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碳饥饿花药介导的光周期依赖型水稻雄性育性的调控作用

来源：花匠小妙招时间：2025-11-10 01:05

碳饥饿花药介导的光周期依赖型水稻雄性育性的调控作用

The regulatory role of CARBON STARVED ANTHER-mediated photoperiod-dependent male fertility in rice.

作者信息

Li Jingbin, Wang Duoxiang, Sun Shiyu, Sun Linlin, Zong Jie, Lei Yaqi, Yu Jing, Liang Wanqi, Zhang Dabing

机构信息

Joint International Research Laboratory of Metabolic and Developmental Sciences, State Key Laboratory of Hybrid Rice, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, Australia.

出版信息

Plant Physiol. 2022 Jun 1;189(2):955-971. doi: 10.1093/plphys/kiac076.

Abstract

Environmental signals, especially daylength, play important roles in determining fertility in photoperiod-sensitive genic male sterile (PGMS) lines that are critical to sustain production of high-yielding hybrid rice (Oryza sativa) varieties. However, the mechanisms by which PGMS lines perceive changes in photoperiod and transmit those signals to elicit downstream effects are not well understood. In this study, we compared the transcriptomes from the leaves and anthers of carbon starved anther (csa), a PGMS line, to wild-type (WT) tissues under different photoperiods. Components of circadian clock in the leaves, including Circadian Clock-Associated 1 and Pseudo-Response Regulator (PRR95), played vital roles in sensing the photoperiod signals. Photoperiod signals were weakly transduced to anthers, where gene expression was mainly controlled by the CSA allele. CSA played a critical role in regulating sugar metabolism and cell wall synthesis in anthers under short-day conditions, and transcription of key genes inducing csa-directed sterility was upregulated under long-day (LD) conditions though not to WT levels, revealing a mechanism to explain the partial restoration of fertility in rice under LD conditions. Eight direct targets of CSA regulation were identified, all of which were genes involved in sugar metabolism and transport (cell wall invertases, SWEETs, and monosaccharide transporters) expressed only in reproductive tissues. Several hub genes coordinating the effects of CSA regulation were identified as critical elements determining WT male fertility and further analysis of these and related genes will reveal insights into how CSA coordinates sugar metabolism, cell wall biosynthesis, and photoperiod sensing in rice anther development.

摘要

环境信号，尤其是日照长度，在决定光周期敏感型雄性不育（PGMS）系的育性方面发挥着重要作用，而PGMS系对于维持高产杂交水稻（Oryza sativa）品种的生产至关重要。然而，PGMS系感知光周期变化并传递这些信号以引发下游效应的机制尚未得到充分了解。在本研究中，我们比较了PGMS系碳饥饿花药（csa）的叶片和花药与野生型（WT）组织在不同光周期下的转录组。叶片中的生物钟组件，包括生物钟关联蛋白1和伪响应调节因子（PRR95），在感知光周期信号中发挥着至关重要的作用。光周期信号微弱地传递到花药，花药中的基因表达主要受CSA等位基因控制。CSA在短日条件下调节花药中的糖代谢和细胞壁合成中起关键作用，在长日（LD）条件下，诱导csa定向不育的关键基因转录上调，尽管未达到野生型水平，揭示了一种机制来解释水稻在长日条件下育性的部分恢复。确定了CSA调控的八个直接靶点，所有这些都是仅在生殖组织中表达的参与糖代谢和转运的基因（细胞壁转化酶、SWEETs和单糖转运蛋白）。确定了几个协调CSA调控效应的枢纽基因，作为决定野生型雄性育性的关键因素，对这些基因及相关基因的进一步分析将揭示CSA如何在水稻花药发育中协调糖代谢、细胞壁生物合成和光周期感知的见解。