首页分享石韦提取物抗氧化及抑制亚硝化作用研究

石韦提取物抗氧化及抑制亚硝化作用研究

来源：花匠小妙招时间：2025-01-16 18:59

摘要：

研究石韦不同溶剂提取物的总酚、总黄酮含量和抗氧化及抑制亚硝化活性，并分析其相关性。采用福林酚比色法和硝酸铝络合分光光度法分别测定各提取物中总酚、总黄酮的含量，以DPPH法、ABTS法和普鲁士蓝法分别测定各提取物的自由基清除能力和铁离子还原力，采用盐酸萘乙二胺法和α-萘胺法分别测定石韦各提取物对亚硝酸盐的清除率和对亚硝胺合成的阻断率，采用Pearson 法分析总酚、总黄酮含量与抗氧化及抑制亚硝化活性的相关性。结果表明：石韦不同溶剂提取物的总酚和总黄酮含量存在显著性差异，其中正丁醇提取物的总酚、总黄酮含量均最高，分别为29.85±2.15和37.23±2.41 mg/g。各提取物均具有较强的抗氧化和抑制亚硝化活性，以正丁醇提取物的效果最显著，其DPPH自由基清除能力（IC50=44.14±1.21 μg/mL）、ABTS自由基清除能力（IC50=97.47±12.10 μg/mL）和还原能力（822.08±24.82 μmoL Vc/g），以及对亚硝酸盐的清除能力（IC50=7.071±0.231 mg/mL）、对亚硝胺合成的阻断能力（IC50=15.010±1.224 mg/mL）均最强，并显著强于其他提取物（P<0.05）。相关性研究显示，石韦各提取物的抗氧化活性和抑制亚硝化活性与其总酚和总黄酮含量呈显著正相关（P<0.05），表明总酚类和总黄酮类物质是石韦发挥作用的物质基础。通过对石韦不同提取物的总酚和总黄酮含量的测定、抗氧化和抑制亚硝化作用评价及相关性分析，为石韦的进一步开发利用提供实验数据和参考依据。

关键词: 石韦, 抗氧化, 抑制亚硝化, 总酚, 总黄酮

Abstract:

The total phenolic content,total flavonoid content,antioxidant activity,and nitrosation activity of different solvent extract from Pyrrosia lingua (Thunb.) Farwell were compared and analyzed for correlation.The contents of total phenolic and total flavonoid from P. lingua were determined by Folin-Ciocalteu colorimetric method and Al(NO3)3 complexation spectrophotometric method.DPPH,ABTS,and Prussian blue method were used to determine the free radical scavenging and iron reducing power of extracts from P. lingua.The removal rate of nitrite and the blocking rate of nitrosamine synthesis were determined by naphthylethylenediamine hydrochloride method and α-naphthylamine method,and Pearson correlation analysis method was used to measure their relationships.The results showed that there were significant differences in the contents of total phenolics and total flavonoids among different solvent extracts.The N-butyl alcohol phase contained the highest amount of total phenolics 29.85±2.15 mg/g and total flavonoids 37.23±2.41 mg/g.All the extracts showed strong antioxidant and nitrosation activity.The N-butyl alcohol phase also had the highest antioxidant activity and inhibitory activity among the four extracts (P<0.05),which showed DPPH radical scavenging activity(IC50=44.14±1.21 μg/mL),ABTS radical scavenging activity(IC50=97.47±12.10 μg/mL),reducing ferric(822.08±24.82 μmoL Vc/g),scavenging rate for nitrate(IC50=7.071±0.231 mg/mL) and blocking rate of nitrosamine(IC50=15.010±1.224 mg/mL),and were significantly stronger than those of other extracts(P<0.05).The results of correlation analysis showed that there were significant correlations between the contents of total phenolic and total flavonoids and antioxidant activity,inhibitory activity on nitrosation(P<0.05) of all extracts,and the material basis of antioxidant activity inhibitory activity on nitrosation of P. lingua may be phenolics and flavonoids.The evaluation of index components,activities,and related analysis of different solvent extract from P. lingua will provide experimental data for quality evaluation and rational utilization of P. lingua.

Key words: Pyrrosia lingua (Thunb.) Farwell, antioxidant, inhibition of nitrosation, total phenolics, total flavonoids

中图分类号: R932