霍山石斛与铁皮石斛的体外生物活性比较

MA Rong-feng, TANG Chuan, WANG Wen-han, YANG Yan, TIAN Xiao-jun, DAI Ya-feng, WANG Sheng-gui. Comparison of Biological Activities in Vitro of Dendrobium huoshanense and Dendrobium officinale[J]. Science and Technology of Food Industry, 2019, 40(12): 26-30,37. DOI: 10.13386/j.issn1002-0306.2019.12.005

Citation: MA Rong-feng, TANG Chuan, WANG Wen-han, YANG Yan, TIAN Xiao-jun, DAI Ya-feng, WANG Sheng-gui. Comparison of Biological Activities in Vitro of Dendrobium huoshanense and Dendrobium officinale[J]. Science and Technology of Food Industry, 2019, 40(12): 26-30,37. DOI: 10.13386/j.issn1002-0306.2019.12.005

霍山石斛与铁皮石斛的体外生物活性比较

马荣锋,  唐川1,  汪雯瀚2,  杨焱2,  田小军1,  戴亚峰1,  王升贵1, , 

1. 九仙尊霍山石斛股份有限公司, 上海 201199;

2. 农业部南方食用菌资源利用重点实验室, 国家食用菌工程技术研究中心, 上海市农业科学院食用菌研究所, 上海 201403

详细信息

作者简介:

马荣锋(1983-),女,硕士,研究方向:植物多糖及应用,E-mail:marongf@jgsteel.cn。

通讯作者:

王升贵( 1982-),男,本科,执业药师,研究方向:植物多糖及应用,E-mail:wangshenggui@jgsteel.cn。

中图分类号: TS255.1

计量 文章访问数:  0139 HTML全文浏览量:  06 PDF下载量:  09 出版历程 收稿日期:  2018-10-28 网络出版日期:  2020-11-12 刊出日期:  2019-06-14

Comparison of Biological Activities in Vitro of Dendrobium huoshanense and Dendrobium officinale

1. Jiuxianzun Dendrobium huoshanense Co., Ltd., Shanghai 201199, China;

2. Key Laboratory of Edible Fungi Resources and Utilization(South), Ministry of Agriculture, P. R. China, National Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China

摘要

摘要: 目的:比较霍山石斛与铁皮石斛(乐清、云南)3种石斛提取物对体外免疫、抗氧化、细胞氧化损伤、抗肿瘤和抑制α-葡萄糖苷酶活性的影响。方法:将3种石斛水提物配置成不同浓度溶液,分别与对照品一起进行体外测定刺激巨噬细胞RAW264.7释放NO的活性,清除ABTS自由基、FRAP法抗氧化能力和阻止H2O2诱导PC12细胞衰老能力,抑制肝癌细胞HepG2活性和胰岛素抵抗HepG2模型细胞内α-葡萄糖苷酶的活性研究。结果:霍山石斛提取物具有最优的体外刺激RAW264.7细胞释放NO的活性,更好的ABTS自由基清除能力和FRAP能力。200 μg·mL-1霍山石斛提取物对H2O2诱导的PC12细胞具有保护作用,修复率为40.52%。此外3种石斛提取物均具有一定的抑制HepG2肿瘤细胞增值的活性。与胰岛素抵抗模型组相比,在低浓度(100 μg·mL-1)时,3个样品均对细胞内α-葡萄糖苷酶的活性无抑制作用,在中高浓度时霍山石斛提取物(200和400 μg·mL-1)具有一定的抑制活性,表现出较好的降血糖作用。结论:霍山石斛相较于铁皮石斛在增强免疫、抗肿瘤、抗氧化、延缓衰老及降血糖等体外生物活性方面具有更好的作用。

Abstract: Objective:This study was designed to compare biological activities of Dendrobium. huoshanense and Dendrobium officinale(Yueqing,Yunnan)in vitro. The immunity activities,antioxidant activities,cell oxidative stress injury,antitumor activities and α-glucosidase inhibitoryassay were analyzed. Method:Three kinds of Dendrobium water extracts with different concentration were used to activity experiments in vitro,including the release of NO by stimulating macrophage RAW264.7,the ability of clearing ABTS free radicals,the total antioxidant capacity with FRAP method,the ability of preventing the cell PC12 aging induced by H2O2,the activity of hepatoma cell HepG2 and the activity of intracellular α-glucosidase of insulin resistance HepG2 model. Results:The results showed that the extract of Dendrobium huoshanense could significantly stimulate macrophages to release NO in vitro and strongest antioxidant of ABTS scavenging ability and Ferric ion reducing power. The Alamar Blue results indicated Dendrobium huoshanense extracts at the concentration of 200 μg/mL had better protective effect on activity decrease of PC12 cell induced by H2O2,and the recovery ratio was 40.52%. In addition,the water extracts from 3 species of Dendrobium could inhibit in growth of HepG2 cells. The Dendrobium huoshanense extracts(200,400 μg·mL-1)had stronger inhibitory effect on α-glucosidase than other fragments in insulin resistant model HepG2 cells,while water extracts from 3 species of Dendrobium had no activity at 100 μg·mL-1. Therefore,Dendrobium huoshanense had considerable anti-hyperglycemic activities. Conclusion:Dendrobium huoshanense had a better effect than Dendrobium officinale in enhancing biological activity such as immunity,anti-tumor,anti-oxidation,anti-aging and blood sugar lowering.

HTML全文

参考文献(0)

施引文献

资源附件(0)

计量 文章访问数:  HTML全文浏览量:  PDF下载量:  出版历程 收稿日期:  2018-10-28 网络出版日期:  2020-11-12 刊出日期:  2019-06-14

目录

相关知识

霍山铁皮石斛花的功效与作用
霍山铁皮石斛种植技术介绍
霍山铁皮石斛花，不止泡茶那么简单！功效更神奇...……
铁皮石斛浑身是宝，它的花也功效多多！
石斛药理作用研究进展
铁皮石斛的功效与作用 铁皮石斛怎么吃
九大仙草排名,霍山铁皮石斛多少钱一斤
中华九大仙草之首，霍山石斛不为人知的前世今生！
石斛——中华仙草
铁皮石斛花化学成分及抗氧化活性研究

网址: 霍山石斛与铁皮石斛的体外生物活性比较 https://www.huajiangbk.com/newsview1398102.html

上一篇: 霍山石斛用开水怎么泡,一次放几粒

下一篇: 霍山石斛的形态有什么特点？