain #8 (H) and Strain #9 (Hokto); and, Group 5, Strain #3 (Gr20).[Conclusion]It appeared that Strain #1 and Strain #2 belonged to a same species; so were Strains #5, Strain #6 and Strain #7, and Strains #8 and Strain #9. At the similarity coefficient of 0.8, Strain #4 could be clustered with Strain #8 and Strain #9 with slight differences indicating a distance on genetic relationship among them. Strain #3 had a lowest coefficient of 0.34, therefore, it was most remotely related to all.

Key words: antagonistic effect, ISSR, Grifola frondosa, identification, cluster analysis

中图分类号:

S646