首页分享维生素C和西兰花提取物治疗小鼠镉中毒的时间效应：睾丸组织和细胞凋亡指数

维生素C和西兰花提取物治疗小鼠镉中毒的时间效应：睾丸组织和细胞凋亡指数

来源：花匠小妙招时间：2025-01-12 00:23

摘要

重金属导致生殖系统疾病的增长率是不可否认的。时间和干扰化合物的影响也是至关重要的。本研究的主要目的是比较西兰花提取物和维生素C在镉中毒情况下对小鼠各种生殖参数的影响，特别关注时间的影响。将一百四十四只雄性小鼠随机分为六组。对照组（C）只接受水和标准饮食，没有任何干预措施。Cd组接受单次腹膜内剂量的氯化镉，剂量为1.5mg/kg。镉干预组以100mg/kg的剂量给予西兰花提取物（Cd + B100），200 mg/kg（Cd + B200）和300 mg/kg（Cd + B300）。此外，Cd + VC组采用镉和维生素C 200mg/kg腹膜内给药28天。在每周末（四个阶段），从每组中随机选择五只动物。对附睾精子进行精子参数分析，同时检查睾丸组织切片的组织学研究、细胞凋亡指数和氧化应激标志物。时间对镉对体重和睾丸增重的影响显著 + B300和Cd + VC组（p = 0.001）。除Cd外，所有组 + B100，生精细胞系和Johnson系数均显著高于Cd组（p = 0.001）。这些变化在Cd中尤为明显 + VC和Cd + B300组相对于时间（p

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The Effectiveness of Time in Treatment with Vitamin C and Broccoli Extract on Cadmium Poisoning in Mice: Histological Changes of Testicular Tissue and Cell Apoptotic Index.

The growth rate of reproductive system disorders caused by heavy metals is undeniable. The effect of time and interfering compounds are also of paramount importance. The main objective of this study was to compare the effects of broccoli extract and vitamin C in the context of cadmium poisoning on various reproductive parameters in mice, with a specific focus on the influence of time. A total of one hundred and forty-four male mice were randomly assigned to six groups. The control (C) group received only water and a standard diet without any interventions. The Cd group received a single intraperitoneal dose of cadmium chloride at 1.5 mg/kg. The cadmium intervention groups were administered broccoli extract at dosages of 100 mg/kg (Cd + B100), 200 mg/kg (Cd + B200), and 300 mg/kg (Cd + B300), respectively. Additionally, the Cd + VC group was treated with cadmium and vitamin C at 200 mg/kg intraperitoneally for a duration of 28 days. At the end of each week (four stages), five animals were randomly chosen from each group. Epididymal sperm were subjected to analysis for sperm parameters, while testicular tissue sections were examined for histological studies, apoptosis index, and markers of oxidative stress. The influence of time on body and testis weight gain was notably significant in the Cd + B300 and Cd + VC groups (p = 0.001). In all groups, except for Cd + B100, there were marked increases in spermatogenic cell lines and the Johnson coefficient compared to the Cd group (p = 0.001). These changes were particularly pronounced in the Cd + VC and Cd + B300 groups with respect to time (p < 0.001). Furthermore, there was a discernible positive impact of time on sperm count in the high-dose broccoli and vitamin C groups, although this effect did not reach significance in terms of sperm motility and vitality. Over time, the levels of superoxide dismutase (SOD) and catalase (CAT) enzymes increased, while malondialdehyde (MDA) levels decreased in the Cd + VC, Cd + B200, and Cd + B300 groups (p = 0.001). The apoptosis index in testicular tissue reached its highest level in the Cd group and its lowest level in the Cd + B300 and Cd + VC groups during the fourth week (p < 0.05). Linolenic acid, indole, and sulforaphane were identified as the most potent compounds in broccoli during this intervention. Consequently, vitamin C and broccoli extract at a dosage of 300 mg/kg demonstrated significant enhancements in reproductive performance in cases of cadmium poisoning. Overall, the influence of time significantly amplified the process of spermatogenesis and sperm production, with no observable changes in sperm viability and motility.

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期刊介绍： Biological Trace Element Research provides a much-needed central forum for the emergent, interdisciplinary field of research on the biological, environmental, and biomedical roles of trace elements. Rather than confine itself to biochemistry, the journal emphasizes the integrative aspects of trace metal research in all appropriate fields, publishing human and animal nutritional studies devoted to the fundamental chemistry and biochemistry at issue as well as to the elucidation of the relevant aspects of preventive medicine, epidemiology, clinical chemistry, agriculture, endocrinology, animal science, pharmacology, microbiology, toxicology, virology, marine biology, sensory physiology, developmental biology, and related fields.

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