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一株抗根结线虫放线菌的筛选与鉴定

来源：花匠小妙招时间：2025-01-05 14:10

摘要： 从海南省东寨港红树林底泥中分离获得具有抗线虫活性的放线菌菌株HA11097。将该菌株发酵液稀释20倍和40倍时，抗根结线虫校正死亡率分别为54.2%和46.7%。16S rRNA基因序列分析表明，菌株HA11097属于链霉菌属(Streptomyces)中的成员，与S. aculeolatus的相似性最高，为98.9%。二者在系统发育树上处于同一分支，亲缘关系最近，DNA-DNA杂交值为94.4%。该菌的形态特征、培养特征以及生理生化特征与S. aculeolatus也基本一致。综合以上结果，鉴定菌株HA11097为针孢链霉菌Streptomyces aculeolatus，首次报道了其抗线虫活性。

关键词: 红树林, 放线菌, 根结线虫, 鉴定

Abstract: Root-knot nematode antagonistic strain HA11097 was isolated from mud samples collected from Dongzhaigang mangroves in Hainan province. The mortality rates of the fermentation broth against root-knot nematode were 54.2% and 46.7% while diluted 20-fold and 40-fold, respectively. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain HA11097 should belong to a member of the genus Streptomyces, and closely related to S. aculeolatus, with the highest 16S rRNA gene similarity of 98.9%. In the phylogenetic tree, HA11097 was the closest relationship with S. aculeolatus, which both located on the same branch. Their DNA-DNA hybridization value was 94.4%. The morphological, cultural characteristics and the results of physiological and biochemical properties of the strain were in well agreement with S. aculeolatus. On the basis of these data, strain HA11097 was identified as Streptomyces aculeolatus. The article first reported that the strain of anti-nematode activity.

Key words: Mangrove, Actinomycetes, Root-knot nematodes, Identification