《中药大辞典》记载的抗真菌中药体外药效再评价

摘要：

目的 对《中药大辞典》中记载有抗真菌药理作用的127种中药醇提物进行体外抗深部感染真菌（白念珠菌、新生隐球菌、烟曲霉）的研究。方法 采用75%乙醇超声提取法对127种中药进行统一提取，参考CLSI微量液基稀释法M27-A3和M38-A2方案进行抗白念珠菌、新生隐球菌、烟曲霉的最低抑菌浓度（MIC）测定，并进一步考察其抗菌谱及抑制菌丝作用。结果 在2 mg/mL浓度下，仅博落回等17种中药、月季花等34种中药、黄柏等5种中药分别对白念珠菌、新生隐球菌、烟曲霉有抑制作用，其中博落回和粉萆薢的抗菌谱广；在4 mg/mL浓度下，127种中药中仅粉萆薢和藜芦能完全抑制菌丝生长，最低浓度分别为200μg/mL和500μg/mL。结论 首次用统一标准对《中药大辞典》中记载的抗真菌中药进行体外活性筛选，并对其药效进行再评价，为新型抗真菌药物开发提供理论依据和参考。

关键词: 《中药大辞典》, 抗真菌, 中药, 白念珠菌, 新生隐球菌, 烟曲霉

Abstract:

Objective To investigate the antifungal activity of 127 types of the traditional Chinese medicines (TCMs) with the so-called antifungal activities recorded in ‘Zhong Yao Da Ci Dian’ against Candida albicans,Cryptococcus neoformans and Aspergillus fumigatus in vitro.Methods The portion from each TCM by 75% alcohol extracts were determined for antifungal activity against C. albicans, C. neoformans and A. fumigatus by using the standard CLSI M27-A3 and M38-A2 broth microdilution assays,and further to study the antifungal spectrum and inhibition of hyphae.Results The ethanol extracts from 17 TCMs exhibited the inhibitory activity against C. albicans with MICs ranged from 15.625 to 2 000 μg/mL.34 kinds of ethanol extracts from TCMs had the inhibitory effect against C. neoformans.5 ethanol extracts from TCMs had the inhibitory activity of inhibiting A. fumigatus.Conclusion The antifungal activities of TCMs which has been recorded in ‘Zhong Yao Da Ci Dian’ are measured in vitro by the consistent standard method for the first time,providing the experimental basis for investigation of new antifungal agents.

Key words: Zhong Yao Da Ci Dian', antifungal agents, minimal inhibitory concentration, Candida albicans, Cryptococcus neoformans, Aspergillus fumigatus

中图分类号: 

R978.5

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