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第十六届中国国际花卉园艺展览会.pdf

来源：花匠小妙招时间：2024-09-16 20:54

第十六届中国国际花卉园艺展览会

热带作物学报 2014，35(10)：1969—1974 ChineseJournalofTropicalCrops 番茄S1ARF2基因表达模式及其在果实发育中的功能分析杨枭，任振新，林冬波，先志强，李正国妻重庆享市高校功能鑫基篙因蔫与簧调控囊新技术重点实墓验室重王庆400030 摘要为了分析 SlARF2基因在番茄生长发育过程中的功能．利用定量 PCR技术分析 SlARF2基因的表达模式，发现SlARF2基因在番茄花芽中表达量最高，在幼果中次之，说明SlARF2基因可能在番茄花果发育中起着重要作用。为进一步研究 SlARF2的功能，构建 S1ARF2基因超表达、抑制表达载体，农杆菌介导转化番茄成功获得了相应转基因植株。通过与野生型番茄植株花、果不同时期的表型进行比对分析，发现 SlARF2基因的超表达对花的形态学特征无明显影响。但能够显著提高植株的结实率、降低果实大小和重量。说明 s|A／RF2基因参与调控番茄花、果发育过程。关键词番茄；SA／RF2；表达模式；果实发育中图分类号 $641．2 文献标识码 A TheExpression Pattern ofSIARF2 in Tomato and the FunctiOn Analysisin Tomato FruitDevelopment YANG Xiao，REN Zhenxin，LIN Dongbo，XIAN Zhiqiang，LIZhengguo GeneticEngineeringResearchCenter,SchoolofLfieSciences，ChongqingUniversity／KeyLabofFu~twndGene andNewRegulationTechnologiesof ChongqingMunicipalEducationCommission,Chongqing400030,China Abstract To investigate thefunction ofSbtRF2 in tomato development，weanalyzed the expression pattern of S 足 by Real—time PCR．We ofund thatS／ARF2 washighly expressed in tomato bud and immature fruit． suggestingthatSlARF2playsan importantroleintomatoflowerand fruitdevelopment．Furthermore，we constructed SlARF2 overexpression and suppress expression vectors．By agrobacterium infection，we finally obtained the SlARF2 overexpression and suppress transgenetic tomatoes．Compared the transgenetic tomatoes to wild type tomato，we found thatSlARF2 had no affection in hte morphology offlower，however SlARF2 overexpression strongly enhanced the rape rate oftomato and decreased hte size oftomato furit．1’I1e study demonstrates that SlAR1；2 regulates hte developmentoftomato flowerand furit，and providesbasic reference forfurtherstudy of ．s F2 function． Key words Tomato；SlARF2；Expression pattern；Furitde