首页分享揭示啶虫脒对蜜蜂神经氧化还原平衡的急性亚致死效应

揭示啶虫脒对蜜蜂神经氧化还原平衡的急性亚致死效应

来源：花匠小妙招时间：2025-11-10 18:42

AbstractUnderstanding the off-target effects of neonicotinoid insecticides, including acetamiprid, which is the most commonly applied agricultural chemical, is crucial as it may be an important factor of negative impact on pollinator insects causing a number of problems such as colony collapse disorder (CCD) of honey bees.

摘要了解新烟碱类杀虫剂（包括啶虫脒）的脱靶效应是至关重要的，啶虫脒是最常用的农用化学品，因为它可能是对传粉昆虫产生负面影响的重要因素，导致许多问题，如蜂群衰竭障碍（CCD）蜜蜂。

While CCD is known as a multifactorial disease, the role of pesticides in this context is not negligible. Therefore, it is essential to gain a deeper comprehension of the mechanisms through which they function. The aim of this research was to study the effects of sublethal acetamiprid doses on honey bees, specifically focusing on the redox homeostasis of the brain.

虽然CCD被称为多因素疾病，但农药在这方面的作用不容忽视。因此，必须更深入地了解它们发挥作用的机制。这项研究的目的是研究亚致死啶虫脒剂量对蜜蜂的影响，特别关注大脑的氧化还原稳态。

According to our findings, it can be confirmed that acetamiprid detrimentally impacts the redox balance of the brain increasing hydrogen peroxide and malondialdehyde levels, suggesting consequential lipid peroxidation and membrane damage as consequences. Moreover, acetamiprid had negative effects on the glutathione system and total antioxidant capacity, as well as key enzymes involved in the maintenance of redox homeostasis.

根据我们的研究结果，可以证实啶虫脒会对大脑的氧化还原平衡产生不利影响，从而增加过氧化氢和丙二醛的水平，从而导致脂质过氧化和膜损伤。此外，啶虫脒对谷胱甘肽系统和总抗氧化能力以及参与维持氧化还原稳态的关键酶具有负面影响。

In summary, it can be concluded that acetamiprid adversely affected the redox balance of the central nervous system of honey bees in our study. Our findings could potentially contribute to a better understanding of pesticide-related consequences and to improvement of bee health..

总之，可以得出结论，在我们的研究中，啶虫脒对蜜蜂中枢神经系统的氧化还原平衡产生不利影响。我们的研究结果可能有助于更好地了解农药相关的后果，并改善蜜蜂的健康。。

IntroductionOne of the most significant pollinator species globally, the western honey bee (Apis mellifera) is economically important and is in charge of pollinating 87.5% of flowering plants1. A number of factors have contributed to the decline in honey bee populations and other pollinating insect species in recent decades2.

引言西方蜜蜂（Apis mellifera）是全球最重要的授粉物种之一，在经济上很重要，负责87.5%的开花植物的授粉1。近十年来，许多因素导致蜜蜂种群和其他授粉昆虫物种的减少2。

These factors include habitat degradation and shrinkage, crop monoculture, excessive pesticide use, the presence of pathogens and parasites, inadequate forage nutrient content, and the detrimental impacts of climate change3. Subsequently in the 2000s, honey bee colonies have been affected by CCD, a serious issue that was initially identified in the United States and then identified in Europe a few years later.

这些因素包括栖息地退化和萎缩，作物单一种植，农药过量使用，病原体和寄生虫的存在，饲料养分含量不足以及气候变化的不利影响3。随后在2000年代，蜜蜂群落受到CCD的影响，这是一个严重的问题，最初在美国发现，几年后在欧洲发现。

The disorder has subsequently expanded throughout the world4. Since then, a significant amount of research has been performed regarding the topic, leading to the conclusion that several factors must be taken into account rather than just one5. These include parasitic infections, pathogenic bacteria or viruses, pesticide exposure, temperature fluctuations, crowded living arrangements, a lack of pollen and nectar, modification of the sensitive bee microbiome as a result of inadequate foraging, and scarce or contaminated water sources6.

这种疾病随后在全世界范围内蔓延4。从那时起，就这个话题进行了大量的研究，得出的结论是必须考虑几个因素，而不仅仅是一个因素5。这些包括寄生虫感染，病原菌或病毒，农药暴露，温度波动，拥挤的生活安排，缺乏花粉和花蜜，由于觅食不足而改变敏感的蜜蜂微生物群，以及稀缺或受污染的水源6。

Regarding parasites, not all infestations have been reported in the impacted colonies; nonetheless, it is important to draw attention to the infection with the Varroa destructor mite and the unicellular parasite Nosema ceranae, which are suggested to be predisposing factors7.Understanding the etiology and causes of CCD requires research on the effects of certain insecticides such as neonicotinoids.

关于寄生虫，在受影响的殖民地中并没有报告所有的侵染；尽管如此，重要的是要提请注意瓦罗破坏螨和单细胞寄生虫金枪鱼微孢子虫的感染，这被认为是诱发因素7。了解CCD的病因和原因需要研究某些杀虫剂（如新烟碱类）的作用。

Pest control has become increasingly important recently as agriculture has grown quickly. Imidacloprid, the first co.

随着农业的快速发展，害虫防治变得越来越重要。吡虫啉，第一个co。

Data availability

数据可用性

All datasets are available through the following link: https://doi.org/https://doi.org/10.6084/m9.figshare.26346565.

所有数据集均可通过以下链接获得：https://doi.org/https://doi.org/10.6084/m9.figshare.26346565.

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Download referencesAcknowledgementsSpecial thanks must be given to the apiary of Ildikó Mackeiné Aux and József Mackei for providing the laboratory animals and to Szilvia Pálinkás for her support and professional help in the laboratory measurements.FundingProject no. FK146534 has been implemented with the support provided by the Ministry of Culture and Innovation of Hungary from the National Research, Development and Innovation Fund, financed under the NKFIH OTKA FK 23 funding scheme.

下载参考文献致谢特别感谢IldikóMackeinéAux和József Mackei的养蜂场提供了实验动物，并感谢Szilvia Pálinkás在实验室测量中的支持和专业帮助。资助项目FK146534由匈牙利文化和创新部提供支持，由国家研究、发展和创新基金资助，由NKFIH OTKA FK 23资助计划资助。

The study was supported by the ÚNKP-23-2-I-ÁTE-8 New National Excellence Program of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund. Project no. RRF-2.3.1-21-2022–00001 has been implemented with the support provided by the Recovery and Resilience Facility (RRF), financed under the National Recovery Fund budget estimate, RRF-2.3.1-21 funding scheme.

这项研究得到了国家研究、发展和创新基金来源的文化与创新部的ÚNKP-23-2-I-TE-8新国家卓越计划的支持。项目编号RRF-2.3.1-21-2022-00001已在恢复和复原基金（RRF）的支持下实施，该基金由国家恢复基金预算估算RRF-2.3.1-21资助计划资助。

This study was supported by the strategic research fund of the University of Veterinary Medicine Budapest (Grant No. SRF-001.).Author informationAuthors and AffiliationsDivision of Biochemistry, Department of Physiology and Biochemistry, University of Veterinary Medicine Budapest, István Street 2, Budapest, 1078, HungaryMáté Mackei, Fanni Huber, Csilla Sebők, Júlia Vöröházi, Patrik Tráj, Rege Anna Márton, Evelin Horváth, Zsuzsanna Neogrády & Gábor MátisNational Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, University of Veterinary Medicine Budapest, István Street 2, Budapest, 1078, HungaryMáté Mackei, Rege Anna Márton & Gábor MátisAuthorsMáté MackeiView author publicationsYou can also search for this author in.

这项研究得到了布达佩斯兽医大学战略研究基金的支持（批准号SRF-001）。作者信息作者和附属机构布达佩斯兽医大学生理学和生物化学系生物化学系，布达佩斯伊斯特万街2号，1078年，HungaryMátéMackei，Fanni Huber，Csilla Sebők，Júlia Vöröházi，Patrik TráJ，Rege Anna Márton，Evelin Horváth，Zsuzzanna Neogrády&Gábor Mátis传染病，抗菌素耐药性，兽医公共卫生和食品链安全国家实验室，布达佩斯兽医大学，伊斯特万街2号，布达佩斯Apest，1078，HungaryMátéMackei，Rege Anna Márton＆Gábor MátisAuthorsMátéMackeiView作者出版物您也可以在中搜索这位作者。

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PubMed Google ScholarContributionsMáté Mackei: Conceptualization, Methodology, Formal analysis, Investigation, Resources, Data curation, Project administration, Writing original draft, Writing review and editing, Software, Visualization, Supervision, Validation; Fanni Huber: Formal analysis, Investigation, Writing original draft, Software, Visualization; Csilla Sebők: Methodology, Supervision; Júlia Vörösházi: Investigation, Validation; Patrik Tráj: Investigation, Validation, Methodology; Rege Márton: Investigation, Formal analysis; Evelin Horváth: Investigation, Formal analysis; Zsuzsanna Neogrády: Conceptualization, Methodology, Resources, Writing review and editing, Supervision; Gábor Mátis: Conceptualization, Methodology, Investigation, Funding acquisition, Resources, Writing—Original Draft, Software, Visualization, Supervision.Corresponding authorCorrespondence to.

PubMed Google ScholarContributionsMátéMackei：概念化，方法论，形式分析，调查，资源，数据管理，项目管理，撰写原稿，撰写评论和编辑，软件，可视化，监督，验证；；Csilla Sebők：方法论，监督；Júlia Vörösházi：调查，验证；帕特里克·特拉杰：调查，验证，方法论；雷格·马尔顿：调查，形式分析；Evelin Horváth：调查，形式分析；Zsuzzanna Neogrady：概念化，方法论，资源，写作评论和编辑，监督；Gábor Mátis：概念化，方法论，调查，资金获取，资源，撰写原始草案，软件，可视化，监督。对应作者对应。

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Reprints and permissionsAbout this articleCite this articleMackei, M., Huber, F., Sebők, C. et al. Unraveling the acute sublethal effects of acetamiprid on honey bee neurological redox equilibrium.

转载和许可本文引用本文Mackei，M.，Huber，F.，Sebők，C。等人揭示了啶虫脒对蜜蜂神经氧化还原平衡的急性亚致死作用。

Sci Rep 14, 27514 (2024). https://doi.org/10.1038/s41598-024-79274-6Download citationReceived: 22 July 2024Accepted: 07 November 2024Published: 11 November 2024DOI: https://doi.org/10.1038/s41598-024-79274-6Share this articleAnyone you share the following link with will be able to read this content:Get shareable linkSorry, a shareable link is not currently available for this article.Copy to clipboard.

Sci Rep 1427514（2024）。https://doi.org/10.1038/s41598-024-79274-6Download引文接收日期：2024年7月22日接受日期：2024年11月7日发布日期：2024年11月11日OI：https://doi.org/10.1038/s41598-024-79274-6Share本文与您共享以下链接的任何人都可以阅读此内容：获取可共享链接对不起，本文目前没有可共享的链接。复制到剪贴板。

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KeywordsApis melliferaNeonicotinoidsOxidative stressGlutathioneMalondialdehydeColony collapse disorder

关键词SAPIS melliferaNeonicotinoidsOxidative stress谷胱甘肽二醛克隆衰竭障碍