首页分享温度和振动联合胁迫对花鲈有水保活运输中组织损伤及生化指标的影响

温度和振动联合胁迫对花鲈有水保活运输中组织损伤及生化指标的影响

来源：花匠小妙招时间：2024-12-29 13:33

图 1 不同温度和振动强度对花鲈CAT (a)、SOD (b)、GST (c)活性以及GLU (d)含量氧化应激的影响

不同的小写字母表示同一时间不同运输组的平均值有显著差异 (P<0.05)，下同。

Figure 1. Effects of different temperature and vibration intensity on CAT (a), SOD (b), GST (c) activity and GLU (d) content of L. japonicus

Different lowercase letters indicate that the average value of different transport groups at the same time has significant difference (P<0.05), the same below.

图 2 不同温度和振动强度对花鲈MDA含量(a)、ALT (b)和AST (c)活性的影响

Figure 2. Effects of different temperature and shock intensity on MDA content (a), ALT (b) and AST (c) activity of L. japonicus

花鲈鳃丝的扫描电子显微镜(SEM)显微照片

1. 0 h的鳃组织形态变化，2~7. A、B、C、D、E、F实验组运输12 h后鳃组织形态变化，8~13. A、B、C、D、E、F实验组运输24 h后鳃组织形态变化，白圈代表体表黏液，箭头代表鳃丝紊乱。

Scanning electron microscope (SEM) micrograph of L. japonicus gill-filaments

1. the morphological changes of gill tissue at 0 h, 2-7. the morphological changes of gill tissue after 12 h transportation for experimental groups A, B, C, D, E, and F, 8-13. the morphological changes of gill tissue after 24 h transportation for experimental groups A, B, C, D, E, and F; the white circle represents the body surface mucus, and the arrow represents the gill-filaments disorder.

图 3 不同温度和振动强度对花鲈ACP (a)、AKP (b)、IgM (c)和LZM (d)活性的影响

Figure 3. Effects of different temperature and vibration intensity on ACP (a), AKP (b), IgM (c) and LZM (d) activities of L. japonicus

图 4 不同温度和振动强度对运输水质的影响

Figure 4. Influence of different temperature andvibration intensity on transportation water quality

表 1 不同温度及振动强度实验组

Table 1 Different temperature and vibration strength test groups

实验组标号
test group label处理方式
treatment A20 °C运输温度+0 r/min振荡强度B20 °C运输温度+20 r/min振荡强度C20 °C运输温度+50 r/min振荡强度D12 °C运输温度+0 r/min振荡强度E12 °C运输温度+20 r/min振荡强度F12 °C运输温度+50 r/min振荡强度

表 2 不同运输组花鲈的存活率

Table 2 Survival rate of L. japonicus in different transportation groups and preservation time %

运输组
transport group 保活时间/h　time 0 12 24 36 48 A 100.00 100.00 72.70 0.00 0.00 B 100.00 100.00 62.50 0.00 0.00 C 100.00 100.00 18.20 0.00 0.00 D 100.00 100.00 100.00 71.40 50.00 E 100.00 100.00 100.00 75.00 0.00 F 100.00 100.00 100.00 42.90 0.00 [1] 李秀霞, 刘孝芳, 刘宏影, 等. 超声波辅助冷冻与低温速冻对海鲈鱼冰晶形态及冻藏期间鱼肉肌原纤维蛋白结构的影响[J]. 中国食品学报, 2021, 21(10): 169-176.

Li X X, Liu X F, Liu H Y, et al. Effects of ultrasound-assisted freezing and cryogenic quick freezing on ice crystal morphology and myofibrin structure of Sea bass (Lateolabrax japonicus) during frozen storage[J]. Journal of Chinese Institute of Food Science and Technology, 2021, 21(10): 169-176.

[2]

Purbosari N, Warsiki E, Syamsu K, et al. Natural versus synthetic anesthetic for transport of live fish: a review[J]. Aquaculture and Fisheries, 2019, 4(4): 129-133. doi: 10.1016/j.aaf.2019.03.002

[3]

Wang Q, Mei J, Cao J, et al. Effects of Melissa officinalis L. Essential oil in comparison with anaesthetics on gill tissue damage, liver metabolism and immune parameters in Sea bass (Lateolabrax maculatus) during Simulated Live Transport[J]. Biology, 2021, 11(1): 11. doi: 10.3390/biology11010011

[4] 张宇雷, 管崇武. 船载振动胁迫对斑石鲷影响实验研究[J]. 渔业现代化, 2017, 44(3): 29-34. doi: 10.3969/j.issn.1007-9580.2017.03.005

Zhang Y L, Guan C W. Experimental study on effects of ship vibration stress on Oplegnathus punctatus[J]. Fishery Modernization, 2017, 44(3): 29-34 (in Chinese). doi: 10.3969/j.issn.1007-9580.2017.03.005

[5] 张饮江, 黎臻, 谢文博, 等. 金鱼对低温、振动胁迫应激反应的试验研究[J]. 水产科技情报, 2012, 39(3): 116-122. doi: 10.3969/j.issn.1001-1994.2012.03.003

Zhang Y J, Li Z, Xie W B, et al. Experimental study on stress response of goldfish to low temperature and vibration stress[J]. Fisheries Science & Technology Information, 2012, 39(3): 116-122 (in Chinese). doi: 10.3969/j.issn.1001-1994.2012.03.003

[6]

Topal A, Özdemir S, Arslan H, et al. How does elevated water temperature affect fish brain? (A neurophysiological and experimental study: assessment of brain derived neurotrophic factor, cFOS, apoptotic genes, heat shock genes, ER-stress genes and oxidative stress genes)[J]. Fish & Shellfish Immunology, 2021, 115: 198-204.

[7] 张克烽, 王艺磊, 张子平. 鲍高温胁迫响应机制的研究进展[J]. 水产学报, 2023, 47(5): 059601.

Zhang K F, Wang Y L, Zhang Z P. Research progress on response mechanism to high temperature stress in abalone[J]. Journal of Fisheries of China, 2023, 47(5): 059601 (in Chinese).

[8]

Chang C H, Zhou X W, Wang Y C, et al. Differential effects of hypothermal stress on lactate metabolism in fresh water- and seawater-acclimated milkfish, Chanos chanos[J]. Comparative Biochemistry and Physiology-Part A: Molecular & Integrative Physiology, 2020, 248: 110744.

[9] 王琪, 梅俊, 谢晶. 低温保活运输对海鲈鱼应激及品质的影响[J]. 中国食品学报, 2022, 22(7): 203-213, doi: 10.16429/j.1009-7848.2022.07.021

Wang Q, Mei J, Xie J. Effects of low temperature and alive transportation on stress and meat quality of sea bass (Lateolabrax maculatus)[J]. Journal of Chinese Institute of Food Science and Technology, 2022, 22(7): 203-213, doi: 10.16429/j.1009-7848.2022.07.021

[10] 朱学旺, 田光明. 制定室内模拟运输试验条件的一种方法[J]. 环境技术, 2009, 27(3): 7-9. doi: 10.3969/j.issn.1004-7204.2009.03.005

Zhu X W, Tian G M. A study on the lab test conditions for transportation environment simulation[J]. Environmental Technology, 2009, 27(3): 7-9 (in Chinese). doi: 10.3969/j.issn.1004-7204.2009.03.005

[11] 张玉晗, 谢晶. 低温休眠预处理对花鲈无水保活效果的影响[J]. 食品科学, 2018, 39(23): 221-226. doi: 10.7506/spkx1002-6630-201823033

Zhang Y H, Xie J. Effect of precooling treatment on survival of Lateolabrax maculatus during live transportation without using water[J]. Food Science, 2018, 39(23): 221-226 (in Chinese). doi: 10.7506/spkx1002-6630-201823033

[12]

Madeira D, Narciso L, Cabral H N, et al. Influence of temperature in thermal and oxidative stress responses in estuarine fish[J]. Comparative Biochemistry and Physiology-Part A: Molecular & Integrative Physiology, 2013, 166(2): 237-243.

[13]

Vinagre C, Madeira D, Narciso L, et al. Effect of temperature on oxidative stress in fish: lipid peroxidation and catalase activity in the muscle of juvenile seabass, Dicentrarchus labrax[J]. Ecological Indicators, 2012, 23: 274-279. doi: 10.1016/j.ecolind.2012.04.009

[14]

Otitoloju A, Olagoke O. Lipid peroxidation and antioxidant defense enzymes in Clarias gariepinus as useful biomarkers for monitoring exposure to polycyclic aromatic hydrocarbons[J]. Environmental Monitoring and Assessment, 2011, 182(1): 205-213.

[15]

Yang S, Yan T, Wu H, et al. Acute hypoxic stress: effect on blood parameters, antioxidant enzymes, and expression of HIF-1alpha and GLUT-1 genes in largemouth bass (Micropterus salmoides)[J]. Fish & Shellfish Immunology, 2017, 67: 449-458.

[16]

Cheng C H, Guo Z X, Luo S W, et al. Effects of high temperature on biochemical parameters, oxidative stress, DNA damage and apoptosis of pufferfish (Takifugu obscurus)[J]. Ecotoxicology and Environmental Safety, 2018, 150: 190-198. doi: 10.1016/j.ecoenv.2017.12.045

[17]

Kregel K C. Invited review: heat shock proteins: Modifying factors in physiological stress responses and acquired thermotolerance[J]. Journal of Applied Physiology, 2002, 92(5): 2177-2186. doi: 10.1152/japplphysiol.01267.2001

[18]

Klein R D, Borges V D, Rosa C E, et al. Effects of increasing temperature on antioxidant defense system and oxidative stress parameters in the Antarctic fish Notothenia coriiceps and Notothenia rossii[J]. Journal of Thermal Biology, 2017, 68: 110-118. doi: 10.1016/j.jtherbio.2017.02.016

[19]

Parihar M S, Dubey A K, Javeri T, et al. Changes in lipid peroxidation, superoxide dismutase activity, ascorbic acid and phospholipid content in liver of freshwater catfish Heteropneustes fossilis exposed to elevated temperature[J]. Journal of Thermal Biology, 1996, 21(5-6): 323-330. doi: 10.1016/S0306-4565(96)00016-2

[20]

Luo S W, Cai L, Liu Y, et al. Functional analysis of a dietary recombinant fatty acid binding protein 10 (FABP10) on the Epinephelus coioides in response to acute low temperature challenge[J]. Fish & Shellfish Immunology, 2014, 36(2): 475-484.

[21]

Chang C H, Mayer M, Rivera-Ingraham G, et al. Effects of temperature and salinity on antioxidant responses in livers of temperate (Dicentrarchus labrax) and tropical (Chanos chanos) marine euryhaline fish[J]. Journal of Thermal Biology, 2021, 99: 103016. doi: 10.1016/j.jtherbio.2021.103016

[22]

Cheng C H, Ye C X, Guo Z X, et al. Immune and physiological responses of pufferfish (Takifugu obscurus) under cold stress[J]. Fish & Shellfish Immunology, 2017, 64: 137-145.

[23] 李庆昌, 陈小明, 刘贤德. 突变高温胁迫对大黄鱼血清生理指标的影响[J]. 渔业研究, 2016, 38(6): 437-444.

Li Q C, Chen X M, Liu X D. Acute heat stress on the influence of large yellow croaker (Larimichthys crocea) serum physiological indicators[J]. Journal of Fisheries Research, 2016, 38(6): 437-444 (in Chinese).

[24]

Lee P P, Lin Y H, Chen M C, et al. Dietary administration of sodium alginate ameliorated stress and promoted immune resistance of grouper Epinephelus coioides under cold stress[J]. Fish & Shellfish Immunology, 2017, 65: 127-135.

[25] 曹杰, 王琪, 梅俊, 等. 有水与无水保活运输对大菱鲆生理应激及鱼肉品质的影响[J]. 水产学报, 2021, 45(7): 1034-1042.

Cao J, Wang Q, Mei J, et al. Effects of transport in water and waterless transport on physiological stress and flesh quality of turbot (Scophthalmus maximus)[J]. Journal of Fisheries of China, 2021, 45(7): 1034-1042 (in Chinese).

[26] 张坤, 周结, 范秀萍, 等. 海水温度对金鲳鱼保活过程中应激和代谢的影响[J]. 包装工程, 2022, 43(9): 92-99.

Zhang K, Zhou J, Fan X P, et al. Effect of temperature on stress and metabolism of golden pompano (Trachinotus ovatus) during keep-alive[J]. Packaging Engineering, 2022, 43(9): 92-99 (in Chinese).

[27]

Sandodden R, Finstad B, Iversen M. Transport stress in Atlantic salmon (Salmo salar L.): anaesthesia and recovery[J]. Aquaculture Research, 2001, 32(2): 87-90. doi: 10.1046/j.1365-2109.2001.00533.x

[28]

Barcellos L J G, Nicolaiewsky S, De Souza S M G, et al. Plasmatic levels of cortisol in the response to acute stress in Nile tilapia, Oreochromis niloticus (L.), previously exposed to chronic stress[J]. Aquaculture Research, 1999, 30(6): 437-444. doi: 10.1046/j.1365-2109.1999.00348.x

[29] 杜浩, 危起伟, 甘芳, 等. 美洲鲥应激后皮质醇激素和血液生化指标的变化[J]. 动物学杂志, 2006, 41(3): 80-84. doi: 10.3969/j.issn.0250-3263.2006.03.015

Du H, Wei Q W, Gan F, et al. Changes in serum cortisol and blood biochemical parameters after stress in American shad[J]. Chinese Journal of Zoology, 2006, 41(3): 80-84 (in Chinese). doi: 10.3969/j.issn.0250-3263.2006.03.015

[30] 贾明亮. 低温胁迫对奥尼罗非鱼的生长、肌肉组成和血液生理生化指标的影响[D]. 湛江: 广东海洋大学, 2010.

Jia M L. The Effect of low temperature stress on growth、muscle composition and hematological and biochemical indices of tilapia (Oreochromis niloticus × O. aures)[D]. Zhanjiang: Guangdong Ocean University, 2010 (in Chinese).

[31]

Gao Q, Liu B, Shan F, et al. Effects of oxidized fish oil on digestive enzyme activity and antioxidant system in Macrobrachium rosenbergii post-larvae[J]. Aquaculture Reports, 2022, 23: 101062. doi: 10.1016/j.aqrep.2022.101062

[32]

Zhang H Z, Fang D, Mei J, et al. A preliminary study on the effects of nitrite exposure on hematological parameters, oxidative stress, and immune-related responses in Pearl gentian grouper[J]. Fishes, 2022, 7(5): 235. doi: 10.3390/fishes7050235

[33]

Wang Y F, Li C J, Pan C L, et al. Alterations to transcriptomic profile, histopathology, and oxidative stress in liver of pikeperch (Sander lucioperca) under heat stress[J]. Fish & Shellfish Immunology, 2019, 95: 659-669.

[34]

Malandrakis E E, Dadali O, Golomazou E, et al. DNA damage and differential gene expression associated with physical stress in gilthead seabream (Sparus aurata)[J]. General and Comparative Endocrinology, 2016, 236: 98-104. doi: 10.1016/j.ygcen.2016.07.009

[35] 胡毅, 黄云, 钟蕾, 等. 氨氮胁迫对青鱼幼鱼鳃丝Na+/K+-ATP酶、组织结构及血清部分生理生化指标的影响[J]. 水产学报, 2012, 36(4): 538-545. doi: 10.3724/SP.J.1231.2012.27713

Hu Y, Huang Y, Zhong L, et al. Effects of ammonia stress on the gill Na+/K+-ATPase, microstructure and some serum physiological-biochemical indices of juvenile black carp (Mylopharyngodon piceus)[J]. Journal of Fisheries of China, 2012, 36(4): 538-545 (in Chinese). doi: 10.3724/SP.J.1231.2012.27713

[36] 冯广朋, 庄平, 章龙珍, 等. 温度对中华鲟幼鱼代谢酶和抗氧化酶活性的影响[J]. 水生生物学报, 2012, 36(1): 137-142.

Feng G P, Zhuang P, Zhang L Z, et al. Effects of water temperature on metabolic enzyme and antioxidase activities in juvenile Chinese sturgeon (Acipenser sinensis)[J]. Acta Hydrobiologica Sinica, 2012, 36(1): 137-142 (in Chinese).

[37]

Li C J, Wang Y F, Wang G C, et al. Physicochemical changes in liver and Hsc70 expression in pikeperch Sander lucioperca under heat stress[J]. Ecotoxicology and Environmental Safety, 2019, 181: 130-137. doi: 10.1016/j.ecoenv.2019.05.083

[38]

Ming J H, Xie J, Xu P, et al. Effects of emodin and vitamin C on growth performance, biochemical parameters and two HSP70s mRNA expression of Wuchang bream (Megalobrama amblycephala Yih) under high temperature stress[J]. Fish & Shellfish Immunology, 2012, 32(5): 651-661.

[39]

Xu Z K, Zhang H Z, Guo M J, et al. Analysis of acute nitrite exposure on physiological stress response, oxidative stress, gill tissue morphology and immune response of Large yellow croaker (Larimichthys crocea)[J]. Animals, 2022, 12(14): 1791. doi: 10.3390/ani12141791

[40]

Al-Zaidan A S, Endo M, Maita M, et al. A toxicity bioassay study concerning the effect of un-ionized ammonia on the mucus cells response originating from the gills of zebrafish Danio rerio[J]. Fisheries Science, 2013, 79(1): 129-142. doi: 10.1007/s12562-012-0573-6

[41]

Naiel M A E, El-hameed S A A A, Arisha A H, et al. Gum Arabic-enriched diet modulates growth, antioxidant defenses, innate immune response, intestinal microbiota and immune related genes expression in tilapia fish[J]. Aquaculture, 2022, 556: 738249. doi: 10.1016/j.aquaculture.2022.738249

[42]

Ndong D, Chen Y Y, Lin Y H, et al. The immune response of tilapia Oreochromis mossambicus and its susceptibility to Streptococcus iniae under stress in low and high temperatures[J]. Fish & Shellfish Immunology, 2007, 22(6): 686-694.

[43]

Fevolden S E, Røed K H, Fjalestad K. A combined salt and confinement stress enhances mortality in rainbow trout (Oncorhynchus mykiss) selected for high stress responsiveness[J]. Aquaculture, 2003, 216(1-4): 67-76. doi: 10.1016/S0044-8486(02)00131-X

[44] 李伟业, 徐志进, 殷小龙, 等. 温度对美洲黑石斑(Centropristis striata)幼鱼生长和免疫因子活力与相关基因表达的影响[J]. 海洋与湖沼, 2021, 52(3): 708-717. doi: 10.11693/hyhz20200900257

Li W Y, Xu Z J, Yin X L, et al. Effects of temperature on growth, immune factor activity and related gene expression of juvenile Centropristis striata[J]. Oceanologia et Limnologia Sinica, 2021, 52(3): 708-717 (in Chinese). doi: 10.11693/hyhz20200900257

[45] 米博瀚, 赵雪飞, 高珊, 等. 碱胁迫对瓦氏雅罗鱼AKP基因表达及生理应答的影响[J]. 农业生物技术学报, 2020, 28(6): 1052-1059.

Mi B H, Zhao X F, Gao S, et al. Effects of alkaline stress on AKP gene expression and physiology response in Leuciscus waleckii[J]. Journal of Agricultural Biotechnology, 2020, 28(6): 1052-1059 (in Chinese).

[46] 张龙岗, 安丽, 孙栋, 等. 盐度对澳洲宝石鲈幼鱼3个免疫因子活力的影响[J]. 水生态学杂志, 2011, 32(6): 110-114.

Zhang L G, An L, Sun D, et al. Effect of salinity on activity of three immune factors of juvenile Scortum barcoo[J]. Journal of Hydroecology, 2011, 32(6): 110-114 (in Chinese).

[47] 张小明, 土志涵, 肖海明, 等. 低氧胁迫对瘤背石磺能量代谢、氧化应激和免疫功能的影响[J/OL]. 水产学报, 1-11 [2022-01-11]. http://kns.cnki.net/kcms/detail/31.1283.S.20220111.0950.002.html.

Zhang X M, Tu Z H, Xiao H M, et al. Effects of hypoxia stress on energy metabolism, oxidative stress and immune function[J/OL]. Journal of Fisheries of China, 1-11 [2022-01-11]. http://kns.cnki.net/kcms/detail/31.1283.S.20220111.0950.002.html (in Chinese).

[48] 白海文. 温度对施氏鲟幼鱼生长、消化及免疫功能影响的研究[D]. 上海: 上海海洋大学, 2012.

Bai H W. A studies on the effects of water temperature on growth, digestion and immune function of juvenile Acipenser schrenckii[D]. Shanghai: Shanghai Ocean University, 2012 (in Chinese).

[49] 李文龙, 梁兴明, 梁萌青, 等. 温度对大菱鲆幼鱼生长及免疫相关酶活性的影响[J]. 水产科学, 2017, 36(3): 311-316.

Li W L, Liang X M, Liang M Q, et al. Effects of temperature on growth and enzyme activity related to immunity in juvenile turbot Scophthalmus maximus[J]. Fisheries Science, 2017, 36(3): 311-316 (in Chinese).

[50]

Bilal S, Lie K K, Karlsen O A, et al. Characterization of IgM in Norwegian cleaner fish (lumpfish and wrasses)[J]. Fish & Shellfish Immunology, 2016, 59: 9-17.

[51] 何杰, 强俊, 徐跑, 等. 低温驯化下4种不同品系罗非鱼血清皮质醇与免疫相关指标的变化[J]. 中国水产科学, 2014, 21(2): 266-274.

He J, Qiang J, Xu P, et al. Change in serum cortisol and immune-related indices in four strains of tilapia during low-temperature acclimation[J]. Journal of Fishery Sciences of China, 2014, 21(2): 266-274 (in Chinese).

[52] 范秀萍. 珍珠龙胆石斑鱼低温休眠无水保活的胁迫响应与机制研究[D]. 湛江: 广东海洋大学, 2019.

Fan X P. Stress response and mechanism of waterless preservation after low-temperature induced dormancy on pearl gentian grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus)[D]. Zhanjiang: Guangdong Ocean University, 2019 (in Chinese).

[53]

Zhang Y J, Xiao X Q, Yan L, et al. Survival prediction system for waterless live Chinese sturgeon transportation based on temperature related glucose changes[J]. Journal of Food Process Engineering, 2018, 41(2): e12646. doi: 10.1111/jfpe.12646

[54]

Stockman J, Weber E S P, Kass P H, et al. Physiologic and biochemical measurements and response to noxious stimulation at various concentrations of MS-222 in Koi (Cyprinus carpio)[J]. Veterinary Anaesthesia and Analgesia, 2013, 40(1): 35-47. doi: 10.1111/j.1467-2995.2012.00739.x