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低温诱导拟南芥幼苗花青素积累的分子机制

来源：花匠小妙招时间：2025-12-01 18:36

CBF (C-repeat binding factor)是低温信号的核心转录因子, 在植物适应低温过程中发挥着非常重要的作用。为了探究CBF调控花青素积累的分子机制, 以拟南芥(Arabidopsis thaliana)为研究对象, 通过分子生物学和遗传学方法展开研究。表型和定量检测结果表明, 随着温度的降低, 野生型幼苗中花青素的积累量明显升高, 一些花青素合成相关基因的表达水平也显著增加。低温条件下, 与野生型相比, CBF双突变体和三突变体幼苗中花青素的积累量与部分花青素合成相关基因的表达水平显著降低。相反, CBF基因过表达后, 花青素的积累量和部分花青素合成相关基因的表达水平显著提高。蛋白质相互作用结果显示, CBF1、CBF2、CBF3均能与MYB75、MYB90、MYB113、TT8等花青素合成调控蛋白形成复合物。遗传学分析显示, 低温条件下, CBF促进花青素的积累依赖于MYB和TT8。转录激活实验分析显示, CBF能够增强MYB75和TT8的转录功能, 进而调控下游DFR (dihydroflavonol 4-reductase)和LDOX (leucoanthocyanidin dioxygenase)的表达。综上所述, 低温促进拟南芥幼苗花青素积累可能是通过CBF与 MYB、TT8的相互作用来实现的。该研究有助于进一步解析CBF蛋白的生物学功能, 以及深入理解CBF在植物低温环境适应性中的调控机制。

Abstract

C-repeat binding factors (CBFs) are the core transcription factors of low-temperature signal, which play a very important role in the process of plant adaptation to low temperature. In this study, the molecular mechanism of CBFs regulating anthocyanin accumulation in Arabidopsis thaliana was explored through the methods of molecular biology and genetics. The phenotype and quantitative test results showed that, with the decrease of temperature, the accumulation of anthocyanin in wild type seedlings increased significantly, and the expression of some genes related to anthocyanin synthesis also increased significantly. Anthocyanin ac-cumulation and the expression of some anthocyanin synthesis-related genes in CBF double mutant and triple mutant seedlings were significantly decreased at low temperature. On the contrary, after overexpression of the CBF genes, the accumulation of anthocyanin and the expression of some anthocyanin synthesis-related genes were significantly increased. The results of protein interaction showed that CBF1, CBF2 and CBF3 could form complexes with anthocyanin regulatory proteins such as MYB75, MYB90, MYB113 and TT8. Genetic analysis showed that, at low temperature, CBFs were dependent on MYB and TT8 to promote anthocyanin accumulation. Transcriptional activation assay showed that CBFs could enhance the transcriptional function of MYB75 and TT8, regulating downstream dihydroflavonol-4-reductase (DFR) and leucoanthocyanidin dioxyge-nase (LDOX) expression. In conclusion, low temperature may promote anthocyanin accumulation in Arabidop-sis seedlings through the interaction of CBFs with MYB and TT8. This study provides a further understanding of the biological function of CBF proteins and their roles in regulation of plant adaptability to low tempera-ture environment.

关键词

拟南芥 /低温信号 /CBF转录因子 /花青素 /MBW复合体{{custom_keyword}} /

Key words

Arabidopsis thaliana /low-temperature signal /CBF transcription factor /anthocyanin /MBW com-plex{{custom_keyword}} /

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黄兴连, 叶静文, 胡彦如.低温诱导拟南芥幼苗花青素积累的分子机制[J]. 生命科学研究, 2024, 28(6): 537-551 https://doi.org/10.16605/j.cnki.1007-7847.2024.11.0197

HUANG Xinglian, YE Jingwen, HU Yanru.Molecular Mechanism of Anthocyanin Accumulation Induced by Low Temperature in Arabidopsis Seedlings[J]. Life Science Research, 2024, 28(6): 537-551 https://doi.org/10.16605/j.cnki.1007-7847.2024.11.0197

中图分类号：Q945

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