转基因玉米ND207转化事件特异性定性PCR检测方法及其标准化
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时间:2024-12-23 13:03
[1] 吕山花, 常汝镇, 陶波, 李向华, 栾凤侠, 郭珊花, 邱丽娟. 抗草甘膦转基因大豆PCR检测方法的建立与应用. 中国农业科学, 2003, 36: 883-887. Lyu S H, Chang R Z, Tao B, Li X H, Luan F X, Guo S H, Qiu L J. Methodological research on PCR based detection of genetically modified soybean resistant to glyphosate. Sci Agric Sin, 2003, 36: 883-887. (in Chinese with English abstract)[2] 查中萍, 万丙良, 殷得所, 杜雪树, 李进波, 夏明元, 戚华雄, 李园梦. 转Cry1Ab/Cry1Ac基因水稻TT51-1中外源基因多重PCR检测方法. 湖北农业科学, 2019, 58: 232-235. Zha Z P, Wan B L, Yin D S, Du X S, Li J B, Xia M Y, Qi H X, Li Y M. A tri-primer multiplex PCR method for the detection of exogenous genes in the Cry1Ab/Cry1Ac transgenic rice TT51-1. Hubei Agric Sci, 2019, 58: 232-235 (in Chinese with English abstract).[3] 常丽娟, 宋君, 雷绍荣, 尹全, 王东, 刘文娟, 张富丽. 转基因玉米MIR604结构特异片段实时荧光定量检测方法的建立. 江苏农业学报, 2015, 31: 971-974. Chang L J, Song J, Lei S R, Yin Q, Wang D, Liu W J, Zhang F L. A construct-specific real-time PCR for quantitative detection of genetically modified maize MIR604. Jiangsu J Agric Sci, 2015, 31: 971-974. (in Chinese with English abstract)[4] 李葱葱, 谢苹, 董立明, 夏蔚, 兰青阔, 闫伟, 龙丽坤, 李飞武. 抗虫耐除草剂玉米 GH5112E-117C定性PCR检测方法. 生物技术通报, 2020, 36(5): 64-67.
doi: 10.13560/j.cnki.biotech.bull.1985.2019-0701 Li C C, Xie P, Dong L M, Xia W, Lan Q K, Yan W, Long L K, Li F W. Qualitative PCR assay for the detection of GH5112E-117C transgenic maize resistant to insects and herbicides. Biotechnol Bull, 2020, 36(5): 64-67. (in Chinese with English abstract)[5] Wu G, Wu Y H, Nie S J, Zhang L, Xiao L, Cao Y L, Lu C M. Real-time PCR method for detection of the transgenic rice event TT51-1. Food Chem, 119: 417-422.
doi: 10.1016/j.foodchem.2009.08.031[6] Zhang F L, Song J, Niu B, Yin Q, Chang L J, Wang D. An event-specific qualitative and real-time PCR detection of 98140 maize in mixed samples. Food Control, 2015, 57: 1-8.
doi: 10.1016/j.foodcont.2015.04.002[7] 翟勇, 武玉花, 吴刚, 曹应龙, 卢长明. 转基因玉米MON88017转化事件特异性定性PCR检测方法及其标准化. 农业生物技术学报, 2010, 6: 1208-1214. Zhai Y, Wu Y H, Wu G, Cao Y L, Lu C M. Event-specific PCR detection method of transgenic maize line MON88017 and its standardization. J Agric Biotechnol, 2010, 6: 1208-1214. (in Chinese with English abstract)[8] 农业部1193号公告-3-2009. 转基因植物及其产品成分检测抗虫水稻TT51-1及其衍生品种定性PCR方法. 北京: 中国标准出版社, 2009. Department of Agriculture Notice 1193-3-2009. Detection of Genetically Modified Plants and Derived Products-qualitative PCR Method for Insect Resistant Rice TT51-1 and Its Derivates. Beijing: Standards Press of China, 2009. (in Chinese)[9] 农业农村部公告第111号-8-2018. 转基因植物及其产品成分检测耐除草剂玉米COO10.1.1及其衍生品种定性PCR方法. 北京: 中国标准出版社, 2018. Ministry of Agriculture and Rural Affairs Announcement No.111-8-2018. Detection of Genetically Modified Plants and Derived Products-qualitative PCR Method for Herbicide-tolerant Maize C0010.1.1 and Its Derivates. Beijing: Standards Press of China, 2018. (in Chinese)[10] 农业农村部公告第323号-4-2020. 转基因植物及其产品成分检测耐除草剂棉花MON88701及其衍生品种定性PCR方法. 北京: 中国标准出版社, 2020. Ministry of Agriculture and Rural Affairs Announcement No.323-4-2020. Detection of Genetically Modified Plants and Derived Products-qualitative PCR Method for Herbicide-tolerant Cotton MON88701 and Its Derivates. Beijing: Standards Press of China, 2020. (in Chinese)[11] 农业农村部公告第323号-5-2020. 转基因植物及其产品成分检测抗虫大豆MON87751及其衍生品种定性PCR方法. 北京: 中国标准出版社, 2020. Ministry of Agriculture and Rural Affairs Announcement No.323-5-2020. Detection of Genetically Modified Plants and Derived Products-qualitative PCR Method for Insect Resistant Soybean MON87751 and Its Derivates. Beijing: Standards Press of China, 2020. (in Chinese)[12] 农业部2259号公告-4-2015. 转基因植物及其产品成分检测定性PCR方法制定指南. 北京: 中国标准出版社, 2015. Department of Agriculture Notice 2259-4-2015. Detection of Genetically Modified Plants and Derived Products—Guidelines for Establishing qualitative PCR Method. Beijing: Standards Press of China, 2015. (in Chinese)[13] ENGL. Definition of Minimum Performance Requirements for Analytical Methods of GMO Testing. European Network of GMO Laboratories, 2015.[14] SN/T 4561-2016. 转基因检测非标方法确认评价指南. 北京: 中国标准出版社, 2016. SN/T 4561-2016. Guideline for Validation of Genetically-Modified Organism Test Methods. Beijing: Standards Press of China, 2016. (in Chinese)[15] 袁磊, 孙红炜, 杨崇良. 转基因玉米MON88017旁侧序列分析及定性PCR检测. 作物学报, 2010, 36: 361-364.
doi: 10.3724/SP.J.1006.2010.00361 Yuan L, Sun H W, Yang C L. Analysis of junction sequence the transgenic maize MON88017 and the methods of qualitative PCR detection. Acta Agron Sin, 2010, 36: 361-364. (in Chinese with English abstract)
doi: 10.3724/SP.J.1006.2010.00361[16] 申爱娟, 陈松, 周晓婴, 戚存扣. 转基因油菜W-4 T-DNA旁侧序列分析与事件特异性检测. 江苏农业学报, 2014, 30(1): 10-20. Shen A J, Chen S, Zhou X Y, Qi C K. Analysis of the flanking sequence and event-specific detection of transgenic line W-4 of Brassica napus. J Agric Sci, 2014, 30(1): 10-20. (in Chinese with English abstract)[17] 张艳敏, 张红梅, 相金英, 郭秀林, 刘子会, 李国良, 陈受宜. 转BADH基因苜蓿T-DNA侧翼序列分析及转化事件特异性分析. 作物学报, 2011, 37: 397-404.
doi: 10.3724/SP.J.1006.2011.00397 Zhang Y M, Zhang H M, Xiang J Y, Guo X L, Liu Z H, Li G L, Chen S Y. Analysis of T-DNA flanking sequences and event specific detection of transgenic alfalfa with gene BADH. Acta Agron Sin, 2011, 37: 397-404. (in Chinese with English abstract)[18] 冯翠莲, 万玥, 冯小艳, 王俊刚, 赵婷婷, 王文治, 沈林波, 张树珍. 转基因甘蔗BtG-2的T-DNA侧翼序列分析及其转化事件特异性检测. 热带作物学报, 2021, 42: 2468-2477. Feng C L, Wan Y, Feng X Y, Wang J G, Zhao T T, Wang W Z, Shen L B, Zhang S Z. Analysis of the T-DNA Flanking sequence and event-specific detection for insect-resistant transgenic sugarcane BtG-2. Chin J Trop Crops, 2021, 42: 2468-2477. (in Chinese with English abstract)[19] Marmiroli N, Maestri E, Gulli M. Methods for detection of GMOs in food and feed. Anal Bioanal Chem, 2008, 392: 369-384.
doi: 10.1007/s00216-008-2303-6pmid: 18726090[20] 农业部2122号公告-8-2014. 转基因植物及其产品成分检测抗虫水稻TT51-1及其衍生品种定量PCR方法. 北京: 中国标准出版社, 2014. Department of Agriculture Notice 2122-8-2014. Detection of Genetically Modified Plants and Derived Products-quantitative PCR Method for Insect Resistant Rice TT51-1 and Its Derivates. Beijing: Standards Press of China, 2014. (in Chinese)[21] 农业农村部公告第323号-10-2020. 转基因植物及其产品成分检测耐除草剂大豆GTS40-3-2及其衍生品种定量PCR方法. 北京: 中国标准出版社, 2020. Ministry of Agriculture and Rural Affairs Announcement No.323-5-2020. Detection of Genetically Modified Plants and Derived Products-quantitative PCR Method for Herbicide-tolerant Soybean GTS40-3-2 and Its Derivates. Beijing: Standards Press of China, 2020. (in Chinese)[22] GB/T 38132-2019. 转基因植物品系定量检测数字PCR法. 北京: 中国标准出版社, 2019. GB/T 38132-2019. Quantitative Determination of Genetically Modified Plants by Digital PCR Method. Beijing: Standards Press of China, 2019. (in Chinese)[23] SN/T 5334.2-2020. 转基因植物产品的数字PCR检测方法第二部分:转基因大豆. 北京: 中国标准出版社, 2020. SN/T 5334.2-2020. Protocol of Digital PCR for Quantitatively Detecting Genetically Modified Plants and Their Derived Products-Part 2: Genetically Modified Soybean. Beijing: Standards Press of China, 2020. (in Chinese)
doi: 10.13560/j.cnki.biotech.bull.1985.2019-0701 Li C C, Xie P, Dong L M, Xia W, Lan Q K, Yan W, Long L K, Li F W. Qualitative PCR assay for the detection of GH5112E-117C transgenic maize resistant to insects and herbicides. Biotechnol Bull, 2020, 36(5): 64-67. (in Chinese with English abstract)[5] Wu G, Wu Y H, Nie S J, Zhang L, Xiao L, Cao Y L, Lu C M. Real-time PCR method for detection of the transgenic rice event TT51-1. Food Chem, 119: 417-422.
doi: 10.1016/j.foodchem.2009.08.031[6] Zhang F L, Song J, Niu B, Yin Q, Chang L J, Wang D. An event-specific qualitative and real-time PCR detection of 98140 maize in mixed samples. Food Control, 2015, 57: 1-8.
doi: 10.1016/j.foodcont.2015.04.002[7] 翟勇, 武玉花, 吴刚, 曹应龙, 卢长明. 转基因玉米MON88017转化事件特异性定性PCR检测方法及其标准化. 农业生物技术学报, 2010, 6: 1208-1214. Zhai Y, Wu Y H, Wu G, Cao Y L, Lu C M. Event-specific PCR detection method of transgenic maize line MON88017 and its standardization. J Agric Biotechnol, 2010, 6: 1208-1214. (in Chinese with English abstract)[8] 农业部1193号公告-3-2009. 转基因植物及其产品成分检测抗虫水稻TT51-1及其衍生品种定性PCR方法. 北京: 中国标准出版社, 2009. Department of Agriculture Notice 1193-3-2009. Detection of Genetically Modified Plants and Derived Products-qualitative PCR Method for Insect Resistant Rice TT51-1 and Its Derivates. Beijing: Standards Press of China, 2009. (in Chinese)[9] 农业农村部公告第111号-8-2018. 转基因植物及其产品成分检测耐除草剂玉米COO10.1.1及其衍生品种定性PCR方法. 北京: 中国标准出版社, 2018. Ministry of Agriculture and Rural Affairs Announcement No.111-8-2018. Detection of Genetically Modified Plants and Derived Products-qualitative PCR Method for Herbicide-tolerant Maize C0010.1.1 and Its Derivates. Beijing: Standards Press of China, 2018. (in Chinese)[10] 农业农村部公告第323号-4-2020. 转基因植物及其产品成分检测耐除草剂棉花MON88701及其衍生品种定性PCR方法. 北京: 中国标准出版社, 2020. Ministry of Agriculture and Rural Affairs Announcement No.323-4-2020. Detection of Genetically Modified Plants and Derived Products-qualitative PCR Method for Herbicide-tolerant Cotton MON88701 and Its Derivates. Beijing: Standards Press of China, 2020. (in Chinese)[11] 农业农村部公告第323号-5-2020. 转基因植物及其产品成分检测抗虫大豆MON87751及其衍生品种定性PCR方法. 北京: 中国标准出版社, 2020. Ministry of Agriculture and Rural Affairs Announcement No.323-5-2020. Detection of Genetically Modified Plants and Derived Products-qualitative PCR Method for Insect Resistant Soybean MON87751 and Its Derivates. Beijing: Standards Press of China, 2020. (in Chinese)[12] 农业部2259号公告-4-2015. 转基因植物及其产品成分检测定性PCR方法制定指南. 北京: 中国标准出版社, 2015. Department of Agriculture Notice 2259-4-2015. Detection of Genetically Modified Plants and Derived Products—Guidelines for Establishing qualitative PCR Method. Beijing: Standards Press of China, 2015. (in Chinese)[13] ENGL. Definition of Minimum Performance Requirements for Analytical Methods of GMO Testing. European Network of GMO Laboratories, 2015.[14] SN/T 4561-2016. 转基因检测非标方法确认评价指南. 北京: 中国标准出版社, 2016. SN/T 4561-2016. Guideline for Validation of Genetically-Modified Organism Test Methods. Beijing: Standards Press of China, 2016. (in Chinese)[15] 袁磊, 孙红炜, 杨崇良. 转基因玉米MON88017旁侧序列分析及定性PCR检测. 作物学报, 2010, 36: 361-364.
doi: 10.3724/SP.J.1006.2010.00361 Yuan L, Sun H W, Yang C L. Analysis of junction sequence the transgenic maize MON88017 and the methods of qualitative PCR detection. Acta Agron Sin, 2010, 36: 361-364. (in Chinese with English abstract)
doi: 10.3724/SP.J.1006.2010.00361[16] 申爱娟, 陈松, 周晓婴, 戚存扣. 转基因油菜W-4 T-DNA旁侧序列分析与事件特异性检测. 江苏农业学报, 2014, 30(1): 10-20. Shen A J, Chen S, Zhou X Y, Qi C K. Analysis of the flanking sequence and event-specific detection of transgenic line W-4 of Brassica napus. J Agric Sci, 2014, 30(1): 10-20. (in Chinese with English abstract)[17] 张艳敏, 张红梅, 相金英, 郭秀林, 刘子会, 李国良, 陈受宜. 转BADH基因苜蓿T-DNA侧翼序列分析及转化事件特异性分析. 作物学报, 2011, 37: 397-404.
doi: 10.3724/SP.J.1006.2011.00397 Zhang Y M, Zhang H M, Xiang J Y, Guo X L, Liu Z H, Li G L, Chen S Y. Analysis of T-DNA flanking sequences and event specific detection of transgenic alfalfa with gene BADH. Acta Agron Sin, 2011, 37: 397-404. (in Chinese with English abstract)[18] 冯翠莲, 万玥, 冯小艳, 王俊刚, 赵婷婷, 王文治, 沈林波, 张树珍. 转基因甘蔗BtG-2的T-DNA侧翼序列分析及其转化事件特异性检测. 热带作物学报, 2021, 42: 2468-2477. Feng C L, Wan Y, Feng X Y, Wang J G, Zhao T T, Wang W Z, Shen L B, Zhang S Z. Analysis of the T-DNA Flanking sequence and event-specific detection for insect-resistant transgenic sugarcane BtG-2. Chin J Trop Crops, 2021, 42: 2468-2477. (in Chinese with English abstract)[19] Marmiroli N, Maestri E, Gulli M. Methods for detection of GMOs in food and feed. Anal Bioanal Chem, 2008, 392: 369-384.
doi: 10.1007/s00216-008-2303-6pmid: 18726090[20] 农业部2122号公告-8-2014. 转基因植物及其产品成分检测抗虫水稻TT51-1及其衍生品种定量PCR方法. 北京: 中国标准出版社, 2014. Department of Agriculture Notice 2122-8-2014. Detection of Genetically Modified Plants and Derived Products-quantitative PCR Method for Insect Resistant Rice TT51-1 and Its Derivates. Beijing: Standards Press of China, 2014. (in Chinese)[21] 农业农村部公告第323号-10-2020. 转基因植物及其产品成分检测耐除草剂大豆GTS40-3-2及其衍生品种定量PCR方法. 北京: 中国标准出版社, 2020. Ministry of Agriculture and Rural Affairs Announcement No.323-5-2020. Detection of Genetically Modified Plants and Derived Products-quantitative PCR Method for Herbicide-tolerant Soybean GTS40-3-2 and Its Derivates. Beijing: Standards Press of China, 2020. (in Chinese)[22] GB/T 38132-2019. 转基因植物品系定量检测数字PCR法. 北京: 中国标准出版社, 2019. GB/T 38132-2019. Quantitative Determination of Genetically Modified Plants by Digital PCR Method. Beijing: Standards Press of China, 2019. (in Chinese)[23] SN/T 5334.2-2020. 转基因植物产品的数字PCR检测方法第二部分:转基因大豆. 北京: 中国标准出版社, 2020. SN/T 5334.2-2020. Protocol of Digital PCR for Quantitatively Detecting Genetically Modified Plants and Their Derived Products-Part 2: Genetically Modified Soybean. Beijing: Standards Press of China, 2020. (in Chinese)
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