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独脚金离体培养与快速繁殖

来源：花匠小妙招时间：2025-01-05 10:36

摘要： 采用茎段作为外植体，建立独脚金Striga asiatica的组织培养快速繁殖方法。结果显示，0.1%升汞溶液处理7～9 min是较有效的消毒方法；诱导不定芽增殖培养基适宜配方为MS + 6-BA 3.0 mg·L-1 + IBA 0.1 mg·L-1；生根培养基配方为MS + NAA 0.1 mg·L-1 + IBA 0.5 mg·L-1，培育周期为3个月。

关键词: 独脚金, 组织培养, 快速繁殖

Abstract: Stems of Striga asiatica were used as the explants to establish optimum conditions for rapid propagation in vitro. The results showed that disinfecting the stems 7—9 min with 0.1% mercuric chloride solution was the best methods; MS medium with 3.0 mg·L-1 6-BA and 0.1 mg·L-1 IBA was optimum for inducing adventitious and proliferation reproduction. The suitable medium for rooting was MS + 0.1 mg·L-1 NAA + 0.5 mg·L-1 IBA.

Key words: Striga asiatica, tissue culture, rapid propagation

中图分类号:

Q943.1

引用本文

蔡时可,魏杰,李战超,王继华. 独脚金离体培养与快速繁殖[J]. 亚热带植物科学, 2017, 46(4): 375-378.

CAI Shi-ke,WEI Jie,LI Zhan-chao,WANG Ji-hua. In vitro Culture and Rapid Propagation of Striga asiatica[J]. Subtropical Plant Science, 2017, 46(4): 375-378.

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链接本文:http://www.yrdzwkx.com/CN/10.3969/j.issn.1009-7791.2017.04.015

http://www.yrdzwkx.com/CN/Y2017/V46/I4/375